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Cytochrome P450 CYP4DE1 and CYP6CW3v2 contribute to ethiprole resistance in Laodelphax striatellus (Fallén).
Insect Mol Biol. 2015 Jun; 24(3):368-76.IM

Abstract

Laodelphax striatellus Fallén (Hemiptera: Delphacidae), a destructive pest of rice, has developed high resistance to multiple insecticides, threatening the success of pest management programmes. The present study investigated ethiprole resistance mechanisms in a field population that is highly resistant to ethiprole. That population was used to establish a laboratory population that was subjected to further selection to produce a resistant strain. Target genes were cloned and compared between the resistant and the susceptible strains, the role of detoxification enzymes was examined, and the relative expression levels of 71 detoxification enzyme genes were tested using quantitative real time (RT)-PCR. The laboratory selection enhanced the resistance from 107-fold to 180-fold. The Rdl-type target site mutation seldom occurred in the resistant strain and is unlikely to represent the major mechanism underlying the observed resistance. Of the three important detoxification enzymes, only P450 monooxygenase was found to be associated with ethiprole resistance. Moreover, two genes, CYP4DE1 and CYP6CW3v2, were found to be overexpressed in the resistant strain. Furthermore, gene-silencing via a double-stranded RNA feeding test was carried out, and the results showed that the mRNA levels of CYP4DE1 and CYP6CW3v2 were reduced in the resistant strain, whereas ethiprole susceptibility was increased. These results suggest that CYP4DE1 and CYP6CW3v2 play an important role in ethiprole resistance in L. striatellus.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Elzaki ME
Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Jiangsu/Key Laboratory of Monitoring and Management of Plant Diseases and Insects, Ministry of Agriculture, Nanjing, Jiangsu, 210095, China.
Zhang W
No affiliation info available
Han Z
No affiliation info available

MeSH

Amino Acid SequenceAnimalsCytochrome P-450 Enzyme SystemHemipteraInsect ProteinsInsecticide ResistanceInsecticidesMolecular Sequence DataPyrazolesRNA InterferenceRNA, Double-Stranded

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25693611

Citation

Elzaki, M E A., et al. "Cytochrome P450 CYP4DE1 and CYP6CW3v2 Contribute to Ethiprole Resistance in Laodelphax Striatellus (Fallén)." Insect Molecular Biology, vol. 24, no. 3, 2015, pp. 368-76.
Elzaki ME, Zhang W, Han Z. Cytochrome P450 CYP4DE1 and CYP6CW3v2 contribute to ethiprole resistance in Laodelphax striatellus (Fallén). Insect Mol Biol. 2015;24(3):368-76.
Elzaki, M. E., Zhang, W., & Han, Z. (2015). Cytochrome P450 CYP4DE1 and CYP6CW3v2 contribute to ethiprole resistance in Laodelphax striatellus (Fallén). Insect Molecular Biology, 24(3), 368-76. https://doi.org/10.1111/imb.12164
Elzaki ME, Zhang W, Han Z. Cytochrome P450 CYP4DE1 and CYP6CW3v2 Contribute to Ethiprole Resistance in Laodelphax Striatellus (Fallén). Insect Mol Biol. 2015;24(3):368-76. PubMed PMID: 25693611.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cytochrome P450 CYP4DE1 and CYP6CW3v2 contribute to ethiprole resistance in Laodelphax striatellus (Fallén). AU - Elzaki,M E A, AU - Zhang,W, AU - Han,Z, Y1 - 2015/02/18/ PY - 2015/2/20/entrez PY - 2015/2/20/pubmed PY - 2016/3/19/medline KW - Laodelphax Striatellus KW - RNA interference KW - cytochrome P450 KW - ethiprole KW - resistance SP - 368 EP - 76 JF - Insect molecular biology JO - Insect Mol Biol VL - 24 IS - 3 N2 - Laodelphax striatellus Fallén (Hemiptera: Delphacidae), a destructive pest of rice, has developed high resistance to multiple insecticides, threatening the success of pest management programmes. The present study investigated ethiprole resistance mechanisms in a field population that is highly resistant to ethiprole. That population was used to establish a laboratory population that was subjected to further selection to produce a resistant strain. Target genes were cloned and compared between the resistant and the susceptible strains, the role of detoxification enzymes was examined, and the relative expression levels of 71 detoxification enzyme genes were tested using quantitative real time (RT)-PCR. The laboratory selection enhanced the resistance from 107-fold to 180-fold. The Rdl-type target site mutation seldom occurred in the resistant strain and is unlikely to represent the major mechanism underlying the observed resistance. Of the three important detoxification enzymes, only P450 monooxygenase was found to be associated with ethiprole resistance. Moreover, two genes, CYP4DE1 and CYP6CW3v2, were found to be overexpressed in the resistant strain. Furthermore, gene-silencing via a double-stranded RNA feeding test was carried out, and the results showed that the mRNA levels of CYP4DE1 and CYP6CW3v2 were reduced in the resistant strain, whereas ethiprole susceptibility was increased. These results suggest that CYP4DE1 and CYP6CW3v2 play an important role in ethiprole resistance in L. striatellus. SN - 1365-2583 UR - https://www.unboundmedicine.com/medline/citation/25693611/Cytochrome_P450_CYP4DE1_and_CYP6CW3v2_contribute_to_ethiprole_resistance_in_Laodelphax_striatellus__Fallén__ DB - PRIME DP - Unbound Medicine ER -