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Enhanced antiproliferative and apoptosis effect of paclitaxel-loaded polymeric micelles against non-small cell lung cancers.
Tumour Biol 2015; 36(7):4949-59TB

Abstract

Amphiphilic copolymer monomethoxy poly(ethylene glycol)-poly(caprolactone)-D-α-tocopheryl polyethylene glycol 1000 succinate (MPEG-PCL-TPGS) was prepared. In the present study, MPEG-PCL-TPGS was used as a novel nanovehicle for the delivery of paclitaxel (PTX) in the treatment of resistant lung cancers. The PTX-loaded MPEG-PCL-TPGS (PTX/MPT) micelles exhibited sustained release profile (168 h) with accelerated drug release at acidic pH conditions. The blank polymeric micelles showed excellent biocompatibility with cell viability of >85 %, making it suitable for all in vivo applications. PTX/MPT micelles displayed superior cytotoxicity in A-549 lung cancer cells than that of free PTX. The selective delivery of PTX to cancer cells resulted in enhanced cancer cell death. The PTX/MPT micelles showed higher cellular uptake via endocytosis pathways. The PTX-bound micelles preferentially arrested the cells at G2/M phase and showed a marked increase in sub G1 cell population (∼ 20 %). The pharmacokinetic study revealed a long blood circulation for PTX/MPT micelles. Finally, micellar formulation showed a remarkable tumor suppression effect in resistant A549/Taxol cells bearing xenograft nude mice along with no toxicity profile. The results indicate that the PTX-loaded biocompatible polymeric nanosystem could act as a potential delivery system for the treatment of lung carcinomas.

Authors+Show Affiliations

National Hepatobiliary and Enteric Surgery Research Center, Ministry of Health, Xiangya Hospital, Central South University, No. 87 Xiangya Road, Changsha, Hunan, 410008, China.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25702089

Citation

Zhang, Xiao-Ying, and Yang-De Zhang. "Enhanced Antiproliferative and Apoptosis Effect of Paclitaxel-loaded Polymeric Micelles Against Non-small Cell Lung Cancers." Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine, vol. 36, no. 7, 2015, pp. 4949-59.
Zhang XY, Zhang YD. Enhanced antiproliferative and apoptosis effect of paclitaxel-loaded polymeric micelles against non-small cell lung cancers. Tumour Biol. 2015;36(7):4949-59.
Zhang, X. Y., & Zhang, Y. D. (2015). Enhanced antiproliferative and apoptosis effect of paclitaxel-loaded polymeric micelles against non-small cell lung cancers. Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine, 36(7), pp. 4949-59. doi:10.1007/s13277-015-3142-7.
Zhang XY, Zhang YD. Enhanced Antiproliferative and Apoptosis Effect of Paclitaxel-loaded Polymeric Micelles Against Non-small Cell Lung Cancers. Tumour Biol. 2015;36(7):4949-59. PubMed PMID: 25702089.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enhanced antiproliferative and apoptosis effect of paclitaxel-loaded polymeric micelles against non-small cell lung cancers. AU - Zhang,Xiao-Ying, AU - Zhang,Yang-De, Y1 - 2015/02/22/ PY - 2014/12/07/received PY - 2015/01/19/accepted PY - 2015/2/23/entrez PY - 2015/2/24/pubmed PY - 2015/10/27/medline SP - 4949 EP - 59 JF - Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine JO - Tumour Biol. VL - 36 IS - 7 N2 - Amphiphilic copolymer monomethoxy poly(ethylene glycol)-poly(caprolactone)-D-α-tocopheryl polyethylene glycol 1000 succinate (MPEG-PCL-TPGS) was prepared. In the present study, MPEG-PCL-TPGS was used as a novel nanovehicle for the delivery of paclitaxel (PTX) in the treatment of resistant lung cancers. The PTX-loaded MPEG-PCL-TPGS (PTX/MPT) micelles exhibited sustained release profile (168 h) with accelerated drug release at acidic pH conditions. The blank polymeric micelles showed excellent biocompatibility with cell viability of >85 %, making it suitable for all in vivo applications. PTX/MPT micelles displayed superior cytotoxicity in A-549 lung cancer cells than that of free PTX. The selective delivery of PTX to cancer cells resulted in enhanced cancer cell death. The PTX/MPT micelles showed higher cellular uptake via endocytosis pathways. The PTX-bound micelles preferentially arrested the cells at G2/M phase and showed a marked increase in sub G1 cell population (∼ 20 %). The pharmacokinetic study revealed a long blood circulation for PTX/MPT micelles. Finally, micellar formulation showed a remarkable tumor suppression effect in resistant A549/Taxol cells bearing xenograft nude mice along with no toxicity profile. The results indicate that the PTX-loaded biocompatible polymeric nanosystem could act as a potential delivery system for the treatment of lung carcinomas. SN - 1423-0380 UR - https://www.unboundmedicine.com/medline/citation/25702089/Enhanced_antiproliferative_and_apoptosis_effect_of_paclitaxel_loaded_polymeric_micelles_against_non_small_cell_lung_cancers_ L2 - https://link.springer.com/article/10.1007/s13277-015-3142-7 DB - PRIME DP - Unbound Medicine ER -