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Ameliorative effect of methanol extract of Rumex vesicarius on CCl4-induced liver damage in Wistar albino rats.
Pharm Biol. 2015 Aug; 53(8):1163-7.PB

Abstract

CONTEXT

Rumex vesicarius L. (Polygonaceae), an edible plant, is reported to have many bioactive phytochemicals, especially flavonoids and anthraquinones with antioxidant and detoxifying properties.

OBJECTIVE

This study evaluated the methanolic extract of R. vasicarius (MERV) for hepatoprotective activity in rats against CCl4-induced liver damage.

MATERIALS AND METHODS

The whole plant extract was prepared and investigated for its hepatoprotective activity. Rats were pretreated with MERV (100 and 200 mg/kg, p.o.) for 7 d prior to the induction of liver damage by CCl4. Animals were then sacrificed 24 h after CCl4 administration for the biochemical (AST, ALT, and ALP activity in serum; lipid peroxidation (LPO) and glutathione (GSH) levels in liver tissue) and histological analyses.

RESULTS

CCl4-induced hepatotoxicity was confirmed by an increase (p < 0.05) in serum AST (4.55-fold), ALT (3.51-fold), and ALP (1.82-fold) activities. CCl4-induced hepatotoxicity was also manifested by an increase (p < 0.05) in LPO (3.88-fold) and depletion of reduced glutathione (3.14-fold) activity in liver tissue. The multiple dose MERV administration at 200 mg/kg showed promising hepatoprotective activity as evident from significant decrease levels of serum AST (230.01 ± 13.21), serum ALT (82.15 ± 5.01), serum ALP (504.75 ± 19.72), hepatic LPO (3.38 ± 0.33), and increased levels of hepatic glutathione (0.34 ± 0.04) towards near normal. Further, biochemical results were confirmed by histopathological changes as compared with CCl4-intoxicated rats.

DISCUSSION AND CONCLUSION

The results obtained from this study indicate hepatoprotective activity of Rumex plant against CCl4-induced liver toxicity; hence, it can be used as a hepatoprotective agent.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Ganaie MA
Department of Pharmacology, College of Pharmacy, Salman Bin Abdulaziz University , Al-Kharj , Kingdom of Saudi Arabia and.
Khan TH
No affiliation info available
Siddiqui NA
No affiliation info available
Ansari MN
No affiliation info available

MeSH

AnimalsCarbon TetrachlorideChemical and Drug Induced Liver InjuryMaleMethanolPlant ExtractsRatsRats, WistarRumexTreatment Outcome

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25702903

Citation

Ganaie, Majid Ahmad, et al. "Ameliorative Effect of Methanol Extract of Rumex Vesicarius On CCl4-induced Liver Damage in Wistar Albino Rats." Pharmaceutical Biology, vol. 53, no. 8, 2015, pp. 1163-7.
Ganaie MA, Khan TH, Siddiqui NA, et al. Ameliorative effect of methanol extract of Rumex vesicarius on CCl4-induced liver damage in Wistar albino rats. Pharm Biol. 2015;53(8):1163-7.
Ganaie, M. A., Khan, T. H., Siddiqui, N. A., & Ansari, M. N. (2015). Ameliorative effect of methanol extract of Rumex vesicarius on CCl4-induced liver damage in Wistar albino rats. Pharmaceutical Biology, 53(8), 1163-7. https://doi.org/10.3109/13880209.2014.967782
Ganaie MA, et al. Ameliorative Effect of Methanol Extract of Rumex Vesicarius On CCl4-induced Liver Damage in Wistar Albino Rats. Pharm Biol. 2015;53(8):1163-7. PubMed PMID: 25702903.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ameliorative effect of methanol extract of Rumex vesicarius on CCl4-induced liver damage in Wistar albino rats. AU - Ganaie,Majid Ahmad, AU - Khan,Tajdar Husain, AU - Siddiqui,Nasir Ali, AU - Ansari,Mohd Nazam, Y1 - 2015/02/23/ PY - 2015/2/24/entrez PY - 2015/2/24/pubmed PY - 2016/3/2/medline KW - Antioxidant KW - aspartate transaminase KW - hepatotoxicity KW - liver damage SP - 1163 EP - 7 JF - Pharmaceutical biology JO - Pharm Biol VL - 53 IS - 8 N2 - CONTEXT: Rumex vesicarius L. (Polygonaceae), an edible plant, is reported to have many bioactive phytochemicals, especially flavonoids and anthraquinones with antioxidant and detoxifying properties. OBJECTIVE: This study evaluated the methanolic extract of R. vasicarius (MERV) for hepatoprotective activity in rats against CCl4-induced liver damage. MATERIALS AND METHODS: The whole plant extract was prepared and investigated for its hepatoprotective activity. Rats were pretreated with MERV (100 and 200 mg/kg, p.o.) for 7 d prior to the induction of liver damage by CCl4. Animals were then sacrificed 24 h after CCl4 administration for the biochemical (AST, ALT, and ALP activity in serum; lipid peroxidation (LPO) and glutathione (GSH) levels in liver tissue) and histological analyses. RESULTS: CCl4-induced hepatotoxicity was confirmed by an increase (p < 0.05) in serum AST (4.55-fold), ALT (3.51-fold), and ALP (1.82-fold) activities. CCl4-induced hepatotoxicity was also manifested by an increase (p < 0.05) in LPO (3.88-fold) and depletion of reduced glutathione (3.14-fold) activity in liver tissue. The multiple dose MERV administration at 200 mg/kg showed promising hepatoprotective activity as evident from significant decrease levels of serum AST (230.01 ± 13.21), serum ALT (82.15 ± 5.01), serum ALP (504.75 ± 19.72), hepatic LPO (3.38 ± 0.33), and increased levels of hepatic glutathione (0.34 ± 0.04) towards near normal. Further, biochemical results were confirmed by histopathological changes as compared with CCl4-intoxicated rats. DISCUSSION AND CONCLUSION: The results obtained from this study indicate hepatoprotective activity of Rumex plant against CCl4-induced liver toxicity; hence, it can be used as a hepatoprotective agent. SN - 1744-5116 UR - https://www.unboundmedicine.com/medline/citation/25702903/Ameliorative_effect_of_methanol_extract_of_Rumex_vesicarius_on_CCl4_induced_liver_damage_in_Wistar_albino_rats_ DB - PRIME DP - Unbound Medicine ER -