Tags

Type your tag names separated by a space and hit enter

Photothermal therapeutic response of cancer cells to aptamer-gold nanoparticle-hybridized graphene oxide under NIR illumination.
ACS Appl Mater Interfaces. 2015 Mar 11; 7(9):5097-106.AA

Abstract

The objective of this study was to synthesize a nanocomposite, aptamer-gold nanoparticle-hybridized graphene oxide (Apt-AuNP-GO), to facilitate targeted treatment of tumor cells by near-infrared (NIR) light-activatable photothermal therapy. We also investigated whether Apt-AuNP-GO with NIR illumination modulates heat shock proteins (HSPs) expression leading to therapeutic response in human breast cancer cells. These findings can provide strategies for improving the photothermal therapy efficacy of cancer. The self-assembled Apt-AuNP-GO nanocomposite could selectively target MUC1-positive human breast cancer cells (MCF-7) due to the specific interaction between the MUC1-binding-aptamer and the MUC1 (type I transmembrane mucin glycoprotein) on cell membrane. In addition, Apt-AuNP-GO has a high light-to-heat conversion capability for photoabsorption of NIR light, and it is able to exert therapeutic effects on MCF-7 cells at an ultralow concentration without inducing adverse effects in healthy cells. The Apt-AuNP-GO nanocomposites combine the advantages of GOs, AuNPs, and Apts, possess specific targeting capability, excellent biocompatibility, and tumor cell destruction ability, suggesting great potential for application in the photothermal therapy of breast cancer. Under NIR illumination, Apt-AuNP-GO induced transient increase in HSP70 expression, which decreased thereafter. This phenomenon may cause irreversible damage to Apt-AuNP-GO-treated MCF-7 cell under NIR illumination. We also demonstrated that the combination therapy of heat and HSP70 inhibitor could synergistically generate marked tumoricidal effects against breast cancer. These results suggest that the degree and duration of HSP70 protein expression are correlated with therapeutic effects against breast cancer for Apt-AuNP-GO-assisted photothermal therapy. We believe that such a nanocomposite can be readily extended to the construction of HSP70 inhibitors-loaded Apt-AuNP-GO, which could deliver both heat and HSP70 inhibitors to tumorigenic regions for the chemo-photothermal therapy.

Authors+Show Affiliations

Key Laboratory of Nano-Bio Interface, Suzhou Key Laboratory for Nanotheranostics, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences , Suzhou 215123, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25705789

Citation

Yang, Lingyan, et al. "Photothermal Therapeutic Response of Cancer Cells to Aptamer-gold Nanoparticle-hybridized Graphene Oxide Under NIR Illumination." ACS Applied Materials & Interfaces, vol. 7, no. 9, 2015, pp. 5097-106.
Yang L, Tseng YT, Suo G, et al. Photothermal therapeutic response of cancer cells to aptamer-gold nanoparticle-hybridized graphene oxide under NIR illumination. ACS Appl Mater Interfaces. 2015;7(9):5097-106.
Yang, L., Tseng, Y. T., Suo, G., Chen, L., Yu, J., Chiu, W. J., Huang, C. C., & Lin, C. H. (2015). Photothermal therapeutic response of cancer cells to aptamer-gold nanoparticle-hybridized graphene oxide under NIR illumination. ACS Applied Materials & Interfaces, 7(9), 5097-106. https://doi.org/10.1021/am508117e
Yang L, et al. Photothermal Therapeutic Response of Cancer Cells to Aptamer-gold Nanoparticle-hybridized Graphene Oxide Under NIR Illumination. ACS Appl Mater Interfaces. 2015 Mar 11;7(9):5097-106. PubMed PMID: 25705789.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Photothermal therapeutic response of cancer cells to aptamer-gold nanoparticle-hybridized graphene oxide under NIR illumination. AU - Yang,Lingyan, AU - Tseng,Yu-Ting, AU - Suo,Guangli, AU - Chen,Liliang, AU - Yu,Jiantao, AU - Chiu,Wei-Jane, AU - Huang,Chih-Ching, AU - Lin,Chia-Hua, Y1 - 2015/02/27/ PY - 2015/2/24/entrez PY - 2015/2/24/pubmed PY - 2015/12/15/medline KW - HSP70 KW - aptamers KW - cancer cells KW - gold nanoparticles KW - graphene oxides KW - phototherapy SP - 5097 EP - 106 JF - ACS applied materials & interfaces JO - ACS Appl Mater Interfaces VL - 7 IS - 9 N2 - The objective of this study was to synthesize a nanocomposite, aptamer-gold nanoparticle-hybridized graphene oxide (Apt-AuNP-GO), to facilitate targeted treatment of tumor cells by near-infrared (NIR) light-activatable photothermal therapy. We also investigated whether Apt-AuNP-GO with NIR illumination modulates heat shock proteins (HSPs) expression leading to therapeutic response in human breast cancer cells. These findings can provide strategies for improving the photothermal therapy efficacy of cancer. The self-assembled Apt-AuNP-GO nanocomposite could selectively target MUC1-positive human breast cancer cells (MCF-7) due to the specific interaction between the MUC1-binding-aptamer and the MUC1 (type I transmembrane mucin glycoprotein) on cell membrane. In addition, Apt-AuNP-GO has a high light-to-heat conversion capability for photoabsorption of NIR light, and it is able to exert therapeutic effects on MCF-7 cells at an ultralow concentration without inducing adverse effects in healthy cells. The Apt-AuNP-GO nanocomposites combine the advantages of GOs, AuNPs, and Apts, possess specific targeting capability, excellent biocompatibility, and tumor cell destruction ability, suggesting great potential for application in the photothermal therapy of breast cancer. Under NIR illumination, Apt-AuNP-GO induced transient increase in HSP70 expression, which decreased thereafter. This phenomenon may cause irreversible damage to Apt-AuNP-GO-treated MCF-7 cell under NIR illumination. We also demonstrated that the combination therapy of heat and HSP70 inhibitor could synergistically generate marked tumoricidal effects against breast cancer. These results suggest that the degree and duration of HSP70 protein expression are correlated with therapeutic effects against breast cancer for Apt-AuNP-GO-assisted photothermal therapy. We believe that such a nanocomposite can be readily extended to the construction of HSP70 inhibitors-loaded Apt-AuNP-GO, which could deliver both heat and HSP70 inhibitors to tumorigenic regions for the chemo-photothermal therapy. SN - 1944-8252 UR - https://www.unboundmedicine.com/medline/citation/25705789/Photothermal_therapeutic_response_of_cancer_cells_to_aptamer_gold_nanoparticle_hybridized_graphene_oxide_under_NIR_illumination_ DB - PRIME DP - Unbound Medicine ER -