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Effects of antiviral medications on herpetic epithelial keratitis in mice.
Jpn J Ophthalmol. 2015 May; 59(3):194-200.JJ

Abstract

PURPOSE

Aciclovir (ACV), valaciclovir (VACV) and famciclovir (FCV) are used for systemic infections caused by herpes virus. In Japan, only topical ACV is permitted for use against herpetic keratitis. We investigated the effectiveness of topical ACV, oral VACV and oral FCV on mouse epithelial herpetic keratitis.

METHODS

C57/BL76 mice were inoculated with HSV-1 McKrae strain in the cornea. Once infection was confirmed 4 days after inoculation, topical ACV, oral VACV and FCV were started and administered for 5 days. Control groups were given either topical or oral saline. On days 2, 4, 6 and 10 after medication started, tears, eyeballs, and trigeminal ganglia were examined using viral culture and real-time PCR.

RESULTS

Viral culture of tears detected no HSV in the topical ACV group on day 4 after administration start; with similar results for the oral VACV group on day 4; and the oral FCV group on day 6. Real-time PCR of the eyeballs showed significant decrease of HSV DNA copy number in the topical ACV group on days 4 and 6 compared to the topical saline group. Real-time PCR of the trigeminal ganglia showed significant decrease of HSV DNA copy number in the oral VACV group on days 4 and 6, and in the oral FCV group on day 6 compared to the oral saline group.

CONCLUSION

We suggest that 5-day administration of topical ACV, oral VACV and oral FCV are effective for mouse epithelial herpetic keratitis and sufficiently decrease HSV amounts in the ocular surface and eyeballs.

Authors+Show Affiliations

Department of Ophthalmology, Kinki University Faculty of Medicine, 377-2 Ono-Higashi, Osaka-Sayama, Osaka, 589-8511, Japan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25707352

Citation

Komoto, Shohei, et al. "Effects of Antiviral Medications On Herpetic Epithelial Keratitis in Mice." Japanese Journal of Ophthalmology, vol. 59, no. 3, 2015, pp. 194-200.
Komoto S, Higaki S, Fukuda M, et al. Effects of antiviral medications on herpetic epithelial keratitis in mice. Jpn J Ophthalmol. 2015;59(3):194-200.
Komoto, S., Higaki, S., Fukuda, M., & Shimomura, Y. (2015). Effects of antiviral medications on herpetic epithelial keratitis in mice. Japanese Journal of Ophthalmology, 59(3), 194-200. https://doi.org/10.1007/s10384-015-0375-3
Komoto S, et al. Effects of Antiviral Medications On Herpetic Epithelial Keratitis in Mice. Jpn J Ophthalmol. 2015;59(3):194-200. PubMed PMID: 25707352.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of antiviral medications on herpetic epithelial keratitis in mice. AU - Komoto,Shohei, AU - Higaki,Shiro, AU - Fukuda,Masahiko, AU - Shimomura,Yoshikazu, Y1 - 2015/02/25/ PY - 2014/08/11/received PY - 2015/01/21/accepted PY - 2015/2/25/entrez PY - 2015/2/25/pubmed PY - 2016/2/6/medline SP - 194 EP - 200 JF - Japanese journal of ophthalmology JO - Jpn. J. Ophthalmol. VL - 59 IS - 3 N2 - PURPOSE: Aciclovir (ACV), valaciclovir (VACV) and famciclovir (FCV) are used for systemic infections caused by herpes virus. In Japan, only topical ACV is permitted for use against herpetic keratitis. We investigated the effectiveness of topical ACV, oral VACV and oral FCV on mouse epithelial herpetic keratitis. METHODS: C57/BL76 mice were inoculated with HSV-1 McKrae strain in the cornea. Once infection was confirmed 4 days after inoculation, topical ACV, oral VACV and FCV were started and administered for 5 days. Control groups were given either topical or oral saline. On days 2, 4, 6 and 10 after medication started, tears, eyeballs, and trigeminal ganglia were examined using viral culture and real-time PCR. RESULTS: Viral culture of tears detected no HSV in the topical ACV group on day 4 after administration start; with similar results for the oral VACV group on day 4; and the oral FCV group on day 6. Real-time PCR of the eyeballs showed significant decrease of HSV DNA copy number in the topical ACV group on days 4 and 6 compared to the topical saline group. Real-time PCR of the trigeminal ganglia showed significant decrease of HSV DNA copy number in the oral VACV group on days 4 and 6, and in the oral FCV group on day 6 compared to the oral saline group. CONCLUSION: We suggest that 5-day administration of topical ACV, oral VACV and oral FCV are effective for mouse epithelial herpetic keratitis and sufficiently decrease HSV amounts in the ocular surface and eyeballs. SN - 1613-2246 UR - https://www.unboundmedicine.com/medline/citation/25707352/Effects_of_antiviral_medications_on_herpetic_epithelial_keratitis_in_mice_ L2 - https://dx.doi.org/10.1007/s10384-015-0375-3 DB - PRIME DP - Unbound Medicine ER -