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Quantitative bioanalysis of paracetamol in rats using volumetric absorptive microsampling (VAMS).
J Pharm Biomed Anal 2015; 108:61-9JP

Abstract

Volumetric absorptive microsampling (VAMS) is a simple intuitive technique for collecting and quantitative analysis of dried blood samples. It enables the collection of an accurate blood volume (approximately 10μL) regardless of blood hematocrit. A bioanalytical method for the determination of paracetamol in dried blood supported on VAMS samplers has been validated and used to support a toxicokinetic (TK) study in rat. The calculated TK parameters were comparable to those obtained from blood-water (1:1, v/v) samples. VAMS is demonstrated to be a robust method that simplifies both the blood sample collection and bioanalytical laboratory procedures and generates high quality quantitative data. However, problems were encountered with controlling the bleed rate during sample collection, resulting in the VAMS tips being flooded and highlighting the need for bleeding methods to be compatible with microsampling techniques to avoid wasting blood. Alternative sample collection procedures are discussed that minimize these issues.

Authors+Show Affiliations

Bioanalytical Science & Toxicokinetics, Drug Metabolism & Pharmacokinetics, GlaxoSmithKline Research & Development, Ware, UK.Bioanalytical Science & Toxicokinetics, Drug Metabolism & Pharmacokinetics, GlaxoSmithKline Research & Development, Ware, UK.Laboratory Animal Science, GlaxoSmithKline Research & Development, Ware, UK.Bioanalytical Science & Toxicokinetics, Drug Metabolism & Pharmacokinetics, GlaxoSmithKline Research & Development, Ware, UK. Electronic address: neil.spooner@gsk.com.

Pub Type(s)

Comparative Study
Journal Article
Validation Studies

Language

eng

PubMed ID

25710904

Citation

Denniff, Philip, et al. "Quantitative Bioanalysis of Paracetamol in Rats Using Volumetric Absorptive Microsampling (VAMS)." Journal of Pharmaceutical and Biomedical Analysis, vol. 108, 2015, pp. 61-9.
Denniff P, Parry S, Dopson W, et al. Quantitative bioanalysis of paracetamol in rats using volumetric absorptive microsampling (VAMS). J Pharm Biomed Anal. 2015;108:61-9.
Denniff, P., Parry, S., Dopson, W., & Spooner, N. (2015). Quantitative bioanalysis of paracetamol in rats using volumetric absorptive microsampling (VAMS). Journal of Pharmaceutical and Biomedical Analysis, 108, pp. 61-9. doi:10.1016/j.jpba.2015.01.052.
Denniff P, et al. Quantitative Bioanalysis of Paracetamol in Rats Using Volumetric Absorptive Microsampling (VAMS). J Pharm Biomed Anal. 2015 Apr 10;108:61-9. PubMed PMID: 25710904.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitative bioanalysis of paracetamol in rats using volumetric absorptive microsampling (VAMS). AU - Denniff,Philip, AU - Parry,Simon, AU - Dopson,Wesley, AU - Spooner,Neil, Y1 - 2015/02/07/ PY - 2014/12/03/received PY - 2015/01/26/revised PY - 2015/01/29/accepted PY - 2015/2/25/entrez PY - 2015/2/25/pubmed PY - 2015/12/15/medline KW - Dried blood KW - Microsampling KW - Paracetamol KW - Validation KW - Volumetric absorptive microsampling SP - 61 EP - 9 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 108 N2 - Volumetric absorptive microsampling (VAMS) is a simple intuitive technique for collecting and quantitative analysis of dried blood samples. It enables the collection of an accurate blood volume (approximately 10μL) regardless of blood hematocrit. A bioanalytical method for the determination of paracetamol in dried blood supported on VAMS samplers has been validated and used to support a toxicokinetic (TK) study in rat. The calculated TK parameters were comparable to those obtained from blood-water (1:1, v/v) samples. VAMS is demonstrated to be a robust method that simplifies both the blood sample collection and bioanalytical laboratory procedures and generates high quality quantitative data. However, problems were encountered with controlling the bleed rate during sample collection, resulting in the VAMS tips being flooded and highlighting the need for bleeding methods to be compatible with microsampling techniques to avoid wasting blood. Alternative sample collection procedures are discussed that minimize these issues. SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/25710904/Quantitative_bioanalysis_of_paracetamol_in_rats_using_volumetric_absorptive_microsampling__VAMS__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(15)00062-X DB - PRIME DP - Unbound Medicine ER -