Tags

Type your tag names separated by a space and hit enter

Budesonide increases TLR4 and TLR2 expression in Treg lymphocytes of allergic asthmatics.
Pulm Pharmacol Ther 2015; 32:93-100PP

Abstract

BACKGROUND

Reduced innate immunity responses as well as reduced T regulatory activities characterise bronchial asthma.

OBJECTIVES

In this study the effect of budesonide on the expression of TLR4 and TLR2 in T regulatory lymphocyte sub-population was assessed.

METHODS

TLR4 and TLR2 expression in total peripheral blood mononuclear cells (PBMC), in CD4+/CD25+ and in CD4+/CD25- was evaluated, by flow cytometric analysis, in mild intermittent asthmatics (n = 14) and in controls (n = 11). The in vitro effects of budesonide in modulating: TLR4 and TLR2 expression in controls and in asthmatics; IL-10 expression and cytokine release (IL-6 and TNF-α selected by a multiplex assay) in asthmatics were also explored.

RESULTS

TLR4 and TLR2 were reduced in total PBMC from asthmatics in comparison to PBMC from controls. CD4+CD25+ cells expressed at higher extent TLR2 and TLR4 in comparison to CD4+CD25- cells. Budesonide was able to increase the expression of TLR4, TLR2 and IL-10 in CD4+/CD25 highly+ cells from asthmatics. TLR4 ligand, LPS induced Foxp3 expression. Budesonide was also able to reduce the release of IL-6 and TNF-α by PBMC of asthmatics.

CONCLUSIONS

Budesonide potentiates the activity of Treg by increasing TLR4, TLR2 and IL-10 expression. This event is associated to the decreased release of IL-6 and TNF-α in PBMC treated with budesonide. These findings shed light on new mechanisms by which corticosteroids, drugs widely used for the clinical management of bronchial asthma, control T lymphocyte activation.

Authors+Show Affiliations

Istituto di Biomedicina e Immunologia Molecolare, Unità di Immunopatologia e Farmacologia Clinica e Sperimentale dell'Apparato Respiratorio, Consiglio Nazionale delle Ricerche, Palermo, Italy. Electronic address: pace@ibim.cnr.it.Istituto di Biomedicina e Immunologia Molecolare, Unità di Immunopatologia e Farmacologia Clinica e Sperimentale dell'Apparato Respiratorio, Consiglio Nazionale delle Ricerche, Palermo, Italy.Istituto di Biomedicina e Immunologia Molecolare, Unità di Immunopatologia e Farmacologia Clinica e Sperimentale dell'Apparato Respiratorio, Consiglio Nazionale delle Ricerche, Palermo, Italy.Istituto di Biomedicina e Immunologia Molecolare, Unità di Immunopatologia e Farmacologia Clinica e Sperimentale dell'Apparato Respiratorio, Consiglio Nazionale delle Ricerche, Palermo, Italy.Department of Dermatology, University of Palermo, Palermo, Italy.Dipartimento Biotecnologie e Neuroscienze Cliniche, Sezione di Otorinolaringoiatria, University of Palermo, Italy.Istituto di Biomedicina e Immunologia Molecolare, Unità di Immunopatologia e Farmacologia Clinica e Sperimentale dell'Apparato Respiratorio, Consiglio Nazionale delle Ricerche, Palermo, Italy.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25722071

Citation

Pace, Elisabetta, et al. "Budesonide Increases TLR4 and TLR2 Expression in Treg Lymphocytes of Allergic Asthmatics." Pulmonary Pharmacology & Therapeutics, vol. 32, 2015, pp. 93-100.
Pace E, Di Sano C, Ferraro M, et al. Budesonide increases TLR4 and TLR2 expression in Treg lymphocytes of allergic asthmatics. Pulm Pharmacol Ther. 2015;32:93-100.
Pace, E., Di Sano, C., Ferraro, M., Bruno, A., Caputo, V., Gallina, S., & Gjomarkaj, M. (2015). Budesonide increases TLR4 and TLR2 expression in Treg lymphocytes of allergic asthmatics. Pulmonary Pharmacology & Therapeutics, 32, pp. 93-100. doi:10.1016/j.pupt.2015.02.003.
Pace E, et al. Budesonide Increases TLR4 and TLR2 Expression in Treg Lymphocytes of Allergic Asthmatics. Pulm Pharmacol Ther. 2015;32:93-100. PubMed PMID: 25722071.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Budesonide increases TLR4 and TLR2 expression in Treg lymphocytes of allergic asthmatics. AU - Pace,Elisabetta, AU - Di Sano,Caterina, AU - Ferraro,Maria, AU - Bruno,Andreina, AU - Caputo,Valentina, AU - Gallina,Salvatore, AU - Gjomarkaj,Mark, Y1 - 2015/02/24/ PY - 2014/10/31/received PY - 2015/01/23/revised PY - 2015/02/13/accepted PY - 2015/2/28/entrez PY - 2015/2/28/pubmed PY - 2016/3/22/medline KW - Corticosteroids KW - Immunoregulation KW - T lymphocytes KW - TLR SP - 93 EP - 100 JF - Pulmonary pharmacology & therapeutics JO - Pulm Pharmacol Ther VL - 32 N2 - BACKGROUND: Reduced innate immunity responses as well as reduced T regulatory activities characterise bronchial asthma. OBJECTIVES: In this study the effect of budesonide on the expression of TLR4 and TLR2 in T regulatory lymphocyte sub-population was assessed. METHODS: TLR4 and TLR2 expression in total peripheral blood mononuclear cells (PBMC), in CD4+/CD25+ and in CD4+/CD25- was evaluated, by flow cytometric analysis, in mild intermittent asthmatics (n = 14) and in controls (n = 11). The in vitro effects of budesonide in modulating: TLR4 and TLR2 expression in controls and in asthmatics; IL-10 expression and cytokine release (IL-6 and TNF-α selected by a multiplex assay) in asthmatics were also explored. RESULTS: TLR4 and TLR2 were reduced in total PBMC from asthmatics in comparison to PBMC from controls. CD4+CD25+ cells expressed at higher extent TLR2 and TLR4 in comparison to CD4+CD25- cells. Budesonide was able to increase the expression of TLR4, TLR2 and IL-10 in CD4+/CD25 highly+ cells from asthmatics. TLR4 ligand, LPS induced Foxp3 expression. Budesonide was also able to reduce the release of IL-6 and TNF-α by PBMC of asthmatics. CONCLUSIONS: Budesonide potentiates the activity of Treg by increasing TLR4, TLR2 and IL-10 expression. This event is associated to the decreased release of IL-6 and TNF-α in PBMC treated with budesonide. These findings shed light on new mechanisms by which corticosteroids, drugs widely used for the clinical management of bronchial asthma, control T lymphocyte activation. SN - 1522-9629 UR - https://www.unboundmedicine.com/medline/citation/25722071/Budesonide_increases_TLR4_and_TLR2_expression_in_Treg_lymphocytes_of_allergic_asthmatics_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1094-5539(15)00022-X DB - PRIME DP - Unbound Medicine ER -