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Paternal obesity in a rodent model affects placental gene expression in a sex-specific manner.
Reproduction. 2015 May; 149(5):435-44.R

Abstract

Fetal growth restriction (FGR) is a major obstetric complication stemming from poor placental development. We have previously demonstrated that paternal obesity in mice is associated with impaired embryo development and significantly reduced fetal and placental weights. We hypothesised that the FGR observed in our rodent model of paternal diet-induced obesity is associated with alterations in metabolic, cell signalling and stress pathways. Male C57BL/6 mice were fed either a normal or high-fat diet for 10 weeks before sperm collection for IVF and subsequent embryo transfer. On embryonic day 14, placentas were collected and RNA extracted from both male and female placentas to assess mRNA expression of 24 target genes using custom RT-qPCR arrays. Peroxisome proliferator-activated receptor alpha (Ppara) and caspase-12 (Casp12) expression were significantly altered in male placentas from obese fathers compared with normal (P<0.05), but not female placentas. PPARA and CASP12 proteins were localised within the placenta to trophoblast giant cells by immunohistochemistry, and relative protein abundance was determined by western blot analysis. DNA was also extracted from the same placentas to determine methylation status. Global DNA methylation was significantly increased in female placentas from obese fathers compared with normal (P<0.05), but not male placentas. In this study, we demonstrate for the first time that paternal obesity is associated with changes in gene expression and methylation status of extraembryonic tissue in a sex-specific manner. These findings reinforce the negative consequences of paternal obesity before conception, and emphasise the need for more lifestyle advice for prospective fathers.

Authors+Show Affiliations

Department of ZoologyUniversity of Melbourne, Parkville, Victoria 3010, AustraliaTranslational Obstetrics GroupDepartment of Obstetrics and Gynaecology, Mercy Hospital for Women, University of Melbourne, Heidelberg, Victoria 3084, Australia Department of ZoologyUniversity of Melbourne, Parkville, Victoria 3010, AustraliaTranslational Obstetrics GroupDepartment of Obstetrics and Gynaecology, Mercy Hospital for Women, University of Melbourne, Heidelberg, Victoria 3084, Australia nkbinder@unimelb.edu.au.Department of ZoologyUniversity of Melbourne, Parkville, Victoria 3010, AustraliaTranslational Obstetrics GroupDepartment of Obstetrics and Gynaecology, Mercy Hospital for Women, University of Melbourne, Heidelberg, Victoria 3084, Australia.Department of ZoologyUniversity of Melbourne, Parkville, Victoria 3010, AustraliaTranslational Obstetrics GroupDepartment of Obstetrics and Gynaecology, Mercy Hospital for Women, University of Melbourne, Heidelberg, Victoria 3084, Australia.Department of ZoologyUniversity of Melbourne, Parkville, Victoria 3010, AustraliaTranslational Obstetrics GroupDepartment of Obstetrics and Gynaecology, Mercy Hospital for Women, University of Melbourne, Heidelberg, Victoria 3084, Australia.Department of ZoologyUniversity of Melbourne, Parkville, Victoria 3010, AustraliaTranslational Obstetrics GroupDepartment of Obstetrics and Gynaecology, Mercy Hospital for Women, University of Melbourne, Heidelberg, Victoria 3084, Australia.Department of ZoologyUniversity of Melbourne, Parkville, Victoria 3010, AustraliaTranslational Obstetrics GroupDepartment of Obstetrics and Gynaecology, Mercy Hospital for Women, University of Melbourne, Heidelberg, Victoria 3084, Australia.

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25725082

Citation

Binder, Natalie K., et al. "Paternal Obesity in a Rodent Model Affects Placental Gene Expression in a Sex-specific Manner." Reproduction (Cambridge, England), vol. 149, no. 5, 2015, pp. 435-44.
Binder NK, Beard SA, Kaitu'u-Lino TJ, et al. Paternal obesity in a rodent model affects placental gene expression in a sex-specific manner. Reproduction. 2015;149(5):435-44.
Binder, N. K., Beard, S. A., Kaitu'u-Lino, T. J., Tong, S., Hannan, N. J., & Gardner, D. K. (2015). Paternal obesity in a rodent model affects placental gene expression in a sex-specific manner. Reproduction (Cambridge, England), 149(5), 435-44. https://doi.org/10.1530/REP-14-0676
Binder NK, et al. Paternal Obesity in a Rodent Model Affects Placental Gene Expression in a Sex-specific Manner. Reproduction. 2015;149(5):435-44. PubMed PMID: 25725082.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Paternal obesity in a rodent model affects placental gene expression in a sex-specific manner. AU - Binder,Natalie K, AU - Beard,Sally A, AU - Kaitu'u-Lino,Tu'uhevaha J, AU - Tong,Stephen, AU - Hannan,Natalie J, AU - Gardner,David K, Y1 - 2015/02/27/ PY - 2015/3/1/entrez PY - 2015/3/1/pubmed PY - 2016/1/13/medline SP - 435 EP - 44 JF - Reproduction (Cambridge, England) JO - Reproduction VL - 149 IS - 5 N2 - Fetal growth restriction (FGR) is a major obstetric complication stemming from poor placental development. We have previously demonstrated that paternal obesity in mice is associated with impaired embryo development and significantly reduced fetal and placental weights. We hypothesised that the FGR observed in our rodent model of paternal diet-induced obesity is associated with alterations in metabolic, cell signalling and stress pathways. Male C57BL/6 mice were fed either a normal or high-fat diet for 10 weeks before sperm collection for IVF and subsequent embryo transfer. On embryonic day 14, placentas were collected and RNA extracted from both male and female placentas to assess mRNA expression of 24 target genes using custom RT-qPCR arrays. Peroxisome proliferator-activated receptor alpha (Ppara) and caspase-12 (Casp12) expression were significantly altered in male placentas from obese fathers compared with normal (P<0.05), but not female placentas. PPARA and CASP12 proteins were localised within the placenta to trophoblast giant cells by immunohistochemistry, and relative protein abundance was determined by western blot analysis. DNA was also extracted from the same placentas to determine methylation status. Global DNA methylation was significantly increased in female placentas from obese fathers compared with normal (P<0.05), but not male placentas. In this study, we demonstrate for the first time that paternal obesity is associated with changes in gene expression and methylation status of extraembryonic tissue in a sex-specific manner. These findings reinforce the negative consequences of paternal obesity before conception, and emphasise the need for more lifestyle advice for prospective fathers. SN - 1741-7899 UR - https://www.unboundmedicine.com/medline/citation/25725082/Paternal_obesity_in_a_rodent_model_affects_placental_gene_expression_in_a_sex_specific_manner_ L2 - https://rep.bioscientifica.com/doi/10.1530/REP-14-0676 DB - PRIME DP - Unbound Medicine ER -