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Joint-dependent response to impact and implications for post-traumatic osteoarthritis.
Osteoarthritis Cartilage. 2015 Jul; 23(7):1130-7.OC

Abstract

OBJECTIVE

The prevalence of osteoarthritis (OA) varies between joints. Cartilage in eight different joints was evaluated to elucidate the disparate susceptibilities between joints to post-traumatic OA (PTOA) and provide evidence for joint-specific clinical treatments. The hypothesis was that cartilage in different joints would have varying cell death and anabolic gene expression profiles after injury.

METHODS

Adult equine cartilage explants were harvested from shoulder (SH), elbow (EL), carpal (CA), metacarpophalangeal (MC), patellofemoral (FP), tarsal (TA), metatarsophalangeal (MT), and proximal interphalangeal (PP) joints, and injured by loading with 30 MPa within 1 s. Fractional dissipated energy, cell density, cell death, and gene expression were quantified.

RESULTS

PP had the highest fractional dissipated energy (94%, 95% confidence interval [CI] 88 to 101%). Cell density was highest in the superficial zone in all samples, with MC and MT having the highest peak density. Injured samples had significantly increased cell death (13.5%, 95% CI 9.1 to 17.9%) than non-injured samples (6.8%, 95% CI 2.5 to 11.1%, P = 0.016); however, cell death after injury was not significantly different between joints. Gene expression was significantly different between joints. CD-RAP expression in normal cartilage was lowest in FP (Cp = 21, 95% CI -80 to 122). After injury, the change in CD-RAP expression increased and was highest in FP (147% relative increase after injury, 95% CI 64 to 213).

CONCLUSION

Different joints have different baseline characteristics, including cell density and gene expression, and responses to injury, including energy dissipation and gene expression. These unique characteristics may explain differences in OA prevalence and suggest differences in susceptibility to PTOA.

CLINICAL RELEVANCE

Understanding differences in the response to injury and potential susceptibility to OA can lead to the development of preventative or treatment strategies.

KEY TERMS

Gene expression, cartilage injury, chondrocyte, multiphoton microscopy, cartilage biomechanical properties, PTOA.

WHAT IS KNOWN ABOUT THE SUBJECT

The prevalence of OA is variable among joints; however, most laboratory studies are performed on a single joint - most commonly the knee, and extrapolated to other joints such as the ankle or shoulder. A small number of studies have compared knee and ankle cartilage and reported differences in mechanical properties and gene expression.

WHAT THIS STUDY ADDS TO EXISTING KNOWLEDGE

There are differences in baseline cell density and gene expression, and differences in response to injury, including gene expression and cell death. This suggests that there are inherent differences leading to varying susceptibilities in OA prevalence among joints. Joint-specific treatments may improve OA therapies.

Authors+Show Affiliations

Department of Clinical Sciences, Cornell University, Ithaca, NY, USA.Department of Clinical Veterinary and Animal Science, University of Copenhagen, København, Denmark.Department of Comparative Biomedicine and Food Science, University of Padova, Padova, Italy.Department of Biomedical Engineering, Cornell University, Ithaca, NY, USA.Department of Clinical Sciences, Cornell University, Ithaca, NY, USA.Department of Biomedical Engineering, Cornell University, Ithaca, NY, USA.Department of Clinical Sciences, Cornell University, Ithaca, NY, USA. Electronic address: laf4@cornell.edu.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25725390

Citation

Novakofski, K D., et al. "Joint-dependent Response to Impact and Implications for Post-traumatic Osteoarthritis." Osteoarthritis and Cartilage, vol. 23, no. 7, 2015, pp. 1130-7.
Novakofski KD, Berg LC, Bronzini I, et al. Joint-dependent response to impact and implications for post-traumatic osteoarthritis. Osteoarthr Cartil. 2015;23(7):1130-7.
Novakofski, K. D., Berg, L. C., Bronzini, I., Bonnevie, E. D., Poland, S. G., Bonassar, L. J., & Fortier, L. A. (2015). Joint-dependent response to impact and implications for post-traumatic osteoarthritis. Osteoarthritis and Cartilage, 23(7), 1130-7. https://doi.org/10.1016/j.joca.2015.02.023
Novakofski KD, et al. Joint-dependent Response to Impact and Implications for Post-traumatic Osteoarthritis. Osteoarthr Cartil. 2015;23(7):1130-7. PubMed PMID: 25725390.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Joint-dependent response to impact and implications for post-traumatic osteoarthritis. AU - Novakofski,K D, AU - Berg,L C, AU - Bronzini,I, AU - Bonnevie,E D, AU - Poland,S G, AU - Bonassar,L J, AU - Fortier,L A, Y1 - 2015/02/26/ PY - 2014/08/26/received PY - 2015/02/13/revised PY - 2015/02/18/accepted PY - 2015/3/1/entrez PY - 2015/3/1/pubmed PY - 2016/3/16/medline KW - Cartilage biomechanical properties KW - Cartilage injury KW - Chondrocyte KW - Gene expression KW - Multiphoton microscopy KW - PTOA SP - 1130 EP - 7 JF - Osteoarthritis and cartilage JO - Osteoarthr. Cartil. VL - 23 IS - 7 N2 - OBJECTIVE: The prevalence of osteoarthritis (OA) varies between joints. Cartilage in eight different joints was evaluated to elucidate the disparate susceptibilities between joints to post-traumatic OA (PTOA) and provide evidence for joint-specific clinical treatments. The hypothesis was that cartilage in different joints would have varying cell death and anabolic gene expression profiles after injury. METHODS: Adult equine cartilage explants were harvested from shoulder (SH), elbow (EL), carpal (CA), metacarpophalangeal (MC), patellofemoral (FP), tarsal (TA), metatarsophalangeal (MT), and proximal interphalangeal (PP) joints, and injured by loading with 30 MPa within 1 s. Fractional dissipated energy, cell density, cell death, and gene expression were quantified. RESULTS: PP had the highest fractional dissipated energy (94%, 95% confidence interval [CI] 88 to 101%). Cell density was highest in the superficial zone in all samples, with MC and MT having the highest peak density. Injured samples had significantly increased cell death (13.5%, 95% CI 9.1 to 17.9%) than non-injured samples (6.8%, 95% CI 2.5 to 11.1%, P = 0.016); however, cell death after injury was not significantly different between joints. Gene expression was significantly different between joints. CD-RAP expression in normal cartilage was lowest in FP (Cp = 21, 95% CI -80 to 122). After injury, the change in CD-RAP expression increased and was highest in FP (147% relative increase after injury, 95% CI 64 to 213). CONCLUSION: Different joints have different baseline characteristics, including cell density and gene expression, and responses to injury, including energy dissipation and gene expression. These unique characteristics may explain differences in OA prevalence and suggest differences in susceptibility to PTOA. CLINICAL RELEVANCE: Understanding differences in the response to injury and potential susceptibility to OA can lead to the development of preventative or treatment strategies. KEY TERMS: Gene expression, cartilage injury, chondrocyte, multiphoton microscopy, cartilage biomechanical properties, PTOA. WHAT IS KNOWN ABOUT THE SUBJECT: The prevalence of OA is variable among joints; however, most laboratory studies are performed on a single joint - most commonly the knee, and extrapolated to other joints such as the ankle or shoulder. A small number of studies have compared knee and ankle cartilage and reported differences in mechanical properties and gene expression. WHAT THIS STUDY ADDS TO EXISTING KNOWLEDGE: There are differences in baseline cell density and gene expression, and differences in response to injury, including gene expression and cell death. This suggests that there are inherent differences leading to varying susceptibilities in OA prevalence among joints. Joint-specific treatments may improve OA therapies. SN - 1522-9653 UR - https://www.unboundmedicine.com/medline/citation/25725390/Joint_dependent_response_to_impact_and_implications_for_post_traumatic_osteoarthritis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1063-4584(15)00067-9 DB - PRIME DP - Unbound Medicine ER -