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Oxidation of H2S in mammalian cells and mitochondria.
Methods Enzymol. 2015; 554:201-28.ME

Abstract

Hydrogen sulfide (H2S) is the third gasotransmitter described in mammals. These gasotransmitters (H2S, CO, and NO) are small molecules able to diffuse freely across membranes and thus susceptible to reach easily intracellular targets, one of which is the respiratory enzyme cytochrome oxidase subject to complete inhibition by low micromolar concentrations of these gases. However in contrast to NO or CO, H2S can be metabolized by a sulfide quinone reductase feeding the mitochondrial respiratory chain with the hydrogen atoms of sulfide. Sulfide is thus a two-sided molecule: substrate or poison according to the concentration. The aim of this chapter is to present a mean to monitor sulfide oxidation by isolated mitochondria or cells and to summarize how the properties of this amazing couple (mitochondria and sulfide) translate into practical and conceptual consequences.

Authors+Show Affiliations

Inserm U1016, Institut Cochin, Paris, France; CNRS UM8104, Institut Cochin, Paris, France; Université Paris Descartes UMR-S1016, Institut Cochin, Paris, France.Inserm U1016, Institut Cochin, Paris, France; CNRS UM8104, Institut Cochin, Paris, France; Université Paris Descartes UMR-S1016, Institut Cochin, Paris, France.Inserm U1016, Institut Cochin, Paris, France; CNRS UM8104, Institut Cochin, Paris, France; Université Paris Descartes UMR-S1016, Institut Cochin, Paris, France.INRA-CRNH-IdF-AgroParisTech, UMR 914 Nutrition Physiology and Ingestive Behavior, Paris, France.INRA-CRNH-IdF-AgroParisTech, UMR 914 Nutrition Physiology and Ingestive Behavior, Paris, France.Inserm U1016, Institut Cochin, Paris, France; CNRS UM8104, Institut Cochin, Paris, France; Université Paris Descartes UMR-S1016, Institut Cochin, Paris, France. Electronic address: frederic.bouillaud@inserm.fr.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25725524

Citation

Abou-Hamdan, Abbas, et al. "Oxidation of H2S in Mammalian Cells and Mitochondria." Methods in Enzymology, vol. 554, 2015, pp. 201-28.
Abou-Hamdan A, Guedouari-Bounihi H, Lenoir V, et al. Oxidation of H2S in mammalian cells and mitochondria. Methods Enzymol. 2015;554:201-28.
Abou-Hamdan, A., Guedouari-Bounihi, H., Lenoir, V., Andriamihaja, M., Blachier, F., & Bouillaud, F. (2015). Oxidation of H2S in mammalian cells and mitochondria. Methods in Enzymology, 554, 201-28. https://doi.org/10.1016/bs.mie.2014.11.042
Abou-Hamdan A, et al. Oxidation of H2S in Mammalian Cells and Mitochondria. Methods Enzymol. 2015;554:201-28. PubMed PMID: 25725524.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oxidation of H2S in mammalian cells and mitochondria. AU - Abou-Hamdan,Abbas, AU - Guedouari-Bounihi,Hala, AU - Lenoir,Véronique, AU - Andriamihaja,Mireille, AU - Blachier,François, AU - Bouillaud,Frédéric, Y1 - 2015/01/22/ PY - 2015/3/2/entrez PY - 2015/3/3/pubmed PY - 2015/12/15/medline KW - Bioenergetics KW - Hydrogen sulfide KW - Metabolism KW - Mitochondria KW - Oxygen KW - Respiration KW - Sulfur SP - 201 EP - 28 JF - Methods in enzymology JO - Methods Enzymol VL - 554 N2 - Hydrogen sulfide (H2S) is the third gasotransmitter described in mammals. These gasotransmitters (H2S, CO, and NO) are small molecules able to diffuse freely across membranes and thus susceptible to reach easily intracellular targets, one of which is the respiratory enzyme cytochrome oxidase subject to complete inhibition by low micromolar concentrations of these gases. However in contrast to NO or CO, H2S can be metabolized by a sulfide quinone reductase feeding the mitochondrial respiratory chain with the hydrogen atoms of sulfide. Sulfide is thus a two-sided molecule: substrate or poison according to the concentration. The aim of this chapter is to present a mean to monitor sulfide oxidation by isolated mitochondria or cells and to summarize how the properties of this amazing couple (mitochondria and sulfide) translate into practical and conceptual consequences. SN - 1557-7988 UR - https://www.unboundmedicine.com/medline/citation/25725524/Oxidation_of_H2S_in_mammalian_cells_and_mitochondria_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0076-6879(14)00107-4 DB - PRIME DP - Unbound Medicine ER -