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IL-7 promotes thymocyte proliferation and maintains immunocompetent thymocytes bearing alpha beta or gamma delta T-cell receptors in vitro: synergism with IL-2.
J Immunol. 1989 Nov 01; 143(9):2917-22.JI

Abstract

IL-7 induced the proliferation of normal thymocytes and the effect was synergistically potentiated by a small dose of IL-2, which by itself hardly affected thymocyte proliferation. No synergism was observed between IL-7 and any one of the other lymphokines including IL-1, IL-3, and IL-4. The thymocyte culture stimulated with IL-7 and IL-2 consisted of single positive (CD4+CD8- and CD4-CD8+) and double negative (CD4-CD8-) populations, and double positive (CD4+CD8+) cells were completely deleted. Both single positive and double negative thymocytes expressed CD3, but only the former exhibited V beta 8 and V beta 6 in an expected proportion (approximately 30% in BALB/c mice) and the latter none at all. Immunoprecipitation of the cultured thymocytes by anti-TCR gamma antibody, on the other hand, revealed the presence of a TCR gamma chain. Taken together, these results indicated that the thymocyte cultured with IL-7 and IL-2 consisted of mature T cells bearing alpha beta or gamma delta TCR. Experiments using preselected thymocyte subpopulations indicated that double negative cells responded to both IL-7 and IL-2 with positive synergism when combined, while thymocytes enriched for single positive cells preferentially responded to IL-7 with little response to IL-2 and no detectable synergism. Double positive thymocytes showed no proliferation in response to IL-7 and IL-2. In contrast to single positive thymocytes, splenic T cells hardly responded to IL-7, although significant proliferation was induced in the presence of a low dose of IL-2. Thymocytes cultured with IL-7 and IL-2 showed little nonspecific cytotoxic activity, but responded to Con A or alloantigen, whereas those stimulated with a high dose of IL-2 alone exhibited potent cytotoxic activity. These results indicated that IL-7 was involved in the generation of immunocompetent T cells in the thymus in concert with IL-2.

Authors+Show Affiliations

Department of Medicine, Jichi Medical School, Tochigi, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2572646

Citation

Okazaki, H, et al. "IL-7 Promotes Thymocyte Proliferation and Maintains Immunocompetent Thymocytes Bearing Alpha Beta or Gamma Delta T-cell Receptors in Vitro: Synergism With IL-2." Journal of Immunology (Baltimore, Md. : 1950), vol. 143, no. 9, 1989, pp. 2917-22.
Okazaki H, Ito M, Sudo T, et al. IL-7 promotes thymocyte proliferation and maintains immunocompetent thymocytes bearing alpha beta or gamma delta T-cell receptors in vitro: synergism with IL-2. J Immunol. 1989;143(9):2917-22.
Okazaki, H., Ito, M., Sudo, T., Hattori, M., Kano, S., Katsura, Y., & Minato, N. (1989). IL-7 promotes thymocyte proliferation and maintains immunocompetent thymocytes bearing alpha beta or gamma delta T-cell receptors in vitro: synergism with IL-2. Journal of Immunology (Baltimore, Md. : 1950), 143(9), 2917-22.
Okazaki H, et al. IL-7 Promotes Thymocyte Proliferation and Maintains Immunocompetent Thymocytes Bearing Alpha Beta or Gamma Delta T-cell Receptors in Vitro: Synergism With IL-2. J Immunol. 1989 Nov 1;143(9):2917-22. PubMed PMID: 2572646.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - IL-7 promotes thymocyte proliferation and maintains immunocompetent thymocytes bearing alpha beta or gamma delta T-cell receptors in vitro: synergism with IL-2. AU - Okazaki,H, AU - Ito,M, AU - Sudo,T, AU - Hattori,M, AU - Kano,S, AU - Katsura,Y, AU - Minato,N, PY - 1989/11/1/pubmed PY - 1989/11/1/medline PY - 1989/11/1/entrez SP - 2917 EP - 22 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J Immunol VL - 143 IS - 9 N2 - IL-7 induced the proliferation of normal thymocytes and the effect was synergistically potentiated by a small dose of IL-2, which by itself hardly affected thymocyte proliferation. No synergism was observed between IL-7 and any one of the other lymphokines including IL-1, IL-3, and IL-4. The thymocyte culture stimulated with IL-7 and IL-2 consisted of single positive (CD4+CD8- and CD4-CD8+) and double negative (CD4-CD8-) populations, and double positive (CD4+CD8+) cells were completely deleted. Both single positive and double negative thymocytes expressed CD3, but only the former exhibited V beta 8 and V beta 6 in an expected proportion (approximately 30% in BALB/c mice) and the latter none at all. Immunoprecipitation of the cultured thymocytes by anti-TCR gamma antibody, on the other hand, revealed the presence of a TCR gamma chain. Taken together, these results indicated that the thymocyte cultured with IL-7 and IL-2 consisted of mature T cells bearing alpha beta or gamma delta TCR. Experiments using preselected thymocyte subpopulations indicated that double negative cells responded to both IL-7 and IL-2 with positive synergism when combined, while thymocytes enriched for single positive cells preferentially responded to IL-7 with little response to IL-2 and no detectable synergism. Double positive thymocytes showed no proliferation in response to IL-7 and IL-2. In contrast to single positive thymocytes, splenic T cells hardly responded to IL-7, although significant proliferation was induced in the presence of a low dose of IL-2. Thymocytes cultured with IL-7 and IL-2 showed little nonspecific cytotoxic activity, but responded to Con A or alloantigen, whereas those stimulated with a high dose of IL-2 alone exhibited potent cytotoxic activity. These results indicated that IL-7 was involved in the generation of immunocompetent T cells in the thymus in concert with IL-2. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/2572646/IL_7_promotes_thymocyte_proliferation_and_maintains_immunocompetent_thymocytes_bearing_alpha_beta_or_gamma_delta_T_cell_receptors_in_vitro:_synergism_with_IL_2_ L2 - https://www.jimmunol.org/lookup/pmidlookup?view=long&pmid=2572646 DB - PRIME DP - Unbound Medicine ER -