Tags

Type your tag names separated by a space and hit enter

Molecular characterization of hepatitis C virus genotype 6 subtypes in Thai blood donors.
J Microbiol Immunol Infect. 2017 Feb; 50(1):26-31.JM

Abstract

BACKGROUND

Hepatitis C virus (HCV) genotype is important for identifying effective antiviral therapy, evaluating pathogenic severity, and tracking transmission routes. In Thailand, HCV genotypes 3 and 1 are the most common. We have previously demonstrated an increasing appearance of genotype 6 in HCV infections in Thailand. However, only limited epidemiological data on genotype 6 in Thailand are available. This study aimed to characterize HCV genotype 6 among apparently healthy Thai blood donors.

METHODS

In total, 240 blood samples were collected from Phitsanulok Regional Blood Center, Phitsanulok, Thailand. RNA was reverse transcribed and amplified by the nested polymerase chain reaction. HCV genotyping was performed by direct sequencing and phylogenetic tree analysis of core sequences. Amino acid polymorphism of various subtypes of HCV genotype 6 was investigated.

RESULTS

Of the 240 samples, 192 were successfully sequenced for the core region and 84 were determined to be of HCV genotype 6 by phylogenetic analysis. The most prevalent HCV-6 subtypes were 6f > 6n > 6c > 6i. Amino acid sequences of the partial core region among these four subtypes differed by one to seven residues.

CONCLUSION

For HCV-6, the subtype 6f was commonly found in Thai blood donors. Comparison of core protein from various HCV-6 subtypes showed substantial polymorphisms, which may form the basis of future studies using samples from patients with clear HCV histories. This feature can be applied to therapies tailored to particular genotype variants.

Authors+Show Affiliations

Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand. Electronic address: sisaya@rocketmail.com.Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25731701

Citation

Sistayanarain, Anchalee, and Suriya Chaiwong. "Molecular Characterization of Hepatitis C Virus Genotype 6 Subtypes in Thai Blood Donors." Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi, vol. 50, no. 1, 2017, pp. 26-31.
Sistayanarain A, Chaiwong S. Molecular characterization of hepatitis C virus genotype 6 subtypes in Thai blood donors. J Microbiol Immunol Infect. 2017;50(1):26-31.
Sistayanarain, A., & Chaiwong, S. (2017). Molecular characterization of hepatitis C virus genotype 6 subtypes in Thai blood donors. Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi, 50(1), 26-31. https://doi.org/10.1016/j.jmii.2015.01.006
Sistayanarain A, Chaiwong S. Molecular Characterization of Hepatitis C Virus Genotype 6 Subtypes in Thai Blood Donors. J Microbiol Immunol Infect. 2017;50(1):26-31. PubMed PMID: 25731701.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterization of hepatitis C virus genotype 6 subtypes in Thai blood donors. AU - Sistayanarain,Anchalee, AU - Chaiwong,Suriya, Y1 - 2015/01/30/ PY - 2014/04/24/received PY - 2015/01/02/revised PY - 2015/01/19/accepted PY - 2015/3/4/pubmed PY - 2017/3/23/medline PY - 2015/3/4/entrez KW - Blood donors KW - Genotype and subtype KW - Hepatitis C virus KW - Hepatitis C virus-6 subtypes SP - 26 EP - 31 JF - Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi JO - J Microbiol Immunol Infect VL - 50 IS - 1 N2 - BACKGROUND: Hepatitis C virus (HCV) genotype is important for identifying effective antiviral therapy, evaluating pathogenic severity, and tracking transmission routes. In Thailand, HCV genotypes 3 and 1 are the most common. We have previously demonstrated an increasing appearance of genotype 6 in HCV infections in Thailand. However, only limited epidemiological data on genotype 6 in Thailand are available. This study aimed to characterize HCV genotype 6 among apparently healthy Thai blood donors. METHODS: In total, 240 blood samples were collected from Phitsanulok Regional Blood Center, Phitsanulok, Thailand. RNA was reverse transcribed and amplified by the nested polymerase chain reaction. HCV genotyping was performed by direct sequencing and phylogenetic tree analysis of core sequences. Amino acid polymorphism of various subtypes of HCV genotype 6 was investigated. RESULTS: Of the 240 samples, 192 were successfully sequenced for the core region and 84 were determined to be of HCV genotype 6 by phylogenetic analysis. The most prevalent HCV-6 subtypes were 6f > 6n > 6c > 6i. Amino acid sequences of the partial core region among these four subtypes differed by one to seven residues. CONCLUSION: For HCV-6, the subtype 6f was commonly found in Thai blood donors. Comparison of core protein from various HCV-6 subtypes showed substantial polymorphisms, which may form the basis of future studies using samples from patients with clear HCV histories. This feature can be applied to therapies tailored to particular genotype variants. SN - 1995-9133 UR - https://www.unboundmedicine.com/medline/citation/25731701/Molecular_characterization_of_hepatitis_C_virus_genotype_6_subtypes_in_Thai_blood_donors_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1684-1182(15)00030-4 DB - PRIME DP - Unbound Medicine ER -