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Micro-trichome as a class I homeodomain-leucine zipper gene regulates multicellular trichome development in Cucumis sativus.
J Integr Plant Biol. 2015 Nov; 57(11):925-35.JI

Abstract

Plant trichomes serve as a highly suitable model for investigating cell differentiation at the single-cell level. The regulatory genes involved in unicellular trichome development in Arabidopsis thaliana have been intensively studied, but genes regulating multicellular trichome development in plants remain unclear. Here, we characterized Cucumis sativus (cucumber) trichomes as representative multicellular and unbranched structures, and identified Micro-trichome (Mict), using map-based cloning in an F2 segregating population of 7,936 individuals generated from a spontaneous mict mutant. In mict plants, trichomes in both leaves and fruits, are small, poorly developed, and denser than in the wild type. Sequence analysis revealed that a 2,649-bp genomic deletion, spanning the first and second exons, occurred in a plant-specific class I homeodomain-leucine zipper gene. Tissue-specific expression analysis indicated that Mict is strongly expressed in the trichome cells. Transcriptome profiling identified potential targets of Mict including putative homologs of genes known in other systems to regulate trichome development, meristem determinacy, and hormone responsiveness. Phylogenic analysis charted the relationships among putative homologs in angiosperms. Our paper represents initial steps toward understanding the development of multicellular trichomes.

Authors+Show Affiliations

School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25735194

Citation

Zhao, Jun-Long, et al. "Micro-trichome as a Class I Homeodomain-leucine Zipper Gene Regulates Multicellular Trichome Development in Cucumis Sativus." Journal of Integrative Plant Biology, vol. 57, no. 11, 2015, pp. 925-35.
Zhao JL, Pan JS, Guan Y, et al. Micro-trichome as a class I homeodomain-leucine zipper gene regulates multicellular trichome development in Cucumis sativus. J Integr Plant Biol. 2015;57(11):925-35.
Zhao, J. L., Pan, J. S., Guan, Y., Zhang, W. W., Bie, B. B., Wang, Y. L., He, H. L., Lian, H. L., & Cai, R. (2015). Micro-trichome as a class I homeodomain-leucine zipper gene regulates multicellular trichome development in Cucumis sativus. Journal of Integrative Plant Biology, 57(11), 925-35. https://doi.org/10.1111/jipb.12345
Zhao JL, et al. Micro-trichome as a Class I Homeodomain-leucine Zipper Gene Regulates Multicellular Trichome Development in Cucumis Sativus. J Integr Plant Biol. 2015;57(11):925-35. PubMed PMID: 25735194.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Micro-trichome as a class I homeodomain-leucine zipper gene regulates multicellular trichome development in Cucumis sativus. AU - Zhao,Jun-Long, AU - Pan,Jun-Song, AU - Guan,Yuan, AU - Zhang,Wei-Wei, AU - Bie,Bei-Bei, AU - Wang,Yun-Li, AU - He,Huan-Le, AU - Lian,Hong-Li, AU - Cai,Run, Y1 - 2015/04/24/ PY - 2014/10/27/received PY - 2015/02/27/accepted PY - 2015/3/5/entrez PY - 2015/3/5/pubmed PY - 2016/9/8/medline KW - Cucumis sativus KW - HD-Zip gene KW - map-based cloning KW - multicellular trichome KW - transcriptome SP - 925 EP - 35 JF - Journal of integrative plant biology JO - J Integr Plant Biol VL - 57 IS - 11 N2 - Plant trichomes serve as a highly suitable model for investigating cell differentiation at the single-cell level. The regulatory genes involved in unicellular trichome development in Arabidopsis thaliana have been intensively studied, but genes regulating multicellular trichome development in plants remain unclear. Here, we characterized Cucumis sativus (cucumber) trichomes as representative multicellular and unbranched structures, and identified Micro-trichome (Mict), using map-based cloning in an F2 segregating population of 7,936 individuals generated from a spontaneous mict mutant. In mict plants, trichomes in both leaves and fruits, are small, poorly developed, and denser than in the wild type. Sequence analysis revealed that a 2,649-bp genomic deletion, spanning the first and second exons, occurred in a plant-specific class I homeodomain-leucine zipper gene. Tissue-specific expression analysis indicated that Mict is strongly expressed in the trichome cells. Transcriptome profiling identified potential targets of Mict including putative homologs of genes known in other systems to regulate trichome development, meristem determinacy, and hormone responsiveness. Phylogenic analysis charted the relationships among putative homologs in angiosperms. Our paper represents initial steps toward understanding the development of multicellular trichomes. SN - 1744-7909 UR - https://www.unboundmedicine.com/medline/citation/25735194/Micro_trichome_as_a_class_I_homeodomain_leucine_zipper_gene_regulates_multicellular_trichome_development_in_Cucumis_sativus_ L2 - https://doi.org/10.1111/jipb.12345 DB - PRIME DP - Unbound Medicine ER -