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Coffee extracts suppress tryptophan breakdown in mitogen-stimulated peripheral blood mononuclear cells.
J Am Coll Nutr. 2015; 34(3):212-23.JA

Abstract

OBJECTIVES

Coffee consumption is considered to exert an influence on mood, the immune system, cardiovascular disease, and cancer development, but the mechanisms of action of coffee and its compounds are only partly known and understood.

METHODS

Immunomodulatory effects of filtered extracts of coffee and decaffeinated coffee as well as coffee compounds were investigated in human peripheral blood mononuclear cells (PBMCs) stimulated with mitogen phytohemagglutinin (PHA). The activation of PBMCs was monitored by the breakdown of tryptophan to kynurenine via enzyme indoleamine 2,3-dioxygenase (IDO) and the production of the immune activation marker neopterin by GTP-cyclohydrolase I (GCH1). Both of these biochemical pathways are induced during cellular immune activation in response to the Th1-type cytokine interferon-γ (IFN-γ).

RESULTS

Filtered extracts of coffee and decaffeinated coffee both suppressed tryptophan breakdown and neopterin formation in mitogen-stimulated PBMCs efficiently and in a dose-dependent manner. Of 4 coffee compounds tested individually, only gallic acid and less strong also caffeic acid had a consistent suppressive influence but also affected cell viability, whereas pure caffeine and chlorogenic acid exerted no relevant effect in the PBMC assay.

CONCLUSION

The parallel influence of extracts on tryptophan breakdown and neopterin production shows an anti-inflammatory and immunosuppressive property of coffee extracts and some of its compounds. When extrapolating the in vitro results to in vivo, IFN-γ-mediated breakdown of tryptophan could be counteracted by the consumption of coffee or decaffeinated coffee. This may increase tryptophan availability for the biosynthesis of the neurotransmitter 5-hydroxytryptamine (serotonin) and thereby improve mood and quality of life.

Authors+Show Affiliations

a Division of Biological Chemistry , Biocenter , Medical University of Innsbruck , Innsbruck , AUSTRIA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25738401

Citation

Gostner, Johanna M., et al. "Coffee Extracts Suppress Tryptophan Breakdown in Mitogen-stimulated Peripheral Blood Mononuclear Cells." Journal of the American College of Nutrition, vol. 34, no. 3, 2015, pp. 212-23.
Gostner JM, Schroecksnadel S, Jenny M, et al. Coffee extracts suppress tryptophan breakdown in mitogen-stimulated peripheral blood mononuclear cells. J Am Coll Nutr. 2015;34(3):212-23.
Gostner, J. M., Schroecksnadel, S., Jenny, M., Klein, A., Ueberall, F., Schennach, H., & Fuchs, D. (2015). Coffee extracts suppress tryptophan breakdown in mitogen-stimulated peripheral blood mononuclear cells. Journal of the American College of Nutrition, 34(3), 212-23. https://doi.org/10.1080/07315724.2014.907756
Gostner JM, et al. Coffee Extracts Suppress Tryptophan Breakdown in Mitogen-stimulated Peripheral Blood Mononuclear Cells. J Am Coll Nutr. 2015;34(3):212-23. PubMed PMID: 25738401.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Coffee extracts suppress tryptophan breakdown in mitogen-stimulated peripheral blood mononuclear cells. AU - Gostner,Johanna M, AU - Schroecksnadel,Sebastian, AU - Jenny,Marcel, AU - Klein,Angela, AU - Ueberall,Florian, AU - Schennach,Harald, AU - Fuchs,Dietmar, Y1 - 2015/03/04/ PY - 2015/3/5/entrez PY - 2015/3/5/pubmed PY - 2016/2/5/medline KW - 3-dioxygenase KW - Abbreviations: CTB = cell titer blue KW - FCS = fetal calf serum KW - GCH1 = GTP-cyclohydrolase I KW - IDO = indoleamine 2,3-dioxygenase KW - IFN = interferon KW - Kyn = kynurenine KW - Kyn/Trp = kynurenine to tryptophan ratio KW - ORAC = oxygen radical absorbance capacity assay KW - PBMC = peripheral blood mononuclear cells KW - PHA = phytohaemagglutinin KW - TE = trolox equivalent KW - Trp = tryptophan KW - coffee KW - indoleamine 2 KW - neopterin KW - peripheral blood mononuclear cells KW - tryptophan SP - 212 EP - 23 JF - Journal of the American College of Nutrition JO - J Am Coll Nutr VL - 34 IS - 3 N2 - OBJECTIVES: Coffee consumption is considered to exert an influence on mood, the immune system, cardiovascular disease, and cancer development, but the mechanisms of action of coffee and its compounds are only partly known and understood. METHODS: Immunomodulatory effects of filtered extracts of coffee and decaffeinated coffee as well as coffee compounds were investigated in human peripheral blood mononuclear cells (PBMCs) stimulated with mitogen phytohemagglutinin (PHA). The activation of PBMCs was monitored by the breakdown of tryptophan to kynurenine via enzyme indoleamine 2,3-dioxygenase (IDO) and the production of the immune activation marker neopterin by GTP-cyclohydrolase I (GCH1). Both of these biochemical pathways are induced during cellular immune activation in response to the Th1-type cytokine interferon-γ (IFN-γ). RESULTS: Filtered extracts of coffee and decaffeinated coffee both suppressed tryptophan breakdown and neopterin formation in mitogen-stimulated PBMCs efficiently and in a dose-dependent manner. Of 4 coffee compounds tested individually, only gallic acid and less strong also caffeic acid had a consistent suppressive influence but also affected cell viability, whereas pure caffeine and chlorogenic acid exerted no relevant effect in the PBMC assay. CONCLUSION: The parallel influence of extracts on tryptophan breakdown and neopterin production shows an anti-inflammatory and immunosuppressive property of coffee extracts and some of its compounds. When extrapolating the in vitro results to in vivo, IFN-γ-mediated breakdown of tryptophan could be counteracted by the consumption of coffee or decaffeinated coffee. This may increase tryptophan availability for the biosynthesis of the neurotransmitter 5-hydroxytryptamine (serotonin) and thereby improve mood and quality of life. SN - 1541-1087 UR - https://www.unboundmedicine.com/medline/citation/25738401/Coffee_extracts_suppress_tryptophan_breakdown_in_mitogen_stimulated_peripheral_blood_mononuclear_cells_ L2 - http://www.tandfonline.com/doi/full/10.1080/07315724.2014.907756 DB - PRIME DP - Unbound Medicine ER -