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Effects of serum from patients with early-onset pre-eclampsia, HELLP syndrome, and antiphospholipid syndrome on fatty acid oxidation in trophoblast cells.
Arch Gynecol Obstet. 2015 Sep; 292(3):559-67.AG

Abstract

BACKGROUND AND AIMS

The role of metabolic disorders of long-chain fatty acid oxidation in the development of pre-eclampsia (PE), hemolysis, elevated liver enzymes, and low platelets (HELLP) syndrome and antiphospholipid syndrome (APS) is unclear. The aim of this study was to research the effects of three serum on fatty acid oxidation in trophoblast cells.

METHODS

Primary human trophoblast cells and HTR8/SVneo cells were treated with serum from patients with early-onset severe PE (E-PE), E-PE with HELLP (PE-HELLP), APS, and normal pregnant women as controls (NC). Cells treated with free fatty acids (FFAs) of various lengths were used as controls.

RESULTS

Triglyceride (TG) and FFA levels of the E-PE and APS groups were significantly higher than the PE-HELLP and NC groups (P < 0.05). Trophoblast cells treated with serum from the E-PE and APS groups showed obvious morphological changes and a large amount of lipid droplet deposition. Cells in the E-PE group had more severe damage of mitochondria ultrastructure, presenting with cell morphological changes, lipid droplet deposition, and mitochondria damage similar to the long-chain FFA group. FFA levels in the PE-HELLP group increased significantly compared with the NC group (P < 0.05), while TG levels did not change significantly (P > 0.05). Trophoblast cells treated with serum from the PE-HELLP group showed cellular morphology and mitochondria changes similar to the E-PE group, but had relatively less lipid droplet deposition.

CONCLUSION

Serum from patients with E-PE, PE-HELLP, and APS with elevated levels of FFA had different effects on trophoblast cells, including cell morphology, lipid droplets deposition, and mitochondrial ultrastructure, suggesting disorders of lipid metabolism and fatty acid oxidation of various degrees and types. Serum from patients with E-PE led to damage similar to that of long-chain FFA in cell morphology, lipid droplet deposition and mitochondrial ultrastructure, indicating the correlation between disorders of long-chain fatty acid oxidation and the development of the disease.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, 100191, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25740297

Citation

Yu, Huan, et al. "Effects of Serum From Patients With Early-onset Pre-eclampsia, HELLP Syndrome, and Antiphospholipid Syndrome On Fatty Acid Oxidation in Trophoblast Cells." Archives of Gynecology and Obstetrics, vol. 292, no. 3, 2015, pp. 559-67.
Yu H, Yang Z, Ding X, et al. Effects of serum from patients with early-onset pre-eclampsia, HELLP syndrome, and antiphospholipid syndrome on fatty acid oxidation in trophoblast cells. Arch Gynecol Obstet. 2015;292(3):559-67.
Yu, H., Yang, Z., Ding, X., Wang, Y., & Han, Y. (2015). Effects of serum from patients with early-onset pre-eclampsia, HELLP syndrome, and antiphospholipid syndrome on fatty acid oxidation in trophoblast cells. Archives of Gynecology and Obstetrics, 292(3), 559-67. https://doi.org/10.1007/s00404-015-3669-2
Yu H, et al. Effects of Serum From Patients With Early-onset Pre-eclampsia, HELLP Syndrome, and Antiphospholipid Syndrome On Fatty Acid Oxidation in Trophoblast Cells. Arch Gynecol Obstet. 2015;292(3):559-67. PubMed PMID: 25740297.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of serum from patients with early-onset pre-eclampsia, HELLP syndrome, and antiphospholipid syndrome on fatty acid oxidation in trophoblast cells. AU - Yu,Huan, AU - Yang,Zi, AU - Ding,Xiaoyan, AU - Wang,Yanling, AU - Han,Yiwei, Y1 - 2015/03/05/ PY - 2014/08/15/received PY - 2015/02/16/accepted PY - 2015/3/6/entrez PY - 2015/3/6/pubmed PY - 2016/1/30/medline SP - 559 EP - 67 JF - Archives of gynecology and obstetrics JO - Arch Gynecol Obstet VL - 292 IS - 3 N2 - BACKGROUND AND AIMS: The role of metabolic disorders of long-chain fatty acid oxidation in the development of pre-eclampsia (PE), hemolysis, elevated liver enzymes, and low platelets (HELLP) syndrome and antiphospholipid syndrome (APS) is unclear. The aim of this study was to research the effects of three serum on fatty acid oxidation in trophoblast cells. METHODS: Primary human trophoblast cells and HTR8/SVneo cells were treated with serum from patients with early-onset severe PE (E-PE), E-PE with HELLP (PE-HELLP), APS, and normal pregnant women as controls (NC). Cells treated with free fatty acids (FFAs) of various lengths were used as controls. RESULTS: Triglyceride (TG) and FFA levels of the E-PE and APS groups were significantly higher than the PE-HELLP and NC groups (P < 0.05). Trophoblast cells treated with serum from the E-PE and APS groups showed obvious morphological changes and a large amount of lipid droplet deposition. Cells in the E-PE group had more severe damage of mitochondria ultrastructure, presenting with cell morphological changes, lipid droplet deposition, and mitochondria damage similar to the long-chain FFA group. FFA levels in the PE-HELLP group increased significantly compared with the NC group (P < 0.05), while TG levels did not change significantly (P > 0.05). Trophoblast cells treated with serum from the PE-HELLP group showed cellular morphology and mitochondria changes similar to the E-PE group, but had relatively less lipid droplet deposition. CONCLUSION: Serum from patients with E-PE, PE-HELLP, and APS with elevated levels of FFA had different effects on trophoblast cells, including cell morphology, lipid droplets deposition, and mitochondrial ultrastructure, suggesting disorders of lipid metabolism and fatty acid oxidation of various degrees and types. Serum from patients with E-PE led to damage similar to that of long-chain FFA in cell morphology, lipid droplet deposition and mitochondrial ultrastructure, indicating the correlation between disorders of long-chain fatty acid oxidation and the development of the disease. SN - 1432-0711 UR - https://www.unboundmedicine.com/medline/citation/25740297/Effects_of_serum_from_patients_with_early_onset_pre_eclampsia_HELLP_syndrome_and_antiphospholipid_syndrome_on_fatty_acid_oxidation_in_trophoblast_cells_ L2 - https://dx.doi.org/10.1007/s00404-015-3669-2 DB - PRIME DP - Unbound Medicine ER -