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High-level association of bovine digital dermatitis Treponema spp. with contagious ovine digital dermatitis lesions and presence of Fusobacterium necrophorum and Dichelobacter nodosus.
J Clin Microbiol. 2015 May; 53(5):1628-38.JC

Abstract

Contagious ovine digital dermatitis (CODD) is an important foot disease in sheep, with significant animal welfare and economic implications. It is thought that CODD emerged from bovine digital dermatitis (BDD) via treponemal bacteria. With wildlife species such as elk now suffering a CODD-like disease, it is imperative to clarify these disease etiologies. A large investigation into treponemal association with CODD is warranted. CODD lesions (n = 58) and healthy sheep foot tissues (n = 56) were analyzed by PCR for the three BDD-associated Treponema phylogroups and two other lameness-associated bacteria, Dichelobacter nodosus and Fusobacterium necrophorum. Spirochete culture was also attempted on CODD lesions. "Treponema medium/Treponema vincentii-like," "Treponema phagedenis-like," and Treponema pedis spirochetes were identified in 39/58 (67%), 49/58 (85%), and 41/58 (71%) of CODD lesions, respectively. One or more BDD-associated Treponema phylogroups were detected in 100% of CODD lesions. Healthy foot tissues did not amplify BDD-associated Treponema phylogroup DNA. D. nodosus and F. necrophorum were present in 34/58 (59%) and 41/58 (71%) of CODD lesions and 22/56 (39%) and 5/56 (9%) of healthy foot tissues, respectively. Thirty-two spirochetes were isolated from CODD lesions, with representatives clustering with, and indistinguishable from, each of the three BDD-associated Treponema phylogroups based on 16S rRNA gene comparisons. This study for the first time demonstrates a high-level association for BDD treponeme phylogroups in CODD and their absence from healthy tissues, supporting the hypothesis that BDD treponemes play a primary causative role in CODD and confirming that the specific PCR assays are an effective differential diagnostic tool for CODD.

Authors+Show Affiliations

Department of Infection Biology, School of Veterinary Science, Institute of Infection and Global Health, Liverpool, Merseyside, United Kingdom l.sullivan@liverpool.ac.uk.Department of Infection Biology, School of Veterinary Science, Institute of Infection and Global Health, Liverpool, Merseyside, United Kingdom.Department of Epidemiology and Population Health, Institute of Infection and Global Health, University of Liverpool, Leahurst Campus, Neston, Wirral, Cheshire, United Kingdom.Department of Infection Biology, School of Veterinary Science, Institute of Infection and Global Health, Liverpool, Merseyside, United Kingdom.University of Liverpool & Wood Veterinary Group, Gloucester, Gloucestershire, United Kingdom.Department of Infection Biology, School of Veterinary Science, Institute of Infection and Global Health, Liverpool, Merseyside, United Kingdom.Department of Infection Biology, School of Veterinary Science, Institute of Infection and Global Health, Liverpool, Merseyside, United Kingdom.Department of Epidemiology and Population Health, Institute of Infection and Global Health, University of Liverpool, Leahurst Campus, Neston, Wirral, Cheshire, United Kingdom.Department of Epidemiology and Population Health, Institute of Infection and Global Health, University of Liverpool, Leahurst Campus, Neston, Wirral, Cheshire, United Kingdom.Department of Infection Biology, School of Veterinary Science, Institute of Infection and Global Health, Liverpool, Merseyside, United Kingdom.Department of Infection Biology, School of Veterinary Science, Institute of Infection and Global Health, Liverpool, Merseyside, United Kingdom.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25740778

Citation

Sullivan, L E., et al. "High-level Association of Bovine Digital Dermatitis Treponema Spp. With Contagious Ovine Digital Dermatitis Lesions and Presence of Fusobacterium Necrophorum and Dichelobacter Nodosus." Journal of Clinical Microbiology, vol. 53, no. 5, 2015, pp. 1628-38.
Sullivan LE, Clegg SR, Angell JW, et al. High-level association of bovine digital dermatitis Treponema spp. with contagious ovine digital dermatitis lesions and presence of Fusobacterium necrophorum and Dichelobacter nodosus. J Clin Microbiol. 2015;53(5):1628-38.
Sullivan, L. E., Clegg, S. R., Angell, J. W., Newbrook, K., Blowey, R. W., Carter, S. D., Bell, J., Duncan, J. S., Grove-White, D. H., Murray, R. D., & Evans, N. J. (2015). High-level association of bovine digital dermatitis Treponema spp. with contagious ovine digital dermatitis lesions and presence of Fusobacterium necrophorum and Dichelobacter nodosus. Journal of Clinical Microbiology, 53(5), 1628-38. https://doi.org/10.1128/JCM.00180-15
Sullivan LE, et al. High-level Association of Bovine Digital Dermatitis Treponema Spp. With Contagious Ovine Digital Dermatitis Lesions and Presence of Fusobacterium Necrophorum and Dichelobacter Nodosus. J Clin Microbiol. 2015;53(5):1628-38. PubMed PMID: 25740778.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High-level association of bovine digital dermatitis Treponema spp. with contagious ovine digital dermatitis lesions and presence of Fusobacterium necrophorum and Dichelobacter nodosus. AU - Sullivan,L E, AU - Clegg,S R, AU - Angell,J W, AU - Newbrook,K, AU - Blowey,R W, AU - Carter,S D, AU - Bell,J, AU - Duncan,J S, AU - Grove-White,D H, AU - Murray,R D, AU - Evans,N J, Y1 - 2015/03/04/ PY - 2015/01/26/received PY - 2015/02/27/accepted PY - 2015/3/6/entrez PY - 2015/3/6/pubmed PY - 2015/12/30/medline SP - 1628 EP - 38 JF - Journal of clinical microbiology JO - J. Clin. Microbiol. VL - 53 IS - 5 N2 - Contagious ovine digital dermatitis (CODD) is an important foot disease in sheep, with significant animal welfare and economic implications. It is thought that CODD emerged from bovine digital dermatitis (BDD) via treponemal bacteria. With wildlife species such as elk now suffering a CODD-like disease, it is imperative to clarify these disease etiologies. A large investigation into treponemal association with CODD is warranted. CODD lesions (n = 58) and healthy sheep foot tissues (n = 56) were analyzed by PCR for the three BDD-associated Treponema phylogroups and two other lameness-associated bacteria, Dichelobacter nodosus and Fusobacterium necrophorum. Spirochete culture was also attempted on CODD lesions. "Treponema medium/Treponema vincentii-like," "Treponema phagedenis-like," and Treponema pedis spirochetes were identified in 39/58 (67%), 49/58 (85%), and 41/58 (71%) of CODD lesions, respectively. One or more BDD-associated Treponema phylogroups were detected in 100% of CODD lesions. Healthy foot tissues did not amplify BDD-associated Treponema phylogroup DNA. D. nodosus and F. necrophorum were present in 34/58 (59%) and 41/58 (71%) of CODD lesions and 22/56 (39%) and 5/56 (9%) of healthy foot tissues, respectively. Thirty-two spirochetes were isolated from CODD lesions, with representatives clustering with, and indistinguishable from, each of the three BDD-associated Treponema phylogroups based on 16S rRNA gene comparisons. This study for the first time demonstrates a high-level association for BDD treponeme phylogroups in CODD and their absence from healthy tissues, supporting the hypothesis that BDD treponemes play a primary causative role in CODD and confirming that the specific PCR assays are an effective differential diagnostic tool for CODD. SN - 1098-660X UR - https://www.unboundmedicine.com/medline/citation/25740778/High_level_association_of_bovine_digital_dermatitis_Treponema_spp__with_contagious_ovine_digital_dermatitis_lesions_and_presence_of_Fusobacterium_necrophorum_and_Dichelobacter_nodosus_ L2 - http://jcm.asm.org/cgi/pmidlookup?view=long&pmid=25740778 DB - PRIME DP - Unbound Medicine ER -