Tags

Type your tag names separated by a space and hit enter

The long noncoding RNA SPRY4-IT1 increases the proliferation of human breast cancer cells by upregulating ZNF703 expression.
Mol Cancer 2015; 14:51MC

Abstract

BACKGROUND

Long noncoding RNAs (lncRNAs) have emerged recently as a new class of genes that regulate cellular processes, such as cell growth and apoptosis. The SPRY4 intronic transcript 1 (SPRY4-IT1) is a 708-bp lncRNA on chromosome 5 with a potential functional role in tumorigenesis. The clinical significance of SPRY4-IT1 and the effect of SPRY4-IT1 on cancer progression are unclear.

METHODS

Quantitative reverse transcriptase PCR (qRT-PCR) was performed to investigate the expression of SPRY4-IT1 in 48 breast cancer tissues and four breast cancer cell lines. Gain and loss of function approaches were used to investigate the biological role of SPRY4-IT1 in vitro. Microarray bioinformatics analysis was performed to identify the putative targets of SPRY4-IT1, which were further verified by rescue experiments, and by western blotting and qRT-PCR.

RESULTS

SPRY4-IT1 expression was significantly upregulated in 48 breast cancer tumor tissues comparedwith normal tissues. Additionally, increased SPRY4-IT1 expression was found to be associated with a larger tumor size and an advanced pathological stage in breast cancer patients. The knockdown of SPRY4-IT1 significantly suppressed proliferation and caused apoptosis of breast cancer cells in vitro. Furthermore, we discovered that ZNF703 was a target of SPRY4-IT1 and was downregulated by SPRY4-IT1 knockdown. Moreover, we provide the first demonstration that ZNF703 plays an oncogenic role in ER (-) breast carcinoma cells.

CONCLUSIONS

SPRY4-IT1 is a novel prognostic biomarker and a potential therapeutic candidate for breast cancer.

Authors+Show Affiliations

Department of Oncology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, PR China. 1138303166@qq.com. Taixing People's Hospital, Taixing, Jiangsu, PR China. 1138303166@qq.com.Department of Oncology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, PR China. lijuan123@163.com.Taixing People's Hospital, Taixing, Jiangsu, PR China. liuyctx@163.com.Department of Oncology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, PR China. 430397952@qq.com.Department of Oncology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, PR China. yingrui668@163.com.Department of Oncology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, PR China. 82560036@qq.com.Department of Oncology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, PR China. 911165@qq.com.Department of Oncology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, PR China. Yifan23@sohu.com.Department of Oncology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, PR China. kemingwang@njmu.edu.cn.Department of Oncology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, PR China. shuyongqian@csco.org.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25742952

Citation

Shi, Yongguo, et al. "The Long Noncoding RNA SPRY4-IT1 Increases the Proliferation of Human Breast Cancer Cells By Upregulating ZNF703 Expression." Molecular Cancer, vol. 14, 2015, p. 51.
Shi Y, Li J, Liu Y, et al. The long noncoding RNA SPRY4-IT1 increases the proliferation of human breast cancer cells by upregulating ZNF703 expression. Mol Cancer. 2015;14:51.
Shi, Y., Li, J., Liu, Y., Ding, J., Fan, Y., Tian, Y., ... Shu, Y. (2015). The long noncoding RNA SPRY4-IT1 increases the proliferation of human breast cancer cells by upregulating ZNF703 expression. Molecular Cancer, 14, p. 51. doi:10.1186/s12943-015-0318-0.
Shi Y, et al. The Long Noncoding RNA SPRY4-IT1 Increases the Proliferation of Human Breast Cancer Cells By Upregulating ZNF703 Expression. Mol Cancer. 2015 Feb 22;14:51. PubMed PMID: 25742952.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The long noncoding RNA SPRY4-IT1 increases the proliferation of human breast cancer cells by upregulating ZNF703 expression. AU - Shi,Yongguo, AU - Li,Juan, AU - Liu,Yangchen, AU - Ding,Jie, AU - Fan,Yingrui, AU - Tian,Yun, AU - Wang,Li, AU - Lian,Yifan, AU - Wang,Keming, AU - Shu,Yongqian, Y1 - 2015/02/22/ PY - 2014/11/02/received PY - 2015/02/09/accepted PY - 2015/3/7/entrez PY - 2015/3/10/pubmed PY - 2016/1/12/medline SP - 51 EP - 51 JF - Molecular cancer JO - Mol. Cancer VL - 14 N2 - BACKGROUND: Long noncoding RNAs (lncRNAs) have emerged recently as a new class of genes that regulate cellular processes, such as cell growth and apoptosis. The SPRY4 intronic transcript 1 (SPRY4-IT1) is a 708-bp lncRNA on chromosome 5 with a potential functional role in tumorigenesis. The clinical significance of SPRY4-IT1 and the effect of SPRY4-IT1 on cancer progression are unclear. METHODS: Quantitative reverse transcriptase PCR (qRT-PCR) was performed to investigate the expression of SPRY4-IT1 in 48 breast cancer tissues and four breast cancer cell lines. Gain and loss of function approaches were used to investigate the biological role of SPRY4-IT1 in vitro. Microarray bioinformatics analysis was performed to identify the putative targets of SPRY4-IT1, which were further verified by rescue experiments, and by western blotting and qRT-PCR. RESULTS: SPRY4-IT1 expression was significantly upregulated in 48 breast cancer tumor tissues comparedwith normal tissues. Additionally, increased SPRY4-IT1 expression was found to be associated with a larger tumor size and an advanced pathological stage in breast cancer patients. The knockdown of SPRY4-IT1 significantly suppressed proliferation and caused apoptosis of breast cancer cells in vitro. Furthermore, we discovered that ZNF703 was a target of SPRY4-IT1 and was downregulated by SPRY4-IT1 knockdown. Moreover, we provide the first demonstration that ZNF703 plays an oncogenic role in ER (-) breast carcinoma cells. CONCLUSIONS: SPRY4-IT1 is a novel prognostic biomarker and a potential therapeutic candidate for breast cancer. SN - 1476-4598 UR - https://www.unboundmedicine.com/medline/citation/25742952/The_long_noncoding_RNA_SPRY4_IT1_increases_the_proliferation_of_human_breast_cancer_cells_by_upregulating_ZNF703_expression_ L2 - https://molecular-cancer.biomedcentral.com/articles/10.1186/s12943-015-0318-0 DB - PRIME DP - Unbound Medicine ER -