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Antinociceptive activity of transient receptor potential channel TRPV1, TRPA1, and TRPM8 antagonists in neurogenic and neuropathic pain models in mice.
J Zhejiang Univ Sci B. 2015 Mar; 16(3):167-78.JZ

Abstract

The aim of this research was to assess the antinociceptive activity of the transient receptor potential (TRP) channel TRPV1, TRPM8, and TRPA1 antagonists in neurogenic, tonic, and neuropathic pain models in mice. For this purpose, TRP channel antagonists were administered into the dorsal surface of a hind paw 15 min before capsaicin, allyl isothiocyanate (AITC), or formalin. Their antiallodynic and antihyperalgesic efficacies after intraperitoneal administration were also assessed in a paclitaxel-induced neuropathic pain model. Motor coordination of paclitaxel-treated mice that received these TRP channel antagonists was investigated using the rotarod test. TRPV1 antagonists, capsazepine and SB-366791, attenuated capsaicin-induced nociceptive reaction in a concentration-dependent manner. At 8 µg/20 µl, this effect was 51% (P<0.001) for capsazepine and 37% (P<0.05) for SB-366791. A TRPA1 antagonist, A-967079, reduced pain reaction by 48% (P<0.05) in the AITC test and by 54% (P<0.001) in the early phase of the formalin test. The test compounds had no influence on the late phase of the formalin test. In paclitaxel-treated mice, they did not attenuate heat hyperalgesia but N-(3-aminopropyl)-2-{[(3-methylphenyl)methyl]oxy}-N-(2-thienylmethyl) benzamide hydrochloride salt (AMTB), a TRPM8 antagonist, reduced cold hyperalgesia and tactile allodynia by 31% (P<0.05) and 51% (P<0.01), respectively. HC-030031, a TRPA1 channel antagonist, attenuated tactile allodynia in the von Frey test (62%; P<0.001). In conclusion, distinct members of TRP channel family are involved in different pain models in mice. Antagonists of TRP channels attenuate nocifensive responses of neurogenic, tonic, and neuropathic pain, but their efficacies strongly depend on the pain model used.

Authors+Show Affiliations

Department of Pharmacodynamics, Faculty of Pharmacy, Jagiellonian University, Medyczna 9, 30-688 Cracow, Poland.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25743118

Citation

Sałat, Kinga, and Barbara Filipek. "Antinociceptive Activity of Transient Receptor Potential Channel TRPV1, TRPA1, and TRPM8 Antagonists in Neurogenic and Neuropathic Pain Models in Mice." Journal of Zhejiang University. Science. B, vol. 16, no. 3, 2015, pp. 167-78.
Sałat K, Filipek B. Antinociceptive activity of transient receptor potential channel TRPV1, TRPA1, and TRPM8 antagonists in neurogenic and neuropathic pain models in mice. J Zhejiang Univ Sci B. 2015;16(3):167-78.
Sałat, K., & Filipek, B. (2015). Antinociceptive activity of transient receptor potential channel TRPV1, TRPA1, and TRPM8 antagonists in neurogenic and neuropathic pain models in mice. Journal of Zhejiang University. Science. B, 16(3), 167-78. https://doi.org/10.1631/jzus.B1400189
Sałat K, Filipek B. Antinociceptive Activity of Transient Receptor Potential Channel TRPV1, TRPA1, and TRPM8 Antagonists in Neurogenic and Neuropathic Pain Models in Mice. J Zhejiang Univ Sci B. 2015;16(3):167-78. PubMed PMID: 25743118.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antinociceptive activity of transient receptor potential channel TRPV1, TRPA1, and TRPM8 antagonists in neurogenic and neuropathic pain models in mice. AU - Sałat,Kinga, AU - Filipek,Barbara, PY - 2015/3/7/entrez PY - 2015/3/7/pubmed PY - 2015/12/15/medline KW - Allyl isothiocyanate KW - Capsaicin KW - Formalin KW - Neurogenic pain KW - Paclitaxel-induced sensory neuropathy KW - Transient receptor potential channels SP - 167 EP - 78 JF - Journal of Zhejiang University. Science. B JO - J Zhejiang Univ Sci B VL - 16 IS - 3 N2 - The aim of this research was to assess the antinociceptive activity of the transient receptor potential (TRP) channel TRPV1, TRPM8, and TRPA1 antagonists in neurogenic, tonic, and neuropathic pain models in mice. For this purpose, TRP channel antagonists were administered into the dorsal surface of a hind paw 15 min before capsaicin, allyl isothiocyanate (AITC), or formalin. Their antiallodynic and antihyperalgesic efficacies after intraperitoneal administration were also assessed in a paclitaxel-induced neuropathic pain model. Motor coordination of paclitaxel-treated mice that received these TRP channel antagonists was investigated using the rotarod test. TRPV1 antagonists, capsazepine and SB-366791, attenuated capsaicin-induced nociceptive reaction in a concentration-dependent manner. At 8 µg/20 µl, this effect was 51% (P<0.001) for capsazepine and 37% (P<0.05) for SB-366791. A TRPA1 antagonist, A-967079, reduced pain reaction by 48% (P<0.05) in the AITC test and by 54% (P<0.001) in the early phase of the formalin test. The test compounds had no influence on the late phase of the formalin test. In paclitaxel-treated mice, they did not attenuate heat hyperalgesia but N-(3-aminopropyl)-2-{[(3-methylphenyl)methyl]oxy}-N-(2-thienylmethyl) benzamide hydrochloride salt (AMTB), a TRPM8 antagonist, reduced cold hyperalgesia and tactile allodynia by 31% (P<0.05) and 51% (P<0.01), respectively. HC-030031, a TRPA1 channel antagonist, attenuated tactile allodynia in the von Frey test (62%; P<0.001). In conclusion, distinct members of TRP channel family are involved in different pain models in mice. Antagonists of TRP channels attenuate nocifensive responses of neurogenic, tonic, and neuropathic pain, but their efficacies strongly depend on the pain model used. SN - 1862-1783 UR - https://www.unboundmedicine.com/medline/citation/25743118/Antinociceptive_activity_of_transient_receptor_potential_channel_TRPV1_TRPA1_and_TRPM8_antagonists_in_neurogenic_and_neuropathic_pain_models_in_mice_ L2 - http://www.jzus.zju.edu.cn/article.php?doi=10.1631/jzus.B1400189 DB - PRIME DP - Unbound Medicine ER -