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Occurrence of antibodies against Toxoplasma gondii and its isolation and genotyping in donkeys, mules, and horses in Brazil.
Vet Parasitol. 2015 Apr 15; 209(1-2):129-32.VP

Abstract

The occurrence of antibodies against Toxoplasma gondii was determined in donkeys, mules, and horses from different regions of Brazil. Serum samples from 304 donkeys (67.11%), 118 horses (26.05%), and 31 mules (6.84%) were analyzed by means of the indirect fluorescent antibody test (cutoff=64). Antibodies against T. gondii were detected in 129 equids (28.47%) (82 donkeys, 32 horses, and 15 mules). Tissue samples from 19 seropositive and 50 seronegative animals were obtained in order to isolate the parasite by means of mouse bioassay, and T. gondii was isolated from a donkey. Through genotypic characterization of the isolate, by means of polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) using 11 genotypic markers, the genotype #163 (TgCkBr220), which has already been described in chickens in Brazil, was identified.

Authors+Show Affiliations

Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Orlando Marques de Paiva, 87, CEP 05508-270 São Paulo, SP, Brazil. Electronic address: sgennari@usp.br.Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Orlando Marques de Paiva, 87, CEP 05508-270 São Paulo, SP, Brazil.Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Orlando Marques de Paiva, 87, CEP 05508-270 São Paulo, SP, Brazil.Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Orlando Marques de Paiva, 87, CEP 05508-270 São Paulo, SP, Brazil.Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Orlando Marques de Paiva, 87, CEP 05508-270 São Paulo, SP, Brazil; Curso de Medicina Veterinária, FMU, São Paulo, SP, Brazil.Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Orlando Marques de Paiva, 87, CEP 05508-270 São Paulo, SP, Brazil.Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Orlando Marques de Paiva, 87, CEP 05508-270 São Paulo, SP, Brazil.Colegiado de Medicina Veterinária, Universidade Federal do Vale do São Francisco, Petrolina, PE, Brazil.Instituto de Teologia Aplicada, Faculdades INTA, Sobral, CE, Brazil.Instituto de Ciências Biológicas, UFMG, Belo Horizonte, MG, Brazil.Instituto Biológico, São Paulo, SP, Brazil.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25747488

Citation

Gennari, Solange M., et al. "Occurrence of Antibodies Against Toxoplasma Gondii and Its Isolation and Genotyping in Donkeys, Mules, and Horses in Brazil." Veterinary Parasitology, vol. 209, no. 1-2, 2015, pp. 129-32.
Gennari SM, Esmerini Pde O, Lopes MG, et al. Occurrence of antibodies against Toxoplasma gondii and its isolation and genotyping in donkeys, mules, and horses in Brazil. Vet Parasitol. 2015;209(1-2):129-32.
Gennari, S. M., Esmerini, P. d. e. . O., Lopes, M. G., Soares, H. S., Vitaliano, S. N., Cabral, A. D., Pena, H. F., Horta, M. C., Cavalcante, P. H., Fortes, K. P., & Villalobos, E. M. (2015). Occurrence of antibodies against Toxoplasma gondii and its isolation and genotyping in donkeys, mules, and horses in Brazil. Veterinary Parasitology, 209(1-2), 129-32. https://doi.org/10.1016/j.vetpar.2015.01.023
Gennari SM, et al. Occurrence of Antibodies Against Toxoplasma Gondii and Its Isolation and Genotyping in Donkeys, Mules, and Horses in Brazil. Vet Parasitol. 2015 Apr 15;209(1-2):129-32. PubMed PMID: 25747488.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Occurrence of antibodies against Toxoplasma gondii and its isolation and genotyping in donkeys, mules, and horses in Brazil. AU - Gennari,Solange M, AU - Esmerini,Patrícia de O, AU - Lopes,Marcos G, AU - Soares,Herbert S, AU - Vitaliano,Sérgio N, AU - Cabral,Aline D, AU - Pena,Hilda F J, AU - Horta,Maurício C, AU - Cavalcante,Paulo H, AU - Fortes,Kleber P, AU - Villalobos,Eliana M C, Y1 - 2015/02/04/ PY - 2014/07/16/received PY - 2015/01/22/revised PY - 2015/01/25/accepted PY - 2015/3/10/entrez PY - 2015/3/10/pubmed PY - 2016/3/24/medline KW - Brazil KW - Equidae KW - Genotyping KW - Isolation KW - Toxoplasma gondii SP - 129 EP - 32 JF - Veterinary parasitology JO - Vet Parasitol VL - 209 IS - 1-2 N2 - The occurrence of antibodies against Toxoplasma gondii was determined in donkeys, mules, and horses from different regions of Brazil. Serum samples from 304 donkeys (67.11%), 118 horses (26.05%), and 31 mules (6.84%) were analyzed by means of the indirect fluorescent antibody test (cutoff=64). Antibodies against T. gondii were detected in 129 equids (28.47%) (82 donkeys, 32 horses, and 15 mules). Tissue samples from 19 seropositive and 50 seronegative animals were obtained in order to isolate the parasite by means of mouse bioassay, and T. gondii was isolated from a donkey. Through genotypic characterization of the isolate, by means of polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) using 11 genotypic markers, the genotype #163 (TgCkBr220), which has already been described in chickens in Brazil, was identified. SN - 1873-2550 UR - https://www.unboundmedicine.com/medline/citation/25747488/Occurrence_of_antibodies_against_Toxoplasma_gondii_and_its_isolation_and_genotyping_in_donkeys_mules_and_horses_in_Brazil_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-4017(15)00041-2 DB - PRIME DP - Unbound Medicine ER -