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Antimicrobial resistance, virulence factors and genetic lineages of hospital-onset methicillin-resistant Staphylococcus aureus isolates detected in a hospital in Zaragoza.
Enferm Infecc Microbiol Clin. 2015 Nov; 33(9):590-6.EI

Abstract

INTRODUCTION

MRSA population dynamics is undergoing significant changes, and for this reason it is important to know which clones are circulating in our nosocomial environment.

MATERIALS AND METHODS

A total of 118 MRSA isolates were collected from clinical samples from patients with previous hospital or healthcare contact (named as hospital-onset MRSA (HO-MRSA)) during a one year period. Susceptibility testing was performed by disk diffusion and microdilution. The presence of resistance genes and virulence factors were tested by PCR. All isolates were typed by SCCmec, spa and agr typing. PFGE and MLST were applied to a selection of them.

RESULTS

Eighty-three HO-MRSA isolates (70.3%) were resistant to any antibiotic included in the macrolide-lincosamide-streptogramin B group. Among these isolates, the M phenotype was the most frequent (73.5%). One hundred and seven of HO-MRSA isolates (90.7%) showed aminoglycoside resistance. The combination aac(6')-Ie-aph(2″)-Ia+ant(4')-Ia genes was the most frequent (22.4%). Tetracycline resistance rates in HO-MRSA isolates were low (3.4%), although a high level of mupirocin resistance was observed (25.4%). Most of the HO-MRSA isolates (approximately 90%) showed SCCmec type IVc and agr type II. Fifteen unrelated pulsotypes were identified. CC5 was the most prevalent (88.1%), followed by CC8 (5.9%), CC22 (2.5%), CC398 (2.5%) and CC1 (0.8%).

CONCLUSION

CC5/ST125/t067 lineage was the most frequent. This lineage was related to aminoglycoside resistance, and to a lesser extent, with macrolide resistance. The presence of international clones as EMRSA-15 (CC22/ST22), European clones as CC5/ST228, community clones related to CC1 or CC8 and livestock associated clones, as CC398, were observed in a low percentage.

Authors+Show Affiliations

Servicio de Microbiología, Hospital Clínico Universitario Lozano Blesa, San Juan Bosco s/n, Zaragoza, Spain.Servicio de Microbiología, Hospital Clínico Universitario Lozano Blesa y Departamento de Microbiología, Facultad de Medicina, Universidad de Zaragoza, San Juan Bosco s/n, Zaragoza, Spain. Electronic address: cseral@unizar.es.Servicio de Microbiología y Unidad de Apoyo a la Investigación, Instituto de Investigación Biomédica de Vigo (IBIV), Complexo Hospitalario Universitario de Vigo (CHUVI), C/ Pizarro, 22, Vigo, Pontevedra, Spain.Unidad de Apoyo a la Investigación, Instituto de Investigación Biomédica de Vigo (IBIV), Complejo Hospitalario Universitario de Vigo (CHUVI), C/ Pizarro, 22, Vigo, Pontevedra, Spain.Servicio de Microbiología, Hospital Clínico Universitario Lozano Blesa, San Juan Bosco s/n, Zaragoza, Spain.Servicio de Microbiología, Hospital Clínico Universitario Lozano Blesa, San Juan Bosco s/n, Zaragoza, Spain.Servicio de Microbiología y Unidad de Apoyo a la Investigación, Instituto de Investigación Biomédica de Vigo (IBIV), Complexo Hospitalario Universitario de Vigo (CHUVI), C/ Pizarro, 22, Vigo, Pontevedra, Spain.Servicio de Microbiología, Hospital Clínico Universitario Lozano Blesa y Departamento de Microbiología, Facultad de Medicina, Universidad de Zaragoza, San Juan Bosco s/n, Zaragoza, Spain.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25749415

Citation

González-Domínguez, María, et al. "Antimicrobial Resistance, Virulence Factors and Genetic Lineages of Hospital-onset Methicillin-resistant Staphylococcus Aureus Isolates Detected in a Hospital in Zaragoza." Enfermedades Infecciosas Y Microbiologia Clinica, vol. 33, no. 9, 2015, pp. 590-6.
González-Domínguez M, Seral C, Potel C, et al. Antimicrobial resistance, virulence factors and genetic lineages of hospital-onset methicillin-resistant Staphylococcus aureus isolates detected in a hospital in Zaragoza. Enferm Infecc Microbiol Clin. 2015;33(9):590-6.
González-Domínguez, M., Seral, C., Potel, C., Constenla, L., Algarate, S., Gude, M. J., Álvarez, M., & Castillo, F. J. (2015). Antimicrobial resistance, virulence factors and genetic lineages of hospital-onset methicillin-resistant Staphylococcus aureus isolates detected in a hospital in Zaragoza. Enfermedades Infecciosas Y Microbiologia Clinica, 33(9), 590-6. https://doi.org/10.1016/j.eimc.2015.01.015
González-Domínguez M, et al. Antimicrobial Resistance, Virulence Factors and Genetic Lineages of Hospital-onset Methicillin-resistant Staphylococcus Aureus Isolates Detected in a Hospital in Zaragoza. Enferm Infecc Microbiol Clin. 2015;33(9):590-6. PubMed PMID: 25749415.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antimicrobial resistance, virulence factors and genetic lineages of hospital-onset methicillin-resistant Staphylococcus aureus isolates detected in a hospital in Zaragoza. AU - González-Domínguez,María, AU - Seral,Cristina, AU - Potel,Carmen, AU - Constenla,Lucía, AU - Algarate,Sonia, AU - Gude,M José, AU - Álvarez,Maximiliano, AU - Castillo,Francisco Javier, Y1 - 2015/03/04/ PY - 2014/11/06/received PY - 2015/01/16/revised PY - 2015/01/27/accepted PY - 2015/3/10/entrez PY - 2015/3/10/pubmed PY - 2017/6/22/medline KW - Clonal lineages KW - Genes de resistencia KW - Hospital-onset KW - Líneas clonales KW - MRSA KW - Molecular typing KW - Origen hospitalario KW - Resistance genes KW - SARM KW - Tipado molecular SP - 590 EP - 6 JF - Enfermedades infecciosas y microbiologia clinica JO - Enferm Infecc Microbiol Clin VL - 33 IS - 9 N2 - INTRODUCTION: MRSA population dynamics is undergoing significant changes, and for this reason it is important to know which clones are circulating in our nosocomial environment. MATERIALS AND METHODS: A total of 118 MRSA isolates were collected from clinical samples from patients with previous hospital or healthcare contact (named as hospital-onset MRSA (HO-MRSA)) during a one year period. Susceptibility testing was performed by disk diffusion and microdilution. The presence of resistance genes and virulence factors were tested by PCR. All isolates were typed by SCCmec, spa and agr typing. PFGE and MLST were applied to a selection of them. RESULTS: Eighty-three HO-MRSA isolates (70.3%) were resistant to any antibiotic included in the macrolide-lincosamide-streptogramin B group. Among these isolates, the M phenotype was the most frequent (73.5%). One hundred and seven of HO-MRSA isolates (90.7%) showed aminoglycoside resistance. The combination aac(6')-Ie-aph(2″)-Ia+ant(4')-Ia genes was the most frequent (22.4%). Tetracycline resistance rates in HO-MRSA isolates were low (3.4%), although a high level of mupirocin resistance was observed (25.4%). Most of the HO-MRSA isolates (approximately 90%) showed SCCmec type IVc and agr type II. Fifteen unrelated pulsotypes were identified. CC5 was the most prevalent (88.1%), followed by CC8 (5.9%), CC22 (2.5%), CC398 (2.5%) and CC1 (0.8%). CONCLUSION: CC5/ST125/t067 lineage was the most frequent. This lineage was related to aminoglycoside resistance, and to a lesser extent, with macrolide resistance. The presence of international clones as EMRSA-15 (CC22/ST22), European clones as CC5/ST228, community clones related to CC1 or CC8 and livestock associated clones, as CC398, were observed in a low percentage. SN - 1578-1852 UR - https://www.unboundmedicine.com/medline/citation/25749415/Antimicrobial_resistance_virulence_factors_and_genetic_lineages_of_hospital_onset_methicillin_resistant_Staphylococcus_aureus_isolates_detected_in_a_hospital_in_Zaragoza_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0213-005X(15)00064-6 DB - PRIME DP - Unbound Medicine ER -