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Determination of genotoxic effects of Imazethapyr herbicide in Allium cepa root cells by mitotic activity, chromosome aberration, and comet assay.
Pestic Biochem Physiol. 2015 Feb; 118:38-42.PB

Abstract

Imazethapyr (IM) is an imidazolinone herbicide that is currently used for broad-spectrum weed control in soybean and other legume crops. In this study, cytotoxic and genotoxic effects of IM were investigated by using mitotic index (MI), mitotic phases, chromosomal abnormalities (CAs) and DNA damage on the root meristem cells of Allium cepa. In Allium root growth inhibition test, EC50 value was determined as 20 ppm, and 0.5xEC50, EC50 and 2xEC50 concentrations of IM herbicide were introduced to onion tuber roots. Distilled water and methyl methane sulfonate (MMS, 10 mg/L) were used as a negative and positive control, respectively. As A. cepa cell cycle is 24 hours, so, application process was carried out for 24, 48, 72 and 96 hours. All the applied doses decreased MIs compared to control group and these declines were found to be statistically meaningful. Analysis of the chromosomes showed that 10 ppm IM except for 48 h induced CAs but 40 ppm IM except for 72 h decreased CAs. DNA damage was found significantly higher in 20 and 40 ppm of IM compared to the control in comet assay. These results indicated that IM herbicide exhibits cytotoxic activity but not genotoxic activity (except 10 ppm) and induced DNA damage in a dose dependent manner in A. cepa root meristematic cells.

Authors+Show Affiliations

Faculty of Arts and Sciences, Molecular Biology and Genetics Department, Uşak University, Uşak 64300, Turkey. Electronic address: recep.liman@usak.edu.tr.Faculty of Science and Literatures, Molecular Biology and Genetics Department, Afyon Kocatepe University, Afyonkarahisar 03200, Turkey.Faculty of Science and Literatures, Molecular Biology and Genetics Department, Afyon Kocatepe University, Afyonkarahisar 03200, Turkey.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25752428

Citation

Liman, Recep, et al. "Determination of Genotoxic Effects of Imazethapyr Herbicide in Allium Cepa Root Cells By Mitotic Activity, Chromosome Aberration, and Comet Assay." Pesticide Biochemistry and Physiology, vol. 118, 2015, pp. 38-42.
Liman R, Ciğerci İH, Öztürk NS. Determination of genotoxic effects of Imazethapyr herbicide in Allium cepa root cells by mitotic activity, chromosome aberration, and comet assay. Pestic Biochem Physiol. 2015;118:38-42.
Liman, R., Ciğerci, İ. H., & Öztürk, N. S. (2015). Determination of genotoxic effects of Imazethapyr herbicide in Allium cepa root cells by mitotic activity, chromosome aberration, and comet assay. Pesticide Biochemistry and Physiology, 118, 38-42. https://doi.org/10.1016/j.pestbp.2014.11.007
Liman R, Ciğerci İH, Öztürk NS. Determination of Genotoxic Effects of Imazethapyr Herbicide in Allium Cepa Root Cells By Mitotic Activity, Chromosome Aberration, and Comet Assay. Pestic Biochem Physiol. 2015;118:38-42. PubMed PMID: 25752428.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of genotoxic effects of Imazethapyr herbicide in Allium cepa root cells by mitotic activity, chromosome aberration, and comet assay. AU - Liman,Recep, AU - Ciğerci,İbrahim Hakkı, AU - Öztürk,Nur Serap, Y1 - 2014/11/20/ PY - 2014/06/18/received PY - 2014/11/17/revised PY - 2014/11/17/accepted PY - 2015/3/11/entrez PY - 2015/3/11/pubmed PY - 2015/12/15/medline KW - Allium test KW - Chromosome aberration KW - Comet assay KW - DNA damage SP - 38 EP - 42 JF - Pesticide biochemistry and physiology JO - Pestic Biochem Physiol VL - 118 N2 - Imazethapyr (IM) is an imidazolinone herbicide that is currently used for broad-spectrum weed control in soybean and other legume crops. In this study, cytotoxic and genotoxic effects of IM were investigated by using mitotic index (MI), mitotic phases, chromosomal abnormalities (CAs) and DNA damage on the root meristem cells of Allium cepa. In Allium root growth inhibition test, EC50 value was determined as 20 ppm, and 0.5xEC50, EC50 and 2xEC50 concentrations of IM herbicide were introduced to onion tuber roots. Distilled water and methyl methane sulfonate (MMS, 10 mg/L) were used as a negative and positive control, respectively. As A. cepa cell cycle is 24 hours, so, application process was carried out for 24, 48, 72 and 96 hours. All the applied doses decreased MIs compared to control group and these declines were found to be statistically meaningful. Analysis of the chromosomes showed that 10 ppm IM except for 48 h induced CAs but 40 ppm IM except for 72 h decreased CAs. DNA damage was found significantly higher in 20 and 40 ppm of IM compared to the control in comet assay. These results indicated that IM herbicide exhibits cytotoxic activity but not genotoxic activity (except 10 ppm) and induced DNA damage in a dose dependent manner in A. cepa root meristematic cells. SN - 1095-9939 UR - https://www.unboundmedicine.com/medline/citation/25752428/Determination_of_genotoxic_effects_of_Imazethapyr_herbicide_in_Allium_cepa_root_cells_by_mitotic_activity_chromosome_aberration_and_comet_assay_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0048-3575(14)00220-X DB - PRIME DP - Unbound Medicine ER -