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Development and validation of an efficient ultrasound assisted extraction of phenolic compounds from flax (Linum usitatissimum L.) seeds.
Ultrason Sonochem. 2015 Sep; 26:176-185.US

Abstract

Flaxseed accumulates in its seedcoat a macromolecular complex composed of lignan (secoisolariciresinol diglucoside, SDG), flavonol (herbacetin diglucoside, HDG) and hydroxycinnamic acids (p-couramic, caffeic and ferulic acid glucosides). Their antioxidant and/or cancer chemopreventive properties support their interest in human health and therefore, the demand for their extraction. In the present study, ultrasound-assisted extraction (UAE) of flaxseed phenolic compounds was investigated. Scanning Electron Microscopy imaging and histochemical analysis revealed the deep alteration of the seedcoat ultrastructure and the release of the mucilage following ultrasound treatment. Therefore, this method was found to be very efficient for the reduction of mucilage entrapment of flaxseed phenolics. The optimal conditions for UAE phenolic compounds extraction from flaxseeds were found to be: water as solvent supplemented with 0.2N of sodium hydroxide for alkaline hydrolysis of the SDG-HMG complex, an extraction time of 60 min at a temperature of 25°C and an ultrasound frequency of 30 kHz. Under these optimized and validated conditions, highest yields of SDG, HDG and hydroxycinnamic acid glucosides were detected in comparison to other published methods. Therefore, the procedure presented herein is a valuable method for efficient extraction and quantification of the main flaxseed phenolics. Moreover, this UAE is of particular interest within the context of green chemistry in terms of reducing energy consumption and valuation of flaxseed cakes as by-products resulting from the production of flax oil.

Authors+Show Affiliations

Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), UPRES EA 1207, Université d'Orléans, Chartres, France.Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), UPRES EA 1207, Université d'Orléans, Chartres, France.Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), UPRES EA 1207, Université d'Orléans, Chartres, France.Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), UPRES EA 1207, Université d'Orléans, Chartres, France.Conditions Extrêmes et Matériaux: Haute Température et Irradiation (CEMHTI) UPR3079, CNRS Orléans, France.Service de Transformations Chimiques et Pharmaceutiques, ERL CNRS 3193, Le CNAM Paris, France.Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), UPRES EA 1207, Université d'Orléans, Chartres, France.Conditions Extrêmes et Matériaux: Haute Température et Irradiation (CEMHTI) UPR3079, CNRS Orléans, France.Service de Transformations Chimiques et Pharmaceutiques, ERL CNRS 3193, Le CNAM Paris, France.Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), UPRES EA 1207, Université d'Orléans, Chartres, France; Ecole Sciences industrielles et technologies de l'information (SITI), Département Chimie Alimentation Santé Environnement Risque (CASER), Le CNAM Paris, France.Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), UPRES EA 1207, Université d'Orléans, Chartres, France.Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), UPRES EA 1207, Université d'Orléans, Chartres, France. Electronic address: hano@univ-orleans.fr.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

25753491

Citation

Corbin, Cyrielle, et al. "Development and Validation of an Efficient Ultrasound Assisted Extraction of Phenolic Compounds From Flax (Linum Usitatissimum L.) Seeds." Ultrasonics Sonochemistry, vol. 26, 2015, pp. 176-185.
Corbin C, Fidel T, Leclerc EA, et al. Development and validation of an efficient ultrasound assisted extraction of phenolic compounds from flax (Linum usitatissimum L.) seeds. Ultrason Sonochem. 2015;26:176-185.
Corbin, C., Fidel, T., Leclerc, E. A., Barakzoy, E., Sagot, N., Falguiéres, A., Renouard, S., Blondeau, J. P., Ferroud, C., Doussot, J., Lainé, E., & Hano, C. (2015). Development and validation of an efficient ultrasound assisted extraction of phenolic compounds from flax (Linum usitatissimum L.) seeds. Ultrasonics Sonochemistry, 26, 176-185. https://doi.org/10.1016/j.ultsonch.2015.02.008
Corbin C, et al. Development and Validation of an Efficient Ultrasound Assisted Extraction of Phenolic Compounds From Flax (Linum Usitatissimum L.) Seeds. Ultrason Sonochem. 2015;26:176-185. PubMed PMID: 25753491.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development and validation of an efficient ultrasound assisted extraction of phenolic compounds from flax (Linum usitatissimum L.) seeds. AU - Corbin,Cyrielle, AU - Fidel,Thibaud, AU - Leclerc,Emilie A, AU - Barakzoy,Esmatullah, AU - Sagot,Nadine, AU - Falguiéres,Annie, AU - Renouard,Sullivan, AU - Blondeau,Jean-Philippe, AU - Ferroud,Clotilde, AU - Doussot,Joël, AU - Lainé,Eric, AU - Hano,Christophe, Y1 - 2015/02/28/ PY - 2014/11/19/received PY - 2015/02/17/revised PY - 2015/02/24/accepted PY - 2015/3/11/entrez PY - 2015/3/11/pubmed PY - 2016/2/10/medline KW - Flavanol KW - Flaxseed KW - Lignan KW - Phenolic compounds KW - Ultrasound-assisted extraction SP - 176 EP - 185 JF - Ultrasonics sonochemistry JO - Ultrason Sonochem VL - 26 N2 - Flaxseed accumulates in its seedcoat a macromolecular complex composed of lignan (secoisolariciresinol diglucoside, SDG), flavonol (herbacetin diglucoside, HDG) and hydroxycinnamic acids (p-couramic, caffeic and ferulic acid glucosides). Their antioxidant and/or cancer chemopreventive properties support their interest in human health and therefore, the demand for their extraction. In the present study, ultrasound-assisted extraction (UAE) of flaxseed phenolic compounds was investigated. Scanning Electron Microscopy imaging and histochemical analysis revealed the deep alteration of the seedcoat ultrastructure and the release of the mucilage following ultrasound treatment. Therefore, this method was found to be very efficient for the reduction of mucilage entrapment of flaxseed phenolics. The optimal conditions for UAE phenolic compounds extraction from flaxseeds were found to be: water as solvent supplemented with 0.2N of sodium hydroxide for alkaline hydrolysis of the SDG-HMG complex, an extraction time of 60 min at a temperature of 25°C and an ultrasound frequency of 30 kHz. Under these optimized and validated conditions, highest yields of SDG, HDG and hydroxycinnamic acid glucosides were detected in comparison to other published methods. Therefore, the procedure presented herein is a valuable method for efficient extraction and quantification of the main flaxseed phenolics. Moreover, this UAE is of particular interest within the context of green chemistry in terms of reducing energy consumption and valuation of flaxseed cakes as by-products resulting from the production of flax oil. SN - 1873-2828 UR - https://www.unboundmedicine.com/medline/citation/25753491/Development_and_validation_of_an_efficient_ultrasound_assisted_extraction_of_phenolic_compounds_from_flax__Linum_usitatissimum_L___seeds_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1350-4177(15)00040-1 DB - PRIME DP - Unbound Medicine ER -