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Transdifferentiation of Human Hair Follicle Mesenchymal Stem Cells into Red Blood Cells by OCT4.
Stem Cells Int. 2015; 2015:389628.SC

Abstract

Shortage of red blood cells (RBCs, erythrocytes) can have potentially life-threatening consequences for rare or unusual blood type patients with massive blood loss resulting from various conditions. Erythrocytes have been derived from human pluripotent stem cells (PSCs), but the risk of potential tumorigenicity cannot be ignored, and a majority of these cells produced from PSCs express embryonic ε- and fetal γ-globins with little or no adult β-globin and remain nucleated. Here we report a method to generate erythrocytes from human hair follicle mesenchymal stem cells (hHFMSCs) by enforcing OCT4 gene expression and cytokine stimulation. Cells generated from hHFMSCs expressed mainly the adult β-globin chain with minimum level of the fetal γ-globin chain. Furthermore, these cells also underwent multiple maturation events and formed enucleated erythrocytes with a biconcave disc shape. Gene expression analyses showed that OCT4 regulated the expression of genes associated with both pluripotency and erythroid development during hHFMSC transdifferentiation toward erythroid cells. These findings show that mature erythrocytes can be generated from adult somatic cells, which may serve as an alternative source of RBCs for potential autologous transfusion.

Authors+Show Affiliations

The Key Laboratory of Pathobiology, Ministry of Education, Department of Pathology, College of Basic Medical Sciences, Jilin University, 126 Xinmin Avenue, Changchun 130021, China.Advanced Cell Technology, 33 Locke Drive, Marlborough, MA 01752, USA.The Key Laboratory of Pathobiology, Ministry of Education, Department of Pathology, College of Basic Medical Sciences, Jilin University, 126 Xinmin Avenue, Changchun 130021, China.Key Laboratory of Biotechnology and Pharmaceutical Engineering, College of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325000, China.The Key Laboratory of Pathobiology, Ministry of Education, Department of Pathology, College of Basic Medical Sciences, Jilin University, 126 Xinmin Avenue, Changchun 130021, China.The Key Laboratory of Pathobiology, Ministry of Education, Department of Pathology, College of Basic Medical Sciences, Jilin University, 126 Xinmin Avenue, Changchun 130021, China.Morphology Center of Hematology Department, Cancer Center, the First Clinical Hospital of Jilin University, Changchun 130000, China.The Key Laboratory of Pathobiology, Ministry of Education, Department of Pathology, College of Basic Medical Sciences, Jilin University, 126 Xinmin Avenue, Changchun 130021, China.The Key Laboratory of Pathobiology, Ministry of Education, Department of Pathology, College of Basic Medical Sciences, Jilin University, 126 Xinmin Avenue, Changchun 130021, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25755671

Citation

Liu, Zhijing, et al. "Transdifferentiation of Human Hair Follicle Mesenchymal Stem Cells Into Red Blood Cells By OCT4." Stem Cells International, vol. 2015, 2015, p. 389628.
Liu Z, Lu SJ, Lu Y, et al. Transdifferentiation of Human Hair Follicle Mesenchymal Stem Cells into Red Blood Cells by OCT4. Stem Cells Int. 2015;2015:389628.
Liu, Z., Lu, S. J., Lu, Y., Tan, X., Zhang, X., Yang, M., Zhang, F., Li, Y., & Quan, C. (2015). Transdifferentiation of Human Hair Follicle Mesenchymal Stem Cells into Red Blood Cells by OCT4. Stem Cells International, 2015, 389628. https://doi.org/10.1155/2015/389628
Liu Z, et al. Transdifferentiation of Human Hair Follicle Mesenchymal Stem Cells Into Red Blood Cells By OCT4. Stem Cells Int. 2015;2015:389628. PubMed PMID: 25755671.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transdifferentiation of Human Hair Follicle Mesenchymal Stem Cells into Red Blood Cells by OCT4. AU - Liu,Zhijing, AU - Lu,Shi-Jiang, AU - Lu,Yan, AU - Tan,Xiaohua, AU - Zhang,Xiaowei, AU - Yang,Minlan, AU - Zhang,Fuming, AU - Li,Yulin, AU - Quan,Chengshi, Y1 - 2015/02/09/ PY - 2014/10/20/received PY - 2015/01/09/revised PY - 2015/01/12/accepted PY - 2015/3/11/entrez PY - 2015/3/11/pubmed PY - 2015/3/11/medline SP - 389628 EP - 389628 JF - Stem cells international JO - Stem Cells Int VL - 2015 N2 - Shortage of red blood cells (RBCs, erythrocytes) can have potentially life-threatening consequences for rare or unusual blood type patients with massive blood loss resulting from various conditions. Erythrocytes have been derived from human pluripotent stem cells (PSCs), but the risk of potential tumorigenicity cannot be ignored, and a majority of these cells produced from PSCs express embryonic ε- and fetal γ-globins with little or no adult β-globin and remain nucleated. Here we report a method to generate erythrocytes from human hair follicle mesenchymal stem cells (hHFMSCs) by enforcing OCT4 gene expression and cytokine stimulation. Cells generated from hHFMSCs expressed mainly the adult β-globin chain with minimum level of the fetal γ-globin chain. Furthermore, these cells also underwent multiple maturation events and formed enucleated erythrocytes with a biconcave disc shape. Gene expression analyses showed that OCT4 regulated the expression of genes associated with both pluripotency and erythroid development during hHFMSC transdifferentiation toward erythroid cells. These findings show that mature erythrocytes can be generated from adult somatic cells, which may serve as an alternative source of RBCs for potential autologous transfusion. SN - 1687-966X UR - https://www.unboundmedicine.com/medline/citation/25755671/Transdifferentiation_of_Human_Hair_Follicle_Mesenchymal_Stem_Cells_into_Red_Blood_Cells_by_OCT4_ L2 - https://doi.org/10.1155/2015/389628 DB - PRIME DP - Unbound Medicine ER -
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