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Characterization of methicillin-resistant and -susceptible staphylococcal isolates from bovine milk in northwestern china.
PLoS One. 2015; 10(3):e0116699.Plos

Abstract

Emergence of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant coagulase-negative staphylococci (MR-CoNS) in bovine milk is a major public health concern. The primary purpose of this research was to determine molecular genetic characteristics and antibiotic resistance of staphylococcal isolates recovered from milk of mastitic cows in the Shaanxi Province in Northwestern China. One hundred and thirteen methicillin-susceptible Staphylococcus aureus (MSSA), one mecA-positive and phenotype-positive MRSA, seven mecA- and mecC- negative but phenotype-positive MRSA and two MR-CoNS including one oxacillin-susceptible mecA-positive Staphylococcus haemolyticus (OS-MRSH) and one mecA-positive and methicillin-resistant Staphylococcus epidermidis (MRSE) isolates were recovered from 214 quarter milk samples on 4 dairy farms. All above 123 isolates were subjected to antibiotic resistance profiling. S. aureus isolates were also genotyped using the spa typing and the multilocus sequence typing (MLST). Eight MRSA and 2 MR-CoNS isolates were additionally tested for SCCmec types. Resistance was common among isolates against ampicillin or penicillin (80.5%), kanamycin (68.3%), gentamicin (67.5%), tetracycline (43.9%) and chloramphenicol (30.1%). However, no isolate was resistant to vancomycin or teicoplanin. Twenty, 29 and 58 isolates showed resistance to 1, 2 or more than 2 antibiotics, respectively. The predominant multidrug resistance profile was penicillin/ampicillin/kanamycin/gentamicin/tetracycline (46 isolates). Most S. aureus isolates belonged to spa types t524 (n = 63), t11772 (a new type, n = 31) and t4207 (n = 15). At the same time, MLST types ST71 (n = 67) and ST2738 (a new type, n = 45) were identified as dominant sequence types. The mecA-positive and phenotype-positive MRSA isolate had a composite genotype t524-ST71-SCCmecIVa, while 7 mecA-negative but phenotype-positive MRSA isolates were all t524-ST71. The OS-MRSH isolate contained a type V SCCmec cassette, while the MRSE isolate possessed a non-typeable SCCmec. The spa-MLST types t11772-ST2738 (n = 27), t11807-ST2683 (n = 4) and t11771-ST2738 (n = 3) were newly identified genotypes of S. aureus. These new genotypes and multidrug-resistant staphylococci could pose additional threat to animal and human health.

Authors+Show Affiliations

College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, People's Republic of China.College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, People's Republic of China.College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, People's Republic of China.College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, People's Republic of China.College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, People's Republic of China; Department of Animal Science, McGill University, 21,111 Lakeshore, Ste. Anne de Bellevue, Quebec, H9X 3V9, Canada.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25756992

Citation

Li, Longping, et al. "Characterization of Methicillin-resistant and -susceptible Staphylococcal Isolates From Bovine Milk in Northwestern China." PloS One, vol. 10, no. 3, 2015, pp. e0116699.
Li L, Zhou L, Wang L, et al. Characterization of methicillin-resistant and -susceptible staphylococcal isolates from bovine milk in northwestern china. PLoS One. 2015;10(3):e0116699.
Li, L., Zhou, L., Wang, L., Xue, H., & Zhao, X. (2015). Characterization of methicillin-resistant and -susceptible staphylococcal isolates from bovine milk in northwestern china. PloS One, 10(3), e0116699. https://doi.org/10.1371/journal.pone.0116699
Li L, et al. Characterization of Methicillin-resistant and -susceptible Staphylococcal Isolates From Bovine Milk in Northwestern China. PLoS One. 2015;10(3):e0116699. PubMed PMID: 25756992.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of methicillin-resistant and -susceptible staphylococcal isolates from bovine milk in northwestern china. AU - Li,Longping, AU - Zhou,Luoxiong, AU - Wang,Lihong, AU - Xue,Huping, AU - Zhao,Xin, Y1 - 2015/03/10/ PY - 2014/08/07/received PY - 2014/12/14/accepted PY - 2015/3/11/entrez PY - 2015/3/11/pubmed PY - 2016/1/14/medline SP - e0116699 EP - e0116699 JF - PloS one JO - PLoS One VL - 10 IS - 3 N2 - Emergence of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant coagulase-negative staphylococci (MR-CoNS) in bovine milk is a major public health concern. The primary purpose of this research was to determine molecular genetic characteristics and antibiotic resistance of staphylococcal isolates recovered from milk of mastitic cows in the Shaanxi Province in Northwestern China. One hundred and thirteen methicillin-susceptible Staphylococcus aureus (MSSA), one mecA-positive and phenotype-positive MRSA, seven mecA- and mecC- negative but phenotype-positive MRSA and two MR-CoNS including one oxacillin-susceptible mecA-positive Staphylococcus haemolyticus (OS-MRSH) and one mecA-positive and methicillin-resistant Staphylococcus epidermidis (MRSE) isolates were recovered from 214 quarter milk samples on 4 dairy farms. All above 123 isolates were subjected to antibiotic resistance profiling. S. aureus isolates were also genotyped using the spa typing and the multilocus sequence typing (MLST). Eight MRSA and 2 MR-CoNS isolates were additionally tested for SCCmec types. Resistance was common among isolates against ampicillin or penicillin (80.5%), kanamycin (68.3%), gentamicin (67.5%), tetracycline (43.9%) and chloramphenicol (30.1%). However, no isolate was resistant to vancomycin or teicoplanin. Twenty, 29 and 58 isolates showed resistance to 1, 2 or more than 2 antibiotics, respectively. The predominant multidrug resistance profile was penicillin/ampicillin/kanamycin/gentamicin/tetracycline (46 isolates). Most S. aureus isolates belonged to spa types t524 (n = 63), t11772 (a new type, n = 31) and t4207 (n = 15). At the same time, MLST types ST71 (n = 67) and ST2738 (a new type, n = 45) were identified as dominant sequence types. The mecA-positive and phenotype-positive MRSA isolate had a composite genotype t524-ST71-SCCmecIVa, while 7 mecA-negative but phenotype-positive MRSA isolates were all t524-ST71. The OS-MRSH isolate contained a type V SCCmec cassette, while the MRSE isolate possessed a non-typeable SCCmec. The spa-MLST types t11772-ST2738 (n = 27), t11807-ST2683 (n = 4) and t11771-ST2738 (n = 3) were newly identified genotypes of S. aureus. These new genotypes and multidrug-resistant staphylococci could pose additional threat to animal and human health. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/25756992/Characterization_of_methicillin_resistant_and__susceptible_staphylococcal_isolates_from_bovine_milk_in_northwestern_china_ L2 - https://dx.plos.org/10.1371/journal.pone.0116699 DB - PRIME DP - Unbound Medicine ER -