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Systemic and mucosal immunity in mice elicited by a single immunization with human adenovirus type 5 or 41 vector-based vaccines carrying the spike protein of Middle East respiratory syndrome coronavirus.
Immunology. 2015 Aug; 145(4):476-84.I

Abstract

An ideal vaccine against mucosal pathogens such as Middle East respiratory syndrome coronavirus (MERS-CoV) should confer sustained, protective immunity at both systemic and mucosal levels. Here, we evaluated the in vivo systemic and mucosal antigen-specific immune responses induced by a single intramuscular or intragastric administration of recombinant adenoviral type 5 (Ad5) or type 41 (Ad41) -based vaccines expressing the MERS-CoV spike (S) protein. Intragastric administration of either Ad5-S or Ad41-S induced antigen-specific IgG and neutralizing antibody in serum; however, antigen-specific T-cell responses were not detected. In contrast, after a single intramuscular dose of Ad5-S or Ad41-S, functional antigen-specific T-cell responses were elicited in the spleen and pulmonary lymphocytes of the mice, which persisted for several months. Both rAd-based vaccines administered intramuscularly induced systemic humoral immune responses (neutralizing IgG antibodies). Our results show that a single dose of Ad5-S- or Ad41-S-based vaccines represents an appealing strategy for the control of MERS-CoV infection and transmission.

Authors+Show Affiliations

Key Laboratory of Medical Virology, Ministry of Health; National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China.Key Laboratory of Medical Virology, Ministry of Health; National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China.Key Laboratory of Medical Virology, Ministry of Health; National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China.Key Laboratory of Medical Virology, Ministry of Health; National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China. Department of Pathogenic Biology, Hebei Medical University, Shijiazhuang, China.Key Laboratory of Medical Virology, Ministry of Health; National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China.Key Laboratory of Medical Virology, Ministry of Health; National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China.Key Laboratory of Medical Virology, Ministry of Health; National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China.Key Laboratory of Medical Virology, Ministry of Health; National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China.Key Laboratory of Medical Virology, Ministry of Health; National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25762305

Citation

Guo, Xiaojuan, et al. "Systemic and Mucosal Immunity in Mice Elicited By a Single Immunization With Human Adenovirus Type 5 or 41 Vector-based Vaccines Carrying the Spike Protein of Middle East Respiratory Syndrome Coronavirus." Immunology, vol. 145, no. 4, 2015, pp. 476-84.
Guo X, Deng Y, Chen H, et al. Systemic and mucosal immunity in mice elicited by a single immunization with human adenovirus type 5 or 41 vector-based vaccines carrying the spike protein of Middle East respiratory syndrome coronavirus. Immunology. 2015;145(4):476-84.
Guo, X., Deng, Y., Chen, H., Lan, J., Wang, W., Zou, X., Hung, T., Lu, Z., & Tan, W. (2015). Systemic and mucosal immunity in mice elicited by a single immunization with human adenovirus type 5 or 41 vector-based vaccines carrying the spike protein of Middle East respiratory syndrome coronavirus. Immunology, 145(4), 476-84. https://doi.org/10.1111/imm.12462
Guo X, et al. Systemic and Mucosal Immunity in Mice Elicited By a Single Immunization With Human Adenovirus Type 5 or 41 Vector-based Vaccines Carrying the Spike Protein of Middle East Respiratory Syndrome Coronavirus. Immunology. 2015;145(4):476-84. PubMed PMID: 25762305.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Systemic and mucosal immunity in mice elicited by a single immunization with human adenovirus type 5 or 41 vector-based vaccines carrying the spike protein of Middle East respiratory syndrome coronavirus. AU - Guo,Xiaojuan, AU - Deng,Yao, AU - Chen,Hong, AU - Lan,Jiaming, AU - Wang,Wen, AU - Zou,Xiaohui, AU - Hung,Tao, AU - Lu,Zhuozhuang, AU - Tan,Wenjie, Y1 - 2015/04/21/ PY - 2014/11/26/received PY - 2015/03/02/revised PY - 2015/03/09/accepted PY - 2015/3/13/entrez PY - 2015/3/13/pubmed PY - 2015/9/19/medline KW - Middle East respiratory syndrome coronavirus KW - adenoviral vector KW - immunity KW - spike protein KW - vaccine SP - 476 EP - 84 JF - Immunology JO - Immunology VL - 145 IS - 4 N2 - An ideal vaccine against mucosal pathogens such as Middle East respiratory syndrome coronavirus (MERS-CoV) should confer sustained, protective immunity at both systemic and mucosal levels. Here, we evaluated the in vivo systemic and mucosal antigen-specific immune responses induced by a single intramuscular or intragastric administration of recombinant adenoviral type 5 (Ad5) or type 41 (Ad41) -based vaccines expressing the MERS-CoV spike (S) protein. Intragastric administration of either Ad5-S or Ad41-S induced antigen-specific IgG and neutralizing antibody in serum; however, antigen-specific T-cell responses were not detected. In contrast, after a single intramuscular dose of Ad5-S or Ad41-S, functional antigen-specific T-cell responses were elicited in the spleen and pulmonary lymphocytes of the mice, which persisted for several months. Both rAd-based vaccines administered intramuscularly induced systemic humoral immune responses (neutralizing IgG antibodies). Our results show that a single dose of Ad5-S- or Ad41-S-based vaccines represents an appealing strategy for the control of MERS-CoV infection and transmission. SN - 1365-2567 UR - https://www.unboundmedicine.com/medline/citation/25762305/Systemic_and_mucosal_immunity_in_mice_elicited_by_a_single_immunization_with_human_adenovirus_type_5_or_41_vector_based_vaccines_carrying_the_spike_protein_of_Middle_East_respiratory_syndrome_coronavirus_ L2 - https://doi.org/10.1111/imm.12462 DB - PRIME DP - Unbound Medicine ER -