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Rolling-circle amplification detection of thrombin using surface-enhanced Raman spectroscopy with core-shell nanoparticle probe.
Chemistry. 2015 Apr 27; 21(18):6817-22.C

Abstract

An ultrasensitive surface-enhanced Raman spectroscopy (SERS) sensor based on rolling-circle amplification (RCA)-increased "hot-spot" was developed for the detection of thrombin. The sensor contains a SERS gold nanoparticle@Raman label@SiO2 core-shell nanoparticle probe in which the Raman reporter molecules are sandwiched between a gold nanoparticle core and a thin silica shell by a layer-by-layer method. Thrombin aptamer sequences were immobilized onto the magnetic beads (MBs) through hybridization with their complementary strand. In the presence of thrombin, the aptamer sequence was released; this allowed the remaining single-stranded DNA (ssDNA) to act as primer and initiate in situ RCA reaction to produce long ssDNAs. Then, a large number of SERS probes were attached on the long ssDNA templates, causing thousands of SERS probes to be involved in each biomolecular recognition event. This SERS method achieved the detection of thrombin in the range from 1.0×10(-12) to 1.0×10(-8) M and a detection limit of 4.2×10(-13) M, and showed good performance in real serum samples.

Authors+Show Affiliations

Shandong Provincial Key Laboratory of Detection Technology of Tumor Markers, School of Chemistry and Chemical Engineering, Linyi University, Linyi 276005 (P. R. China). xuemei_li@yeah.net.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25766032

Citation

Li, Xuemei, et al. "Rolling-circle Amplification Detection of Thrombin Using Surface-enhanced Raman Spectroscopy With Core-shell Nanoparticle Probe." Chemistry (Weinheim an Der Bergstrasse, Germany), vol. 21, no. 18, 2015, pp. 6817-22.
Li X, Wang L, Li C. Rolling-circle amplification detection of thrombin using surface-enhanced Raman spectroscopy with core-shell nanoparticle probe. Chemistry. 2015;21(18):6817-22.
Li, X., Wang, L., & Li, C. (2015). Rolling-circle amplification detection of thrombin using surface-enhanced Raman spectroscopy with core-shell nanoparticle probe. Chemistry (Weinheim an Der Bergstrasse, Germany), 21(18), 6817-22. https://doi.org/10.1002/chem.201405884
Li X, Wang L, Li C. Rolling-circle Amplification Detection of Thrombin Using Surface-enhanced Raman Spectroscopy With Core-shell Nanoparticle Probe. Chemistry. 2015 Apr 27;21(18):6817-22. PubMed PMID: 25766032.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rolling-circle amplification detection of thrombin using surface-enhanced Raman spectroscopy with core-shell nanoparticle probe. AU - Li,Xuemei, AU - Wang,Linlin, AU - Li,Chunxiang, Y1 - 2015/03/12/ PY - 2014/10/30/received PY - 2015/02/09/revised PY - 2015/3/14/entrez PY - 2015/3/15/pubmed PY - 2016/1/13/medline KW - Raman spectroscopy KW - analytical methods KW - nanoparticles KW - rolling-circle amplification KW - thrombin SP - 6817 EP - 22 JF - Chemistry (Weinheim an der Bergstrasse, Germany) JO - Chemistry VL - 21 IS - 18 N2 - An ultrasensitive surface-enhanced Raman spectroscopy (SERS) sensor based on rolling-circle amplification (RCA)-increased "hot-spot" was developed for the detection of thrombin. The sensor contains a SERS gold nanoparticle@Raman label@SiO2 core-shell nanoparticle probe in which the Raman reporter molecules are sandwiched between a gold nanoparticle core and a thin silica shell by a layer-by-layer method. Thrombin aptamer sequences were immobilized onto the magnetic beads (MBs) through hybridization with their complementary strand. In the presence of thrombin, the aptamer sequence was released; this allowed the remaining single-stranded DNA (ssDNA) to act as primer and initiate in situ RCA reaction to produce long ssDNAs. Then, a large number of SERS probes were attached on the long ssDNA templates, causing thousands of SERS probes to be involved in each biomolecular recognition event. This SERS method achieved the detection of thrombin in the range from 1.0×10(-12) to 1.0×10(-8) M and a detection limit of 4.2×10(-13) M, and showed good performance in real serum samples. SN - 1521-3765 UR - https://www.unboundmedicine.com/medline/citation/25766032/Rolling_circle_amplification_detection_of_thrombin_using_surface_enhanced_Raman_spectroscopy_with_core_shell_nanoparticle_probe_ DB - PRIME DP - Unbound Medicine ER -