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Metabolism of gamma-linolenic acid in primary cultures of rat hepatocytes and in Hep G2 cells.
J Nutr Sci Vitaminol (Tokyo). 1989 Dec; 35(6):597-611.JN

Abstract

Incorporation and metabolism of gamma-linolenic acid (GLA) in both rat hepatocytes and Hep G2 cells were compared to those of oleic (OA), linoleic (LA), alpha-linolenic (LLA), and dihomo-gamma-linolenic (DGLA) acids. The incorporation of GLA into both types of cells was higher than LLA and DGLA, but lower than OA and LA. It was efficiently converted into DGLA in both types of cells and increased the concentration of DGLA. LLA was converted to a small amount of C20:4 (n-3) only in Hep G2 cells. Incubation with LA, GLA, LLA, and DGLA did not increase the concentration of arachidonic acid (AA) in both types of cells. LA. GLA, LLA, and their metabolites were incorporated into phosphatidylcholine, but only GLA and its metabolite, DGLA, were also incorporated into phosphatidylethanolamine, phosphatidylserine, and phosphatidylinositol. The coexistence of GLA and LLA during their catabolism diminished the amounts of respective metabolite in Hep G2 cells. The presence of GLA inhibited completely the formation of C20:4(n-3) from LLA. The results indicate that GLA is more effective in raising the ratio of DGLA/AA. Also, polyunsaturated fatty acids of n-3 and n-6 series have competitively catabolized in both types of hepatocytes.

Authors+Show Affiliations

Institute of Environmental Science for Human Life, Ochanomizu University, Tokyo, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2576804

Citation

Fujiyama-Fujiwara, Y, et al. "Metabolism of Gamma-linolenic Acid in Primary Cultures of Rat Hepatocytes and in Hep G2 Cells." Journal of Nutritional Science and Vitaminology, vol. 35, no. 6, 1989, pp. 597-611.
Fujiyama-Fujiwara Y, Ohmori C, Igarashi O. Metabolism of gamma-linolenic acid in primary cultures of rat hepatocytes and in Hep G2 cells. J Nutr Sci Vitaminol (Tokyo). 1989;35(6):597-611.
Fujiyama-Fujiwara, Y., Ohmori, C., & Igarashi, O. (1989). Metabolism of gamma-linolenic acid in primary cultures of rat hepatocytes and in Hep G2 cells. Journal of Nutritional Science and Vitaminology, 35(6), 597-611.
Fujiyama-Fujiwara Y, Ohmori C, Igarashi O. Metabolism of Gamma-linolenic Acid in Primary Cultures of Rat Hepatocytes and in Hep G2 Cells. J Nutr Sci Vitaminol (Tokyo). 1989;35(6):597-611. PubMed PMID: 2576804.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Metabolism of gamma-linolenic acid in primary cultures of rat hepatocytes and in Hep G2 cells. AU - Fujiyama-Fujiwara,Y, AU - Ohmori,C, AU - Igarashi,O, PY - 1989/12/1/pubmed PY - 1989/12/1/medline PY - 1989/12/1/entrez SP - 597 EP - 611 JF - Journal of nutritional science and vitaminology JO - J Nutr Sci Vitaminol (Tokyo) VL - 35 IS - 6 N2 - Incorporation and metabolism of gamma-linolenic acid (GLA) in both rat hepatocytes and Hep G2 cells were compared to those of oleic (OA), linoleic (LA), alpha-linolenic (LLA), and dihomo-gamma-linolenic (DGLA) acids. The incorporation of GLA into both types of cells was higher than LLA and DGLA, but lower than OA and LA. It was efficiently converted into DGLA in both types of cells and increased the concentration of DGLA. LLA was converted to a small amount of C20:4 (n-3) only in Hep G2 cells. Incubation with LA, GLA, LLA, and DGLA did not increase the concentration of arachidonic acid (AA) in both types of cells. LA. GLA, LLA, and their metabolites were incorporated into phosphatidylcholine, but only GLA and its metabolite, DGLA, were also incorporated into phosphatidylethanolamine, phosphatidylserine, and phosphatidylinositol. The coexistence of GLA and LLA during their catabolism diminished the amounts of respective metabolite in Hep G2 cells. The presence of GLA inhibited completely the formation of C20:4(n-3) from LLA. The results indicate that GLA is more effective in raising the ratio of DGLA/AA. Also, polyunsaturated fatty acids of n-3 and n-6 series have competitively catabolized in both types of hepatocytes. SN - 0301-4800 UR - https://www.unboundmedicine.com/medline/citation/2576804/Metabolism_of_gamma_linolenic_acid_in_primary_cultures_of_rat_hepatocytes_and_in_Hep_G2_cells_ L2 - https://medlineplus.gov/livercancer.html DB - PRIME DP - Unbound Medicine ER -