Tags

Type your tag names separated by a space and hit enter

Evidence of active methanogen communities in shallow sediments of the sonora margin cold seeps.
Appl Environ Microbiol. 2015 May 15; 81(10):3451-9.AE

Abstract

In the Sonora Margin cold seep ecosystems (Gulf of California), sediments underlying microbial mats harbor high biogenic methane concentrations, fueling various microbial communities, such as abundant lineages of anaerobic methanotrophs (ANME). However, the biodiversity, distribution, and metabolism of the microorganisms producing this methane remain poorly understood. In this study, measurements of methanogenesis using radiolabeled dimethylamine, bicarbonate, and acetate showed that biogenic methane production in these sediments was mainly dominated by methylotrophic methanogenesis, while the proportion of autotrophic methanogenesis increased with depth. Congruently, methane production and methanogenic Archaea were detected in culture enrichments amended with trimethylamine and bicarbonate. Analyses of denaturing gradient gel electrophoresis (DGGE) fingerprinting and reverse-transcribed PCR-amplified 16S rRNA sequences retrieved from these enrichments revealed the presence of active methylotrophic Methanococcoides burtonii relatives and several new autotrophic Methanogenium lineages, confirming the cooccurrence of Methanosarcinales and Methanomicrobiales methanogens with abundant ANME populations in the sediments of the Sonora Margin cold seeps.

Authors+Show Affiliations

Ifremer, Laboratoire de Microbiologie des Environnements Extrêmes, UMR6197, Technopôle Brest Iroise, Plouzané, France Université de Bretagne Occidentale, Laboratoire de Microbiologie des Environnements Extrêmes, UMR6197, Technopôle Brest Iroise, Plouzané, France CNRS, Laboratoire de Microbiologie des Environnements Extrêmes, UMR6197, Technopôle Brest Iroise, Plouzané, France School of Civil Engineering and Geosciences, Newcastle University, Newcastle upon Tyne, United Kingdom avignero@gmail.com.Université de Bretagne Occidentale, Laboratoire de Microbiologie des Environnements Extrêmes, UMR6197, Technopôle Brest Iroise, Plouzané, France CNRS, Laboratoire de Microbiologie des Environnements Extrêmes, UMR6197, Technopôle Brest Iroise, Plouzané, France.Ifremer, Laboratoire de Microbiologie des Environnements Extrêmes, UMR6197, Technopôle Brest Iroise, Plouzané, France Université de Bretagne Occidentale, Laboratoire de Microbiologie des Environnements Extrêmes, UMR6197, Technopôle Brest Iroise, Plouzané, France CNRS, Laboratoire de Microbiologie des Environnements Extrêmes, UMR6197, Technopôle Brest Iroise, Plouzané, France.Ifremer, Laboratoire de Microbiologie des Environnements Extrêmes, UMR6197, Technopôle Brest Iroise, Plouzané, France Université de Bretagne Occidentale, Laboratoire de Microbiologie des Environnements Extrêmes, UMR6197, Technopôle Brest Iroise, Plouzané, France CNRS, Laboratoire de Microbiologie des Environnements Extrêmes, UMR6197, Technopôle Brest Iroise, Plouzané, France School of Earth and Ocean Sciences, Cardiff University, Cardiff, United Kingdom.School of Earth and Ocean Sciences, Cardiff University, Cardiff, United Kingdom.School of Earth and Ocean Sciences, Cardiff University, Cardiff, United Kingdom.Ifremer, Laboratoire de Microbiologie des Environnements Extrêmes, UMR6197, Technopôle Brest Iroise, Plouzané, France Université de Bretagne Occidentale, Laboratoire de Microbiologie des Environnements Extrêmes, UMR6197, Technopôle Brest Iroise, Plouzané, France CNRS, Laboratoire de Microbiologie des Environnements Extrêmes, UMR6197, Technopôle Brest Iroise, Plouzané, France.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25769831

Citation

Vigneron, Adrien, et al. "Evidence of Active Methanogen Communities in Shallow Sediments of the Sonora Margin Cold Seeps." Applied and Environmental Microbiology, vol. 81, no. 10, 2015, pp. 3451-9.
Vigneron A, L'Haridon S, Godfroy A, et al. Evidence of active methanogen communities in shallow sediments of the sonora margin cold seeps. Appl Environ Microbiol. 2015;81(10):3451-9.
Vigneron, A., L'Haridon, S., Godfroy, A., Roussel, E. G., Cragg, B. A., Parkes, R. J., & Toffin, L. (2015). Evidence of active methanogen communities in shallow sediments of the sonora margin cold seeps. Applied and Environmental Microbiology, 81(10), 3451-9. https://doi.org/10.1128/AEM.00147-15
Vigneron A, et al. Evidence of Active Methanogen Communities in Shallow Sediments of the Sonora Margin Cold Seeps. Appl Environ Microbiol. 2015 May 15;81(10):3451-9. PubMed PMID: 25769831.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evidence of active methanogen communities in shallow sediments of the sonora margin cold seeps. AU - Vigneron,Adrien, AU - L'Haridon,Stéphane, AU - Godfroy,Anne, AU - Roussel,Erwan G, AU - Cragg,Barry A, AU - Parkes,R John, AU - Toffin,Laurent, Y1 - 2015/03/13/ PY - 2015/01/19/received PY - 2015/03/05/accepted PY - 2015/3/15/entrez PY - 2015/3/15/pubmed PY - 2016/1/16/medline SP - 3451 EP - 9 JF - Applied and environmental microbiology JO - Appl. Environ. Microbiol. VL - 81 IS - 10 N2 - In the Sonora Margin cold seep ecosystems (Gulf of California), sediments underlying microbial mats harbor high biogenic methane concentrations, fueling various microbial communities, such as abundant lineages of anaerobic methanotrophs (ANME). However, the biodiversity, distribution, and metabolism of the microorganisms producing this methane remain poorly understood. In this study, measurements of methanogenesis using radiolabeled dimethylamine, bicarbonate, and acetate showed that biogenic methane production in these sediments was mainly dominated by methylotrophic methanogenesis, while the proportion of autotrophic methanogenesis increased with depth. Congruently, methane production and methanogenic Archaea were detected in culture enrichments amended with trimethylamine and bicarbonate. Analyses of denaturing gradient gel electrophoresis (DGGE) fingerprinting and reverse-transcribed PCR-amplified 16S rRNA sequences retrieved from these enrichments revealed the presence of active methylotrophic Methanococcoides burtonii relatives and several new autotrophic Methanogenium lineages, confirming the cooccurrence of Methanosarcinales and Methanomicrobiales methanogens with abundant ANME populations in the sediments of the Sonora Margin cold seeps. SN - 1098-5336 UR - https://www.unboundmedicine.com/medline/citation/25769831/Evidence_of_active_methanogen_communities_in_shallow_sediments_of_the_sonora_margin_cold_seeps_ L2 - http://aem.asm.org/cgi/pmidlookup?view=long&pmid=25769831 DB - PRIME DP - Unbound Medicine ER -