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Anamperometric superoxide anion radicalbiosensor based on SOD/PtPd-PDARGO modified electrode.
Talanta. 2015 May; 137:18-24.T

Abstract

In the present work, a high-performance enzyme-based electrochemical sensor for the detection of superoxide anion radical (O2(●-)) is reported. Firstly, we employed a facile approach to synthesize PtPd nanoparticles (PtPd NPs) on chemically reduced graphene oxide (RGO) coated with polydopamine (PDA). The prepared PtPd-PDARGO composite was well characterized by transmission electron microscopy, scanning electron microscopy, Fourier transform infrared spectra, X-ray diffraction, X-ray photoelectron spectroscopy and electrochemical methods. Then the assembled composite was used as a desired electrochemcial interface for superoxide dismutase (SOD) immobilization. Owing to the PDA layer as well as the synergistic effect of PtPd NPs, the fabricated SOD/PtPd-PDARGO sensor exhibited an outstanding sensitivity of 909.7 μA mM(-1) cm(-2) upon O2(●-) in a linear range from 0.016 mM to 0.24 mM (R(2)=0.992), with a low detection limit of 2 μM (S/N=3) and excellent selectivity, good reproducibility as well as favorable long-term stability.

Authors+Show Affiliations

Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai 200237, PR China.Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai 200237, PR China.Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai 200237, PR China.Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai 200237, PR China.Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai 200237, PR China. Electronic address: honglizhao@ecust.edu.cn.Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai 200237, PR China; State Key Laboratory of Bioreactor Engineering, East China University of Scienceand Technology, Shanghai 200237, PR China. Electronic address: minbolan@ecust.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25770601

Citation

Tang, Jie, et al. "Anamperometric Superoxide Anion Radicalbiosensor Based On SOD/PtPd-PDARGO Modified Electrode." Talanta, vol. 137, 2015, pp. 18-24.
Tang J, Zhu X, Niu X, et al. Anamperometric superoxide anion radicalbiosensor based on SOD/PtPd-PDARGO modified electrode. Talanta. 2015;137:18-24.
Tang, J., Zhu, X., Niu, X., Liu, T., Zhao, H., & Lan, M. (2015). Anamperometric superoxide anion radicalbiosensor based on SOD/PtPd-PDARGO modified electrode. Talanta, 137, 18-24. https://doi.org/10.1016/j.talanta.2015.01.009
Tang J, et al. Anamperometric Superoxide Anion Radicalbiosensor Based On SOD/PtPd-PDARGO Modified Electrode. Talanta. 2015;137:18-24. PubMed PMID: 25770601.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anamperometric superoxide anion radicalbiosensor based on SOD/PtPd-PDARGO modified electrode. AU - Tang,Jie, AU - Zhu,Xiang, AU - Niu,Xiangheng, AU - Liu,Tingting, AU - Zhao,Hongli, AU - Lan,Minbo, Y1 - 2015/01/13/ PY - 2014/10/24/received PY - 2015/01/04/revised PY - 2015/01/06/accepted PY - 2015/3/16/entrez PY - 2015/3/17/pubmed PY - 2015/12/15/medline KW - PDARGO KW - PtPd NPs KW - Superoxide anion radical KW - Superoxide dismutase SP - 18 EP - 24 JF - Talanta JO - Talanta VL - 137 N2 - In the present work, a high-performance enzyme-based electrochemical sensor for the detection of superoxide anion radical (O2(●-)) is reported. Firstly, we employed a facile approach to synthesize PtPd nanoparticles (PtPd NPs) on chemically reduced graphene oxide (RGO) coated with polydopamine (PDA). The prepared PtPd-PDARGO composite was well characterized by transmission electron microscopy, scanning electron microscopy, Fourier transform infrared spectra, X-ray diffraction, X-ray photoelectron spectroscopy and electrochemical methods. Then the assembled composite was used as a desired electrochemcial interface for superoxide dismutase (SOD) immobilization. Owing to the PDA layer as well as the synergistic effect of PtPd NPs, the fabricated SOD/PtPd-PDARGO sensor exhibited an outstanding sensitivity of 909.7 μA mM(-1) cm(-2) upon O2(●-) in a linear range from 0.016 mM to 0.24 mM (R(2)=0.992), with a low detection limit of 2 μM (S/N=3) and excellent selectivity, good reproducibility as well as favorable long-term stability. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/25770601/Anamperometric_superoxide_anion_radicalbiosensor_based_on_SOD/PtPd_PDARGO_modified_electrode_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(15)00014-4 DB - PRIME DP - Unbound Medicine ER -