Abstract
A novel label-free strategy for the detection of bacteria was developed by using a specific antimicrobial peptide (AMP)-functionalized quartz crystal microbalance (QCM) electrode. This electrode interface was successfully applied to detect pathogenic Escherichia coli O157:H7 based on the specific affinity between the small synthetic antimicrobial peptide and the bacterial cell of pathogenic E. coli O157:H7. The concentrations of pathogenic E. coli O157:H7 were sensitively measured by the frequency response of the QCM with a detection limit of 0.4 cfu μL(-1). The detection can be fulfilled within 10 min because it does not require germiculture process. On the other hand, if the specific antimicrobial peptides were immobilized on a gold electrode, this label-free strategy can also be performed by electrochemical impedance spectroscopy (EIS). Compared with QCM technique, the EIS measurement gives a lower sensitivity and needs a longer assay time. The combination of antimicrobial peptides with the real-time responses of QCM, as well as electronic read-out monitoring of EIS, may open a new way for the direct detection of bacteria.
TY - JOUR
T1 - Label-free detection of pathogenic bacteria via immobilized antimicrobial peptides.
AU - Dong,Zong-Mu,
AU - Zhao,Guang-Chao,
Y1 - 2015/01/19/
PY - 2014/10/10/received
PY - 2015/01/06/revised
PY - 2015/01/10/accepted
PY - 2015/3/16/entrez
PY - 2015/3/17/pubmed
PY - 2015/12/15/medline
KW - Antimicrobial peptide
KW - Biorecognition
KW - Pathogenic Escherichia coli O157:H7
KW - Quartz crystal microbalance
SP - 55
EP - 61
JF - Talanta
JO - Talanta
VL - 137
N2 - A novel label-free strategy for the detection of bacteria was developed by using a specific antimicrobial peptide (AMP)-functionalized quartz crystal microbalance (QCM) electrode. This electrode interface was successfully applied to detect pathogenic Escherichia coli O157:H7 based on the specific affinity between the small synthetic antimicrobial peptide and the bacterial cell of pathogenic E. coli O157:H7. The concentrations of pathogenic E. coli O157:H7 were sensitively measured by the frequency response of the QCM with a detection limit of 0.4 cfu μL(-1). The detection can be fulfilled within 10 min because it does not require germiculture process. On the other hand, if the specific antimicrobial peptides were immobilized on a gold electrode, this label-free strategy can also be performed by electrochemical impedance spectroscopy (EIS). Compared with QCM technique, the EIS measurement gives a lower sensitivity and needs a longer assay time. The combination of antimicrobial peptides with the real-time responses of QCM, as well as electronic read-out monitoring of EIS, may open a new way for the direct detection of bacteria.
SN - 1873-3573
UR - https://www.unboundmedicine.com/medline/citation/25770606/Label_free_detection_of_pathogenic_bacteria_via_immobilized_antimicrobial_peptides_
DB - PRIME
DP - Unbound Medicine
ER -
