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In vitro glycation of an endothelialized and innervated tissue-engineered skin to screen anti-AGE molecules.
Biomaterials. 2015 May; 51:216-225.B

Abstract

Glycation is one of the major processes responsible for skin aging through induction of the detrimental formation of advanced glycation end-products (AGEs). We developed an innovative tissue-engineered skin combining both a capillary-like and a nerve networks and designed a protocol to induce continuous AGEs formation by a treatment with glyoxal. We determined the optimal concentration of glyoxal to induce AGEs formation identified by carboxymethyl-lysin expression while keeping their toxic effects low. We showed that our tissue-engineered skin cultured for 44 days and treated with 200 μm glyoxal for 31 days displayed high carboxymethyl-lysine expression, which induced a progressively increased alteration of its capillary and nerve networks between 28 and 44 days. Moreover, it produced an epidermal differentiation defect evidenced by the lack of loricrin and filaggrin expression in the epidermis. These effects were almost completely prevented by addition of aminoguanidine 1.5 mm, an anti-glycation compound, and only slightly decreased by alagebrium 500 μm, an AGE-breaker molecule. This tissue-engineered skin model is the first one to combine a capillary and nerve network and to enable a continuous glycation over a long-term culture period. It is a unique tool to investigate the effects of glycation on skin and to screen new molecules that could prevent AGEs formation.

Authors+Show Affiliations

Centre LOEX de l'Université Laval, Centre de recherche du CHU de Québec, 1401, 18e Rue, Québec, QC G1J 1Z4, Canada; Département de Chirurgie, Faculté de Médecine, Université Laval, Québec, QC, Canada.BASF Beauty Care Solutions, 32 Rue Saint Jean de Dieu, Lyon 69007, France.BASF Beauty Care Solutions, 32 Rue Saint Jean de Dieu, Lyon 69007, France.BASF Beauty Care Solutions, 32 Rue Saint Jean de Dieu, Lyon 69007, France.BASF Beauty Care Solutions, 32 Rue Saint Jean de Dieu, Lyon 69007, France.Centre LOEX de l'Université Laval, Centre de recherche du CHU de Québec, 1401, 18e Rue, Québec, QC G1J 1Z4, Canada; Département de Chirurgie, Faculté de Médecine, Université Laval, Québec, QC, Canada. Electronic address: francois.berthod@fmed.ulaval.ca.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25771012

Citation

Cadau, Sébastien, et al. "In Vitro Glycation of an Endothelialized and Innervated Tissue-engineered Skin to Screen anti-AGE Molecules." Biomaterials, vol. 51, 2015, pp. 216-225.
Cadau S, Leoty-Okombi S, Pain S, et al. In vitro glycation of an endothelialized and innervated tissue-engineered skin to screen anti-AGE molecules. Biomaterials. 2015;51:216-225.
Cadau, S., Leoty-Okombi, S., Pain, S., Bechetoille, N., André-Frei, V., & Berthod, F. (2015). In vitro glycation of an endothelialized and innervated tissue-engineered skin to screen anti-AGE molecules. Biomaterials, 51, 216-225. https://doi.org/10.1016/j.biomaterials.2015.01.066
Cadau S, et al. In Vitro Glycation of an Endothelialized and Innervated Tissue-engineered Skin to Screen anti-AGE Molecules. Biomaterials. 2015;51:216-225. PubMed PMID: 25771012.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro glycation of an endothelialized and innervated tissue-engineered skin to screen anti-AGE molecules. AU - Cadau,Sébastien, AU - Leoty-Okombi,Sabrina, AU - Pain,Sabine, AU - Bechetoille,Nicolas, AU - André-Frei,Valérie, AU - Berthod,François, Y1 - 2015/02/19/ PY - 2014/11/05/received PY - 2015/01/12/revised PY - 2015/01/25/accepted PY - 2015/3/16/entrez PY - 2015/3/17/pubmed PY - 2015/12/15/medline KW - Advanced glycation end products KW - Angiogenesis KW - Carboxymethyl lysine KW - Glyoxal KW - Skin SP - 216 EP - 225 JF - Biomaterials JO - Biomaterials VL - 51 N2 - Glycation is one of the major processes responsible for skin aging through induction of the detrimental formation of advanced glycation end-products (AGEs). We developed an innovative tissue-engineered skin combining both a capillary-like and a nerve networks and designed a protocol to induce continuous AGEs formation by a treatment with glyoxal. We determined the optimal concentration of glyoxal to induce AGEs formation identified by carboxymethyl-lysin expression while keeping their toxic effects low. We showed that our tissue-engineered skin cultured for 44 days and treated with 200 μm glyoxal for 31 days displayed high carboxymethyl-lysine expression, which induced a progressively increased alteration of its capillary and nerve networks between 28 and 44 days. Moreover, it produced an epidermal differentiation defect evidenced by the lack of loricrin and filaggrin expression in the epidermis. These effects were almost completely prevented by addition of aminoguanidine 1.5 mm, an anti-glycation compound, and only slightly decreased by alagebrium 500 μm, an AGE-breaker molecule. This tissue-engineered skin model is the first one to combine a capillary and nerve network and to enable a continuous glycation over a long-term culture period. It is a unique tool to investigate the effects of glycation on skin and to screen new molecules that could prevent AGEs formation. SN - 1878-5905 UR - https://www.unboundmedicine.com/medline/citation/25771012/In_vitro_glycation_of_an_endothelialized_and_innervated_tissue_engineered_skin_to_screen_anti_AGE_molecules_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0142-9612(15)00083-6 DB - PRIME DP - Unbound Medicine ER -