Tags

Type your tag names separated by a space and hit enter

Determination of geniposide in adjuvant arthritis rat plasma by ultra-high performance liquid chromatography tandem mass spectrometry method and its application to oral bioavailability and plasma protein binding ability studies.
J Pharm Biomed Anal. 2015 Apr 10; 108:122-8.JP

Abstract

A specific, sensitive and high throughput ultra-high performance liquid chromatography-electrospray ionization tandem mass spectrometric method (UHPLC-ESI-MS/MS) was established and validated to assay geniposide (GE), a promising anti-inflammatory drug, in adjuvant arthritis rat plasma: application to pharmacokinetic and oral bioavailability studies and plasma protein binding ability. Plasma samples were processed by de-proteinised with ice-cold methanol and separated on an ACQUITY UPLC™ HSS C18 column (100 mm × 2.1mm i.d., 1.8 μm particle size) at a gradient flow rate of 0.2 mL/min using acetonitrile-0.1% formic acid in water as mobile phase, and the total run time was 9 min. Mass detection was performed in selected reaction monitoring (SRM) mode with negative electro-spray ionization includes the addition of paeoniflorin (Pae) as an internal standard (IS). The mass transition ion-pair was followed as m/z 387.4 → 122.4 for GE and m/z 479.4 → 449.0 for IS. The calibration curves were linear over the concentration range of 2-50,000 ng/mL with lower limit of quantification of 2 ng/mL. The intra-day and inter-day precisions (RSD, %) of the assay were less than 8.4%, and the accuracy was within ± 6.4% in terms of relative error (RE). Extraction recovery, matrix effect and stability were satisfactory in adjuvant arthritis rat plasma. The UHPLC-ESI-MS/MS method was successfully applied to a pharmacokinetic study of GE after oral administration of depurated GE at 33, 66, 132 mg/kg and intravenous injection at 33, 66, 132 mg/kg in adjuvant arthritis (AA) rats. In addition, it was found that GE has rapid absorption and elimination, low absolute bioavailability, high plasma protein binding ability in AA rats after oral administration within the tested dosage range. It suggested that GE showed slow distribution into the intra- and extracellular space, and the binding rate was not proportionally dependent on plasma concentration of GE when the concentration of GE was below 5.0 μg/mL.

Authors+Show Affiliations

College of Pharmacy, Anhui University of Chinese Medicine, Key Laboratory of Modernized Chinese Medicine in Anhui Province, Hefei, Anhui, China.College of Pharmacy, Anhui University of Chinese Medicine, Key Laboratory of Modernized Chinese Medicine in Anhui Province, Hefei, Anhui, China. Electronic address: hongw@aliyun.com.Anhui Institute for Drug Control, Hefei, Anhui, China.College of Pharmacy, Anhui University of Chinese Medicine, Key Laboratory of Modernized Chinese Medicine in Anhui Province, Hefei, Anhui, China.College of Pharmacy, Anhui University of Chinese Medicine, Key Laboratory of Modernized Chinese Medicine in Anhui Province, Hefei, Anhui, China.College of Pharmacy, Anhui University of Chinese Medicine, Key Laboratory of Modernized Chinese Medicine in Anhui Province, Hefei, Anhui, China.College of Pharmacy, Anhui University of Chinese Medicine, Key Laboratory of Modernized Chinese Medicine in Anhui Province, Hefei, Anhui, China.College of Pharmacy, Anhui University of Chinese Medicine, Key Laboratory of Modernized Chinese Medicine in Anhui Province, Hefei, Anhui, China.College of Pharmacy, Anhui University of Chinese Medicine, Key Laboratory of Modernized Chinese Medicine in Anhui Province, Hefei, Anhui, China.College of Pharmacy, Anhui University of Chinese Medicine, Key Laboratory of Modernized Chinese Medicine in Anhui Province, Hefei, Anhui, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

25771205

Citation

Chen, Jian, et al. "Determination of Geniposide in Adjuvant Arthritis Rat Plasma By Ultra-high Performance Liquid Chromatography Tandem Mass Spectrometry Method and Its Application to Oral Bioavailability and Plasma Protein Binding Ability Studies." Journal of Pharmaceutical and Biomedical Analysis, vol. 108, 2015, pp. 122-8.
Chen J, Wu H, Xu GB, et al. Determination of geniposide in adjuvant arthritis rat plasma by ultra-high performance liquid chromatography tandem mass spectrometry method and its application to oral bioavailability and plasma protein binding ability studies. J Pharm Biomed Anal. 2015;108:122-8.
Chen, J., Wu, H., Xu, G. B., Dai, M. M., Hu, S. L., Sun, L. L., Wang, W., Wang, R., Li, S. P., & Li, G. Q. (2015). Determination of geniposide in adjuvant arthritis rat plasma by ultra-high performance liquid chromatography tandem mass spectrometry method and its application to oral bioavailability and plasma protein binding ability studies. Journal of Pharmaceutical and Biomedical Analysis, 108, 122-8. https://doi.org/10.1016/j.jpba.2015.01.044
Chen J, et al. Determination of Geniposide in Adjuvant Arthritis Rat Plasma By Ultra-high Performance Liquid Chromatography Tandem Mass Spectrometry Method and Its Application to Oral Bioavailability and Plasma Protein Binding Ability Studies. J Pharm Biomed Anal. 2015 Apr 10;108:122-8. PubMed PMID: 25771205.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of geniposide in adjuvant arthritis rat plasma by ultra-high performance liquid chromatography tandem mass spectrometry method and its application to oral bioavailability and plasma protein binding ability studies. AU - Chen,Jian, AU - Wu,Hong, AU - Xu,Guo-Bing, AU - Dai,Miao-Miao, AU - Hu,Shun-Li, AU - Sun,Liang-Liang, AU - Wang,Wei, AU - Wang,Rong, AU - Li,Shu-Pin, AU - Li,Guo-Qiang, Y1 - 2015/02/07/ PY - 2014/12/10/received PY - 2015/01/19/revised PY - 2015/01/20/accepted PY - 2015/3/16/entrez PY - 2015/3/17/pubmed PY - 2015/12/15/medline KW - Absolute oral bioavailability KW - Geniposide KW - Pharmacokinetic KW - Plasma protein binding KW - UHPLC–ESI-MS/MS SP - 122 EP - 8 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 108 N2 - A specific, sensitive and high throughput ultra-high performance liquid chromatography-electrospray ionization tandem mass spectrometric method (UHPLC-ESI-MS/MS) was established and validated to assay geniposide (GE), a promising anti-inflammatory drug, in adjuvant arthritis rat plasma: application to pharmacokinetic and oral bioavailability studies and plasma protein binding ability. Plasma samples were processed by de-proteinised with ice-cold methanol and separated on an ACQUITY UPLC™ HSS C18 column (100 mm × 2.1mm i.d., 1.8 μm particle size) at a gradient flow rate of 0.2 mL/min using acetonitrile-0.1% formic acid in water as mobile phase, and the total run time was 9 min. Mass detection was performed in selected reaction monitoring (SRM) mode with negative electro-spray ionization includes the addition of paeoniflorin (Pae) as an internal standard (IS). The mass transition ion-pair was followed as m/z 387.4 → 122.4 for GE and m/z 479.4 → 449.0 for IS. The calibration curves were linear over the concentration range of 2-50,000 ng/mL with lower limit of quantification of 2 ng/mL. The intra-day and inter-day precisions (RSD, %) of the assay were less than 8.4%, and the accuracy was within ± 6.4% in terms of relative error (RE). Extraction recovery, matrix effect and stability were satisfactory in adjuvant arthritis rat plasma. The UHPLC-ESI-MS/MS method was successfully applied to a pharmacokinetic study of GE after oral administration of depurated GE at 33, 66, 132 mg/kg and intravenous injection at 33, 66, 132 mg/kg in adjuvant arthritis (AA) rats. In addition, it was found that GE has rapid absorption and elimination, low absolute bioavailability, high plasma protein binding ability in AA rats after oral administration within the tested dosage range. It suggested that GE showed slow distribution into the intra- and extracellular space, and the binding rate was not proportionally dependent on plasma concentration of GE when the concentration of GE was below 5.0 μg/mL. SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/25771205/Determination_of_geniposide_in_adjuvant_arthritis_rat_plasma_by_ultra_high_performance_liquid_chromatography_tandem_mass_spectrometry_method_and_its_application_to_oral_bioavailability_and_plasma_protein_binding_ability_studies_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(15)00054-0 DB - PRIME DP - Unbound Medicine ER -