Tags

Type your tag names separated by a space and hit enter

Effect of apoptosis in neural stem cells treated with sevoflurane.
BMC Anesthesiol. 2015; 15:25.BA

Abstract

BACKGROUND

At present, sevoflurane inhalation anesthesia used on infants is well-known. But long-time exposure to inhalation anesthetic could cause neurologic disorder, especially nerve degeneration in infant and developing brain. The central nervous system degeneration of infants could affect the memory and cognitive function. γ-Aminobutyric acid (GABA) is a known inhibitory neurotransmitter in central nervous system. Inhalation anesthetic sevoflurane may activate GABAA receptor to inhibit central nervous system, leading to apoptosis of neural degeneration, cognitive dysfunction in the critical period of brain development.

METHODS

Neural stem cells were derived from Wistar embryos, cultured in vitro. Third generation of neural stem cells were randomly divided into four groups according to cultured suspension: Sevoflurane group (Group S), GABAA receptor antagonists, Bicuculline group (Group B), Sevoflurane + GABAA receptor antagonists, Bicuculline group (Group S + B), dimethyl sulphoxide (DMSO) group (Group D). Group B and Group D did not receive sevoflurane preconditioning. Group S and Group S + B were pretreated with 1 minimum alveolar concentration (MAC) sevoflurane for 0 h, 3 h, 6 h, and 12 h. Group S + B and Group B were pretreated with bicuculline (10 uM). Group D was treated with DMSO (10 uL/mL). After treatments above, all groups were cultured for 48 h. Then we measured the cells viability by Cell Counting Kit (CCK-8) assay, cytotoxicity by Lactate Dehydrogenase (LDH) assay, apoptosis ratio with Annexin V/propidium iodide (PI) staining by flow cytometry, and the expression of GABAAR, anti-apoptotic protein Bcl-2, pro-apoptotic protein Bax and Caspase-3 by western blotting.

RESULTS

After exposing to sevoflurane for 0 h, 3 h, 6 h, and 12 h with 1MAC, we found that cell viability obviously decreased and cytotoxicity increased in time-dependent way. And Annexin V/PI staining indicated increased apoptosis ratio by flow cytometry. The protein level of GABAA receptor, pro-apoptotic protein Bax and apoptosis protein Caspase-3 increased; while anti-apoptotic protein Bcl-2 decreased. And bicuculline could reverse all detrimental results caused by sevoflurane.

CONCLUSION

Sevoflurane can inhibit the central nervous system by activating GABAA, resulting in apoptosis of neural stem cells, thus leading to the NSCs degeneration.

Authors+Show Affiliations

Department of Anesthesiology, Dezhou People's Hospital, Dezhou, Shandong China ; School of Medicine, Shandong University, Ji'nan, Shandong China.Department of Anesthesiology, Shandong Provincial Qianfoshan Hospital, Ji'nan, Shandong China.Department of Anesthesiology, Jiaozhou Central Hospital of Qingdao, Qingdao, Shandong China.Department of Anesthesiology, Dezhou People's Hospital, Dezhou, Shandong China.Department of Emergency, Dezhou People's Hospital, Dezhou, Shandong China.School of Medicine, Shandong University, Ji'nan, Shandong China ; Department of Anesthesiology, Shandong Provincial Qianfoshan Hospital, Ji'nan, Shandong China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25774088

Citation

Qiu, Jianlei, et al. "Effect of Apoptosis in Neural Stem Cells Treated With Sevoflurane." BMC Anesthesiology, vol. 15, 2015, p. 25.
Qiu J, Shi P, Mao W, et al. Effect of apoptosis in neural stem cells treated with sevoflurane. BMC Anesthesiol. 2015;15:25.
Qiu, J., Shi, P., Mao, W., Zhao, Y., Liu, W., & Wang, Y. (2015). Effect of apoptosis in neural stem cells treated with sevoflurane. BMC Anesthesiology, 15, 25. https://doi.org/10.1186/s12871-015-0018-8
Qiu J, et al. Effect of Apoptosis in Neural Stem Cells Treated With Sevoflurane. BMC Anesthesiol. 2015;15:25. PubMed PMID: 25774088.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of apoptosis in neural stem cells treated with sevoflurane. AU - Qiu,Jianlei, AU - Shi,Pengcai, AU - Mao,Wude, AU - Zhao,Yuyi, AU - Liu,Wenshuai, AU - Wang,Yuelan, Y1 - 2015/03/07/ PY - 2014/09/22/received PY - 2015/02/24/accepted PY - 2015/3/17/entrez PY - 2015/3/17/pubmed PY - 2016/3/5/medline KW - Apoptosis KW - Neural Stem Cells KW - Sevoflurane KW - γ-Aminobutyric acid SP - 25 EP - 25 JF - BMC anesthesiology JO - BMC Anesthesiol VL - 15 N2 - BACKGROUND: At present, sevoflurane inhalation anesthesia used on infants is well-known. But long-time exposure to inhalation anesthetic could cause neurologic disorder, especially nerve degeneration in infant and developing brain. The central nervous system degeneration of infants could affect the memory and cognitive function. γ-Aminobutyric acid (GABA) is a known inhibitory neurotransmitter in central nervous system. Inhalation anesthetic sevoflurane may activate GABAA receptor to inhibit central nervous system, leading to apoptosis of neural degeneration, cognitive dysfunction in the critical period of brain development. METHODS: Neural stem cells were derived from Wistar embryos, cultured in vitro. Third generation of neural stem cells were randomly divided into four groups according to cultured suspension: Sevoflurane group (Group S), GABAA receptor antagonists, Bicuculline group (Group B), Sevoflurane + GABAA receptor antagonists, Bicuculline group (Group S + B), dimethyl sulphoxide (DMSO) group (Group D). Group B and Group D did not receive sevoflurane preconditioning. Group S and Group S + B were pretreated with 1 minimum alveolar concentration (MAC) sevoflurane for 0 h, 3 h, 6 h, and 12 h. Group S + B and Group B were pretreated with bicuculline (10 uM). Group D was treated with DMSO (10 uL/mL). After treatments above, all groups were cultured for 48 h. Then we measured the cells viability by Cell Counting Kit (CCK-8) assay, cytotoxicity by Lactate Dehydrogenase (LDH) assay, apoptosis ratio with Annexin V/propidium iodide (PI) staining by flow cytometry, and the expression of GABAAR, anti-apoptotic protein Bcl-2, pro-apoptotic protein Bax and Caspase-3 by western blotting. RESULTS: After exposing to sevoflurane for 0 h, 3 h, 6 h, and 12 h with 1MAC, we found that cell viability obviously decreased and cytotoxicity increased in time-dependent way. And Annexin V/PI staining indicated increased apoptosis ratio by flow cytometry. The protein level of GABAA receptor, pro-apoptotic protein Bax and apoptosis protein Caspase-3 increased; while anti-apoptotic protein Bcl-2 decreased. And bicuculline could reverse all detrimental results caused by sevoflurane. CONCLUSION: Sevoflurane can inhibit the central nervous system by activating GABAA, resulting in apoptosis of neural stem cells, thus leading to the NSCs degeneration. SN - 1471-2253 UR - https://www.unboundmedicine.com/medline/citation/25774088/Effect_of_apoptosis_in_neural_stem_cells_treated_with_sevoflurane_ L2 - https://bmcanesthesiol.biomedcentral.com/articles/10.1186/s12871-015-0018-8 DB - PRIME DP - Unbound Medicine ER -