Tags

Type your tag names separated by a space and hit enter

Soluble ligands for the NKG2D receptor are released during endometriosis and correlate with disease severity.
PLoS One 2015; 10(3):e0119961Plos

Abstract

BACKGROUND

Endometriosis is a benign gynaecological disease. Abundant bulk of evidence suggests that patients with endometriosis have an immunity dysfunction that enables ectopic endometrial cells to implant and proliferate. Previous studies show that natural killer cells have a pivotal role in the immune control of endometriosis.

METHODS AND FINDINGS

This is a prospective laboratory study conducted in a tertiary-care university hospital between January 2011 and April 2013. We investigated non-pregnant, younger than 42-year-old patients (n= 202) during surgery for benign gynaecological conditions. After complete surgical exploration of the abdominopelvic cavity, 121 women with histologically proven endometriosis and 81 endometriosis-free controls women were enrolled. Patients with endometriosis were classified according to a surgical classification in three different types of endometriosis: superficial peritoneal endometriosis (SUP), ovarian endometrioma (OMA) and deep infiltrating endometriosis (DIE). Peritoneal fluid samples were obtained from all study participants during the surgery in order to detect soluble NKG2D ligands (MICA, MICB and ULBP-2). When samples with undetectable peritoneal fluid levels of MICA, MICB and ULBP-2 were excluded, MICA ratio levels were significantly higher in endometriosis patients than in controls (median, 1.1 pg/mg; range, 0.1-143.5 versus median, 0.6 pg/mg; range, 0.1-3.5; p=0.003). In a similar manner peritoneal fluid MICB levels were also increased in endometriosis-affected patients compared with disease-free women (median, 4.6 pg/mg; range, 1.2-4702 versus median, 3.4 pg/mg; range, 0.7-20.1; p=0.001). According to the surgical classification, peritoneal fluid soluble MICA, MICB and ULBP-2 ratio levels were significantly increased in DIE as compared to controls (p=0.015, p=0.003 and p=0.045 respectively). MICA ratio levels also correlated with dysmenorrhea (r=0.232; p=0.029), total rAFS score (r=0.221; p=0.031) and adhesions rAFS score (r=0.221; p=0.031).

CONCLUSIONS

We demonstrate a significant increase of peritoneal fluid NKG2D ligands in women with endometriosis especially in those cases presenting DIE. This study suggests that NKG2D ligands shedding is a novel pathway in endometriosis complex pathogenesis that impairs NK cell function.

Authors+Show Affiliations

Département Développement, Reproduction et Cancer, Institut Cochin, INSERM, Paris, France; Université Paris Descartes, Sorbone Paris Cité, Faculté de Médecine, Assistance Publique-Hôpitaux de Paris (AP-HP), Groupe Hospitalier Universitaire (GHU) Ouest, Centre Hospitalier Universitaire (CHU) Cochin, Department of Gynecology Obstetrics II and Reproductive Medicine, Paris, France; Institut Clinic of Gynecology, Obstetrics and Neonatology, Hospital Clínic-Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Faculty of Medicine-University of Barcelona, Barcelona, Spain.Département Développement, Reproduction et Cancer, Institut Cochin, INSERM, Paris, France; Université Paris Descartes, Sorbone Paris Cité, Faculté de Médecine, Assistance Publique-Hôpitaux de Paris (AP-HP), Groupe Hospitalier Universitaire (GHU) Ouest, Centre Hospitalier Universitaire (CHU) Cochin, Department of Gynecology Obstetrics II and Reproductive Medicine, Paris, France.Département Développement, Reproduction et Cancer, Institut Cochin, INSERM, Paris, France.Institut Clinic of Gynecology, Obstetrics and Neonatology, Hospital Clínic-Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Faculty of Medicine-University of Barcelona, Barcelona, Spain.Département Développement, Reproduction et Cancer, Institut Cochin, INSERM, Paris, France; Service d'immunologie biologique, Hôpital Cochin, Paris cedex 14, France; DHU Risque et grossesse, Hôpital Cochin, Paris cedex 14, France.Département Développement, Reproduction et Cancer, Institut Cochin, INSERM, Paris, France; Université Paris Descartes, Sorbone Paris Cité, Faculté de Médecine, Assistance Publique-Hôpitaux de Paris (AP-HP), Groupe Hospitalier Universitaire (GHU) Ouest, Centre Hospitalier Universitaire (CHU) Cochin, Department of Gynecology Obstetrics II and Reproductive Medicine, Paris, France; DHU Risque et grossesse, Hôpital Cochin, Paris cedex 14, France.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25775242

Citation

González-Foruria, Iñaki, et al. "Soluble Ligands for the NKG2D Receptor Are Released During Endometriosis and Correlate With Disease Severity." PloS One, vol. 10, no. 3, 2015, pp. e0119961.
González-Foruria I, Santulli P, Chouzenoux S, et al. Soluble ligands for the NKG2D receptor are released during endometriosis and correlate with disease severity. PLoS ONE. 2015;10(3):e0119961.
González-Foruria, I., Santulli, P., Chouzenoux, S., Carmona, F., Batteux, F., & Chapron, C. (2015). Soluble ligands for the NKG2D receptor are released during endometriosis and correlate with disease severity. PloS One, 10(3), pp. e0119961. doi:10.1371/journal.pone.0119961.
González-Foruria I, et al. Soluble Ligands for the NKG2D Receptor Are Released During Endometriosis and Correlate With Disease Severity. PLoS ONE. 2015;10(3):e0119961. PubMed PMID: 25775242.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Soluble ligands for the NKG2D receptor are released during endometriosis and correlate with disease severity. AU - González-Foruria,Iñaki, AU - Santulli,Pietro, AU - Chouzenoux,Sandrine, AU - Carmona,Francisco, AU - Batteux,Frédéric, AU - Chapron,Charles, Y1 - 2015/03/16/ PY - 2014/10/15/received PY - 2015/01/18/accepted PY - 2015/3/17/entrez PY - 2015/3/17/pubmed PY - 2016/1/29/medline SP - e0119961 EP - e0119961 JF - PloS one JO - PLoS ONE VL - 10 IS - 3 N2 - BACKGROUND: Endometriosis is a benign gynaecological disease. Abundant bulk of evidence suggests that patients with endometriosis have an immunity dysfunction that enables ectopic endometrial cells to implant and proliferate. Previous studies show that natural killer cells have a pivotal role in the immune control of endometriosis. METHODS AND FINDINGS: This is a prospective laboratory study conducted in a tertiary-care university hospital between January 2011 and April 2013. We investigated non-pregnant, younger than 42-year-old patients (n= 202) during surgery for benign gynaecological conditions. After complete surgical exploration of the abdominopelvic cavity, 121 women with histologically proven endometriosis and 81 endometriosis-free controls women were enrolled. Patients with endometriosis were classified according to a surgical classification in three different types of endometriosis: superficial peritoneal endometriosis (SUP), ovarian endometrioma (OMA) and deep infiltrating endometriosis (DIE). Peritoneal fluid samples were obtained from all study participants during the surgery in order to detect soluble NKG2D ligands (MICA, MICB and ULBP-2). When samples with undetectable peritoneal fluid levels of MICA, MICB and ULBP-2 were excluded, MICA ratio levels were significantly higher in endometriosis patients than in controls (median, 1.1 pg/mg; range, 0.1-143.5 versus median, 0.6 pg/mg; range, 0.1-3.5; p=0.003). In a similar manner peritoneal fluid MICB levels were also increased in endometriosis-affected patients compared with disease-free women (median, 4.6 pg/mg; range, 1.2-4702 versus median, 3.4 pg/mg; range, 0.7-20.1; p=0.001). According to the surgical classification, peritoneal fluid soluble MICA, MICB and ULBP-2 ratio levels were significantly increased in DIE as compared to controls (p=0.015, p=0.003 and p=0.045 respectively). MICA ratio levels also correlated with dysmenorrhea (r=0.232; p=0.029), total rAFS score (r=0.221; p=0.031) and adhesions rAFS score (r=0.221; p=0.031). CONCLUSIONS: We demonstrate a significant increase of peritoneal fluid NKG2D ligands in women with endometriosis especially in those cases presenting DIE. This study suggests that NKG2D ligands shedding is a novel pathway in endometriosis complex pathogenesis that impairs NK cell function. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/25775242/Soluble_ligands_for_the_NKG2D_receptor_are_released_during_endometriosis_and_correlate_with_disease_severity_ L2 - http://dx.plos.org/10.1371/journal.pone.0119961 DB - PRIME DP - Unbound Medicine ER -