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[Research on UPLC-PDA fingerprint of andrographis paniculata and quantitative determination of 4 major constituents].
Zhongguo Zhong Yao Za Zhi. 2014 Nov; 39(21):4240-5.ZZ

Abstract

Andrographis paniculata from different parts and origins were analyzed by UPLC-PDA fingerprint to provide refererice for related preparation technology. Using the peak of andrographolide as reference, 27 common peaks were identified, and digitized UPLC-PDA fingerprints for 23 batches of andrographis paniculata were established in this research. Principal component analysis (PCA) was carried out after feature extraction. The contents of andrographolide, neoandrographolide, deoxyandrographolide, dehydroandrographolide were determined by external standard method. The Plackett-Burman design combined with pareto chart was used to analyze the factors influencing the robustness of the method. It was found that the medicinal part has a more remarkable influence on the quality of andrographis paniculata than the origin. The contents of the 4 lactones the differ greatly in the different parts of andrographis paniculata, and the pH of the mobile phase is an important factor that influenced the robustness of the method.

Authors

Huang JY
No affiliation info available
Liu XL
No affiliation info available
Zhou SP
No affiliation info available
Tong L
No affiliation info available
Ding L
No affiliation info available

MeSH

AndrographisChromatography, LiquidDiterpenesDrug StabilityGlucosidesPrincipal Component AnalysisTetrahydronaphthalenes

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

25775801

Citation

Huang, Jing-Yi, et al. "[Research On UPLC-PDA Fingerprint of Andrographis Paniculata and Quantitative Determination of 4 Major Constituents]." Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, vol. 39, no. 21, 2014, pp. 4240-5.
Huang JY, Liu XL, Zhou SP, et al. [Research on UPLC-PDA fingerprint of andrographis paniculata and quantitative determination of 4 major constituents]. Zhongguo Zhong Yao Za Zhi. 2014;39(21):4240-5.
Huang, J. Y., Liu, X. L., Zhou, S. P., Tong, L., & Ding, L. (2014). [Research on UPLC-PDA fingerprint of andrographis paniculata and quantitative determination of 4 major constituents]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, 39(21), 4240-5.
Huang JY, et al. [Research On UPLC-PDA Fingerprint of Andrographis Paniculata and Quantitative Determination of 4 Major Constituents]. Zhongguo Zhong Yao Za Zhi. 2014;39(21):4240-5. PubMed PMID: 25775801.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Research on UPLC-PDA fingerprint of andrographis paniculata and quantitative determination of 4 major constituents]. AU - Huang,Jing-Yi, AU - Liu,Xiao-Lin, AU - Zhou,Shui-Ping, AU - Tong,Ling, AU - Ding,Li, PY - 2015/3/18/entrez PY - 2015/3/18/pubmed PY - 2015/5/1/medline SP - 4240 EP - 5 JF - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JO - Zhongguo Zhong Yao Za Zhi VL - 39 IS - 21 N2 - Andrographis paniculata from different parts and origins were analyzed by UPLC-PDA fingerprint to provide refererice for related preparation technology. Using the peak of andrographolide as reference, 27 common peaks were identified, and digitized UPLC-PDA fingerprints for 23 batches of andrographis paniculata were established in this research. Principal component analysis (PCA) was carried out after feature extraction. The contents of andrographolide, neoandrographolide, deoxyandrographolide, dehydroandrographolide were determined by external standard method. The Plackett-Burman design combined with pareto chart was used to analyze the factors influencing the robustness of the method. It was found that the medicinal part has a more remarkable influence on the quality of andrographis paniculata than the origin. The contents of the 4 lactones the differ greatly in the different parts of andrographis paniculata, and the pH of the mobile phase is an important factor that influenced the robustness of the method. SN - 1001-5302 UR - https://www.unboundmedicine.com/medline/citation/25775801/[Research_on_UPLC_PDA_fingerprint_of_andrographis_paniculata_and_quantitative_determination_of_4_major_constituents]_ DB - PRIME DP - Unbound Medicine ER -