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L-tetrahydropalamatine inhibits tumor necrosis factor-α-induced monocyte-endothelial cell adhesion through downregulation of intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 involving suppression of nuclear factor-κ B signaling pathway.
Chin J Integr Med. 2015 May; 21(5):361-8.CJ

Abstract

OBJECTIVE

To investigate whether I-tetrahydropalmatine (I-THP), an alkaloid mainly present in Corydalis family, could ameliorate early vascular inflammatory responses in atherosclerotic processes.

METHODS

Fluorescently labeled monocytes were co-incubated with human umbilical vein endothelial cells (HUVECs), which were pretreated with I-THP and then simulated with tumor necrosis factor (TNF)-α in absence of I-THP to determine if I-THP could reduce thecytokine-induced adhesion of monocytes to HUVECs. Then I-THP were further studied the underlying mechanisms through observing the transcriptional and translational level of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) and the nuclear translocation of nuclear factor (NF)-κ B in HUVECs.

RESULTS

L-THP could block TNF-α-induced adhesion of monocytes to HUVECs and could significantly inhibited the expression of ICAM-1 and VCAM-1 on cell surface by 31% and 36% at 30 μ mol/L. L-THP pretreatment could also markedly reduce transcriptional and translational level of VCAM-1 as well as mildly reduce the total protein and mRNA expression levels of ICAM-1. Furthermore, I-THP attenuated TNF-α-stimulated NF-κ B nuclear translocation.

CONCLUSION

These results provide evidences supporting that I-THP could be a promising compound in the prevention and treatment of the early vascular inflammatory reaction in atherosclerosis by inhibiting monocyte adhesion to vascular endothelial cell through downregulating ICAM-1 and VCAM-1 in vascular endothelial cell based on suppressing NF-κ B.

Authors+Show Affiliations

State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25776841

Citation

Yang, Bin-rui, et al. "L-tetrahydropalamatine Inhibits Tumor Necrosis Factor-α-induced Monocyte-endothelial Cell Adhesion Through Downregulation of Intercellular Adhesion Molecule-1 and Vascular Cell Adhesion Molecule-1 Involving Suppression of Nuclear Factor-κ B Signaling Pathway." Chinese Journal of Integrative Medicine, vol. 21, no. 5, 2015, pp. 361-8.
Yang BR, Yu N, Deng YH, et al. L-tetrahydropalamatine inhibits tumor necrosis factor-α-induced monocyte-endothelial cell adhesion through downregulation of intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 involving suppression of nuclear factor-κ B signaling pathway. Chin J Integr Med. 2015;21(5):361-8.
Yang, B. R., Yu, N., Deng, Y. H., Hoi, P. M., Yang, B., Liu, G. Y., Cong, W. H., & Lee, S. M. (2015). L-tetrahydropalamatine inhibits tumor necrosis factor-α-induced monocyte-endothelial cell adhesion through downregulation of intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 involving suppression of nuclear factor-κ B signaling pathway. Chinese Journal of Integrative Medicine, 21(5), 361-8. https://doi.org/10.1007/s11655-015-2165-7
Yang BR, et al. L-tetrahydropalamatine Inhibits Tumor Necrosis Factor-α-induced Monocyte-endothelial Cell Adhesion Through Downregulation of Intercellular Adhesion Molecule-1 and Vascular Cell Adhesion Molecule-1 Involving Suppression of Nuclear Factor-κ B Signaling Pathway. Chin J Integr Med. 2015;21(5):361-8. PubMed PMID: 25776841.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - L-tetrahydropalamatine inhibits tumor necrosis factor-α-induced monocyte-endothelial cell adhesion through downregulation of intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 involving suppression of nuclear factor-κ B signaling pathway. AU - Yang,Bin-rui, AU - Yu,Nan, AU - Deng,Yan-hui, AU - Hoi,Pui Man, AU - Yang,Bin, AU - Liu,Guang-yu, AU - Cong,Wei-hong, AU - Lee,Simon Ming-yuen, Y1 - 2015/03/17/ PY - 2014/06/18/received PY - 2015/3/18/entrez PY - 2015/3/18/pubmed PY - 2016/2/3/medline SP - 361 EP - 8 JF - Chinese journal of integrative medicine JO - Chin J Integr Med VL - 21 IS - 5 N2 - OBJECTIVE: To investigate whether I-tetrahydropalmatine (I-THP), an alkaloid mainly present in Corydalis family, could ameliorate early vascular inflammatory responses in atherosclerotic processes. METHODS: Fluorescently labeled monocytes were co-incubated with human umbilical vein endothelial cells (HUVECs), which were pretreated with I-THP and then simulated with tumor necrosis factor (TNF)-α in absence of I-THP to determine if I-THP could reduce thecytokine-induced adhesion of monocytes to HUVECs. Then I-THP were further studied the underlying mechanisms through observing the transcriptional and translational level of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) and the nuclear translocation of nuclear factor (NF)-κ B in HUVECs. RESULTS: L-THP could block TNF-α-induced adhesion of monocytes to HUVECs and could significantly inhibited the expression of ICAM-1 and VCAM-1 on cell surface by 31% and 36% at 30 μ mol/L. L-THP pretreatment could also markedly reduce transcriptional and translational level of VCAM-1 as well as mildly reduce the total protein and mRNA expression levels of ICAM-1. Furthermore, I-THP attenuated TNF-α-stimulated NF-κ B nuclear translocation. CONCLUSION: These results provide evidences supporting that I-THP could be a promising compound in the prevention and treatment of the early vascular inflammatory reaction in atherosclerosis by inhibiting monocyte adhesion to vascular endothelial cell through downregulating ICAM-1 and VCAM-1 in vascular endothelial cell based on suppressing NF-κ B. SN - 1672-0415 UR - https://www.unboundmedicine.com/medline/citation/25776841/L_tetrahydropalamatine_inhibits_tumor_necrosis_factor_α_induced_monocyte_endothelial_cell_adhesion_through_downregulation_of_intercellular_adhesion_molecule_1_and_vascular_cell_adhesion_molecule_1_involving_suppression_of_nuclear_factor_κ_B_signaling_pathway_ L2 - https://dx.doi.org/10.1007/s11655-015-2165-7 DB - PRIME DP - Unbound Medicine ER -