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L-tetrahydropalamatine inhibits tumor necrosis factor-α-induced monocyte-endothelial cell adhesion through downregulation of intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 involving suppression of nuclear factor-κ B signaling pathway.
Chin J Integr Med. 2015 May; 21(5):361-8.CJ

Abstract

OBJECTIVE

To investigate whether I-tetrahydropalmatine (I-THP), an alkaloid mainly present in Corydalis family, could ameliorate early vascular inflammatory responses in atherosclerotic processes.

METHODS

Fluorescently labeled monocytes were co-incubated with human umbilical vein endothelial cells (HUVECs), which were pretreated with I-THP and then simulated with tumor necrosis factor (TNF)-α in absence of I-THP to determine if I-THP could reduce thecytokine-induced adhesion of monocytes to HUVECs. Then I-THP were further studied the underlying mechanisms through observing the transcriptional and translational level of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) and the nuclear translocation of nuclear factor (NF)-κ B in HUVECs.

RESULTS

L-THP could block TNF-α-induced adhesion of monocytes to HUVECs and could significantly inhibited the expression of ICAM-1 and VCAM-1 on cell surface by 31% and 36% at 30 μ mol/L. L-THP pretreatment could also markedly reduce transcriptional and translational level of VCAM-1 as well as mildly reduce the total protein and mRNA expression levels of ICAM-1. Furthermore, I-THP attenuated TNF-α-stimulated NF-κ B nuclear translocation.

CONCLUSION

These results provide evidences supporting that I-THP could be a promising compound in the prevention and treatment of the early vascular inflammatory reaction in atherosclerosis by inhibiting monocyte adhesion to vascular endothelial cell through downregulating ICAM-1 and VCAM-1 in vascular endothelial cell based on suppressing NF-κ B.

Links

Publisher Full Text

Authors+Show Affiliations

Yang BR
State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China.
Yu N
No affiliation info available
Deng YH
No affiliation info available
Hoi PM
No affiliation info available
Yang B
No affiliation info available
Liu GY
No affiliation info available
Cong WH
No affiliation info available
Lee SM
No affiliation info available

MeSH

Berberine AlkaloidsCell AdhesionCell NucleusDown-RegulationHuman Umbilical Vein Endothelial CellsHumansIntercellular Adhesion Molecule-1MonocytesNF-kappa BProtein TransportRNA, MessengerSignal TransductionTranscription Factor RelATumor Necrosis Factor-alphaVascular Cell Adhesion Molecule-1

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25776841

Citation

Yang, Bin-rui, et al. "L-tetrahydropalamatine Inhibits Tumor Necrosis Factor-α-induced Monocyte-endothelial Cell Adhesion Through Downregulation of Intercellular Adhesion Molecule-1 and Vascular Cell Adhesion Molecule-1 Involving Suppression of Nuclear Factor-κ B Signaling Pathway." Chinese Journal of Integrative Medicine, vol. 21, no. 5, 2015, pp. 361-8.
Yang BR, Yu N, Deng YH, et al. L-tetrahydropalamatine inhibits tumor necrosis factor-α-induced monocyte-endothelial cell adhesion through downregulation of intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 involving suppression of nuclear factor-κ B signaling pathway. Chin J Integr Med. 2015;21(5):361-8.
Yang, B. R., Yu, N., Deng, Y. H., Hoi, P. M., Yang, B., Liu, G. Y., Cong, W. H., & Lee, S. M. (2015). L-tetrahydropalamatine inhibits tumor necrosis factor-α-induced monocyte-endothelial cell adhesion through downregulation of intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 involving suppression of nuclear factor-κ B signaling pathway. Chinese Journal of Integrative Medicine, 21(5), 361-8. https://doi.org/10.1007/s11655-015-2165-7
Yang BR, et al. L-tetrahydropalamatine Inhibits Tumor Necrosis Factor-α-induced Monocyte-endothelial Cell Adhesion Through Downregulation of Intercellular Adhesion Molecule-1 and Vascular Cell Adhesion Molecule-1 Involving Suppression of Nuclear Factor-κ B Signaling Pathway. Chin J Integr Med. 2015;21(5):361-8. PubMed PMID: 25776841.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - L-tetrahydropalamatine inhibits tumor necrosis factor-α-induced monocyte-endothelial cell adhesion through downregulation of intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 involving suppression of nuclear factor-κ B signaling pathway. AU - Yang,Bin-rui, AU - Yu,Nan, AU - Deng,Yan-hui, AU - Hoi,Pui Man, AU - Yang,Bin, AU - Liu,Guang-yu, AU - Cong,Wei-hong, AU - Lee,Simon Ming-yuen, Y1 - 2015/03/17/ PY - 2014/06/18/received PY - 2015/3/18/entrez PY - 2015/3/18/pubmed PY - 2016/2/3/medline SP - 361 EP - 8 JF - Chinese journal of integrative medicine JO - Chin J Integr Med VL - 21 IS - 5 N2 - OBJECTIVE: To investigate whether I-tetrahydropalmatine (I-THP), an alkaloid mainly present in Corydalis family, could ameliorate early vascular inflammatory responses in atherosclerotic processes. METHODS: Fluorescently labeled monocytes were co-incubated with human umbilical vein endothelial cells (HUVECs), which were pretreated with I-THP and then simulated with tumor necrosis factor (TNF)-α in absence of I-THP to determine if I-THP could reduce thecytokine-induced adhesion of monocytes to HUVECs. Then I-THP were further studied the underlying mechanisms through observing the transcriptional and translational level of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) and the nuclear translocation of nuclear factor (NF)-κ B in HUVECs. RESULTS: L-THP could block TNF-α-induced adhesion of monocytes to HUVECs and could significantly inhibited the expression of ICAM-1 and VCAM-1 on cell surface by 31% and 36% at 30 μ mol/L. L-THP pretreatment could also markedly reduce transcriptional and translational level of VCAM-1 as well as mildly reduce the total protein and mRNA expression levels of ICAM-1. Furthermore, I-THP attenuated TNF-α-stimulated NF-κ B nuclear translocation. CONCLUSION: These results provide evidences supporting that I-THP could be a promising compound in the prevention and treatment of the early vascular inflammatory reaction in atherosclerosis by inhibiting monocyte adhesion to vascular endothelial cell through downregulating ICAM-1 and VCAM-1 in vascular endothelial cell based on suppressing NF-κ B. SN - 1672-0415 UR - https://www.unboundmedicine.com/medline/citation/25776841/L_tetrahydropalamatine_inhibits_tumor_necrosis_factor_α_induced_monocyte_endothelial_cell_adhesion_through_downregulation_of_intercellular_adhesion_molecule_1_and_vascular_cell_adhesion_molecule_1_involving_suppression_of_nuclear_factor_κ_B_signaling_pathway_ L2 - https://dx.doi.org/10.1007/s11655-015-2165-7 DB - PRIME DP - Unbound Medicine ER -
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