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Overexpressing CYP71Z2 enhances resistance to bacterial blight by suppressing auxin biosynthesis in rice.
PLoS One. 2015; 10(3):e0119867.Plos

Abstract

BACKGROUND

The hormone auxin plays an important role not only in the growth and development of rice, but also in its defense responses. We've previously shown that the P450 gene CYP71Z2 enhances disease resistance to pathogens through regulation of phytoalexin biosynthesis in rice, though it remains unclear if auxin is involved in this process or not.

METHODOLOGY AND PRINCIPAL FINDINGS

The expression of CYP71Z2 was induced by Xanthomonas oryzae pv. oryzae (Xoo) inoculation was analyzed by qRT-PCR, with GUS histochemical staining showing that CYP71Z2 expression was limited to roots, blades and nodes. Overexpression of CYP71Z2 in rice durably and stably increased resistance to Xoo, though no significant difference in disease resistance was detected between CYP71Z2-RNA interference (RNAi) rice and wild-type. Moreover, IAA concentration was determined using the HPLC/electrospray ionization/tandem mass spectrometry system. The accumulation of IAA was significantly reduced in CYP71Z2-overexpressing rice regardless of whether plants were inoculated or not, whereas it was unaffected in CYP71Z2-RNAi rice. Furthermore, the expression of genes related to IAA, expansin and SA/JA signaling pathways was suppressed in CYP71Z2-overexpressing rice with or without inoculation.

CONCLUSIONS AND SIGNIFICANCE

These results suggest that CYP71Z2-mediated resistance to Xoo may be via suppression of IAA signaling in rice. Our studies also provide comprehensive insight into molecular mechanism of resistance to Xoo mediated by IAA in rice. Moreover, an available approach for understanding the P450 gene functions in interaction between rice and pathogens has been provided.

Authors+Show Affiliations

Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Nanjing Branch of Chinese National Center for Rice Improvement/Jiangsu High Quality Rice R&D Center, Nanjing 210014, China.Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Nanjing Branch of Chinese National Center for Rice Improvement/Jiangsu High Quality Rice R&D Center, Nanjing 210014, China.Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Nanjing Branch of Chinese National Center for Rice Improvement/Jiangsu High Quality Rice R&D Center, Nanjing 210014, China.Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Nanjing Branch of Chinese National Center for Rice Improvement/Jiangsu High Quality Rice R&D Center, Nanjing 210014, China.Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Nanjing Branch of Chinese National Center for Rice Improvement/Jiangsu High Quality Rice R&D Center, Nanjing 210014, China.Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Nanjing Branch of Chinese National Center for Rice Improvement/Jiangsu High Quality Rice R&D Center, Nanjing 210014, China.Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Nanjing Branch of Chinese National Center for Rice Improvement/Jiangsu High Quality Rice R&D Center, Nanjing 210014, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25786239

Citation

Li, Wenqi, et al. "Overexpressing CYP71Z2 Enhances Resistance to Bacterial Blight By Suppressing Auxin Biosynthesis in Rice." PloS One, vol. 10, no. 3, 2015, pp. e0119867.
Li W, Wang F, Wang J, et al. Overexpressing CYP71Z2 enhances resistance to bacterial blight by suppressing auxin biosynthesis in rice. PLoS ONE. 2015;10(3):e0119867.
Li, W., Wang, F., Wang, J., Fan, F., Zhu, J., Yang, J., Liu, F., & Zhong, W. (2015). Overexpressing CYP71Z2 enhances resistance to bacterial blight by suppressing auxin biosynthesis in rice. PloS One, 10(3), e0119867. https://doi.org/10.1371/journal.pone.0119867
Li W, et al. Overexpressing CYP71Z2 Enhances Resistance to Bacterial Blight By Suppressing Auxin Biosynthesis in Rice. PLoS ONE. 2015;10(3):e0119867. PubMed PMID: 25786239.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Overexpressing CYP71Z2 enhances resistance to bacterial blight by suppressing auxin biosynthesis in rice. AU - Li,Wenqi, AU - Wang,Fangquan, AU - Wang,Jun, AU - Fan,Fangjun, AU - Zhu,Jinyan, AU - Yang,Jie, AU - Liu,Fengquan, AU - Zhong,Weigong, Y1 - 2015/03/18/ PY - 2014/11/13/received PY - 2015/01/16/accepted PY - 2015/3/19/entrez PY - 2015/3/19/pubmed PY - 2016/2/9/medline SP - e0119867 EP - e0119867 JF - PloS one JO - PLoS ONE VL - 10 IS - 3 N2 - BACKGROUND: The hormone auxin plays an important role not only in the growth and development of rice, but also in its defense responses. We've previously shown that the P450 gene CYP71Z2 enhances disease resistance to pathogens through regulation of phytoalexin biosynthesis in rice, though it remains unclear if auxin is involved in this process or not. METHODOLOGY AND PRINCIPAL FINDINGS: The expression of CYP71Z2 was induced by Xanthomonas oryzae pv. oryzae (Xoo) inoculation was analyzed by qRT-PCR, with GUS histochemical staining showing that CYP71Z2 expression was limited to roots, blades and nodes. Overexpression of CYP71Z2 in rice durably and stably increased resistance to Xoo, though no significant difference in disease resistance was detected between CYP71Z2-RNA interference (RNAi) rice and wild-type. Moreover, IAA concentration was determined using the HPLC/electrospray ionization/tandem mass spectrometry system. The accumulation of IAA was significantly reduced in CYP71Z2-overexpressing rice regardless of whether plants were inoculated or not, whereas it was unaffected in CYP71Z2-RNAi rice. Furthermore, the expression of genes related to IAA, expansin and SA/JA signaling pathways was suppressed in CYP71Z2-overexpressing rice with or without inoculation. CONCLUSIONS AND SIGNIFICANCE: These results suggest that CYP71Z2-mediated resistance to Xoo may be via suppression of IAA signaling in rice. Our studies also provide comprehensive insight into molecular mechanism of resistance to Xoo mediated by IAA in rice. Moreover, an available approach for understanding the P450 gene functions in interaction between rice and pathogens has been provided. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/25786239/Overexpressing_CYP71Z2_enhances_resistance_to_bacterial_blight_by_suppressing_auxin_biosynthesis_in_rice_ L2 - http://dx.plos.org/10.1371/journal.pone.0119867 DB - PRIME DP - Unbound Medicine ER -