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Early osmotic, antioxidant, ionic, and redox responses to salinity in leaves and roots of Indian mustard (Brassica juncea L.).
Protoplasma. 2016 Jan; 253(1):101-10.P

Abstract

Salt-stress-induced alterations in osmotic, ionic, and redox responses were studied in the early period of treatment (30 min to 5 days) in seedlings of Brassica juncea L. Roots and shoots under mild (50 mM) and severe (250 mM) NaCl stress were analyzed for growth, oxidative stress, osmolyte accumulation, antioxidant defense, and redox state. Growth reduction was less pronounced in the early time period of salt stress while oxidative damage increased linearly and in a sustained manner under severe stress up to 6 h. An early and transient reactive oxygen species (ROS) burst, as evidenced by superoxide and hydrogen peroxide level was observed, followed by activation of enzymatic antioxidant system (GPX, SOD, CAT, and GR) in both root and shoot. The enzymatic activity was not affected much under mild stress particularly at early phase; however, severe stress induced a significant increase in the activity of antioxidant enzymes. Root ascorbate was progressively accumulated, and its redox state maintained in the early time phase of treatment under mild stress while increase in root and shoot glutathione content was recorded under mild stress at 5 days when the active ascorbate pool decreased. While early period of salt stress showed significant Na(+) accumulation over control, plants subjected to mild stress measured less Na(+) accumulation up to 5 days compared to severely stressed plants. The results showed an early induction of differential responses to salt stress in roots and shoots of Brassica which include growth limitations, reduced relative water content, increased osmolytes, redox state, and antioxidant system, and a significant Na(+) increase. The results also indicate that roots and shoots may have distinct mechanisms of responses to salt stress.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Ranjit SL
Plant Stress physiology and biotechnology Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai, 400085, India.
Manish P
Plant Stress physiology and biotechnology Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai, 400085, India.
Penna S
Plant Stress physiology and biotechnology Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai, 400085, India. prasanna@barc.gov.in.

MeSH

AntioxidantsBiomassHydrogen PeroxideIonsMustard PlantOsmosisOxidation-ReductionPlant LeavesPlant RootsPlant ShootsPrincipal Component AnalysisSalinitySeedlingsSodium ChlorideStress, PhysiologicalWater

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25786350

Citation

Ranjit, Singh Laxmi, et al. "Early Osmotic, Antioxidant, Ionic, and Redox Responses to Salinity in Leaves and Roots of Indian Mustard (Brassica Juncea L.)." Protoplasma, vol. 253, no. 1, 2016, pp. 101-10.
Ranjit SL, Manish P, Penna S. Early osmotic, antioxidant, ionic, and redox responses to salinity in leaves and roots of Indian mustard (Brassica juncea L.). Protoplasma. 2016;253(1):101-10.
Ranjit, S. L., Manish, P., & Penna, S. (2016). Early osmotic, antioxidant, ionic, and redox responses to salinity in leaves and roots of Indian mustard (Brassica juncea L.). Protoplasma, 253(1), 101-10. https://doi.org/10.1007/s00709-015-0792-7
Ranjit SL, Manish P, Penna S. Early Osmotic, Antioxidant, Ionic, and Redox Responses to Salinity in Leaves and Roots of Indian Mustard (Brassica Juncea L.). Protoplasma. 2016;253(1):101-10. PubMed PMID: 25786350.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Early osmotic, antioxidant, ionic, and redox responses to salinity in leaves and roots of Indian mustard (Brassica juncea L.). AU - Ranjit,Singh Laxmi, AU - Manish,Pandey, AU - Penna,Suprasanna, Y1 - 2015/03/19/ PY - 2014/07/27/received PY - 2015/03/02/accepted PY - 2015/3/20/entrez PY - 2015/3/20/pubmed PY - 2016/10/11/medline KW - Antioxidant enzymes KW - Brassica KW - Early events KW - H2O2 KW - Roots and shoots KW - Salt stress KW - Sodium-potassium SP - 101 EP - 10 JF - Protoplasma JO - Protoplasma VL - 253 IS - 1 N2 - Salt-stress-induced alterations in osmotic, ionic, and redox responses were studied in the early period of treatment (30 min to 5 days) in seedlings of Brassica juncea L. Roots and shoots under mild (50 mM) and severe (250 mM) NaCl stress were analyzed for growth, oxidative stress, osmolyte accumulation, antioxidant defense, and redox state. Growth reduction was less pronounced in the early time period of salt stress while oxidative damage increased linearly and in a sustained manner under severe stress up to 6 h. An early and transient reactive oxygen species (ROS) burst, as evidenced by superoxide and hydrogen peroxide level was observed, followed by activation of enzymatic antioxidant system (GPX, SOD, CAT, and GR) in both root and shoot. The enzymatic activity was not affected much under mild stress particularly at early phase; however, severe stress induced a significant increase in the activity of antioxidant enzymes. Root ascorbate was progressively accumulated, and its redox state maintained in the early time phase of treatment under mild stress while increase in root and shoot glutathione content was recorded under mild stress at 5 days when the active ascorbate pool decreased. While early period of salt stress showed significant Na(+) accumulation over control, plants subjected to mild stress measured less Na(+) accumulation up to 5 days compared to severely stressed plants. The results showed an early induction of differential responses to salt stress in roots and shoots of Brassica which include growth limitations, reduced relative water content, increased osmolytes, redox state, and antioxidant system, and a significant Na(+) increase. The results also indicate that roots and shoots may have distinct mechanisms of responses to salt stress. SN - 1615-6102 UR - https://www.unboundmedicine.com/medline/citation/25786350/Early_osmotic_antioxidant_ionic_and_redox_responses_to_salinity_in_leaves_and_roots_of_Indian_mustard__Brassica_juncea_L___ DB - PRIME DP - Unbound Medicine ER -