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Control of ColE1 plasmid replication: initial interaction of RNA I and the primer transcript is reversible.
Cell. 1985 Mar; 40(3):527-35.Cell

Abstract

Replication of ColE1-type plasmids is known to be regulated by a plasmid-specific RNA (RNA I), whose binding to the transcript (RNA II) from the primer promoter results in inhibition of formation of the primer for DNA replication. In this paper, it is shown that binding of RNA I to the homologous RNA II is inhibited by an RNA I specified by a plasmid of different compatibility. The inhibition is caused by the reversible interaction of RNA II with the heterologous RNA I, which competes with reversible interaction of the two homologous RNAs at a step preceding their stable binding. As predicted from these results, the copy numbers of both ColE1 and RSF1030 are increased when both plasmids are present in the same cell. The Rom protein enhances the reversible interaction.

Authors

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Pub Type(s)

Journal Article

Language

eng

PubMed ID

2578886

Citation

Tomizawa, J. "Control of ColE1 Plasmid Replication: Initial Interaction of RNA I and the Primer Transcript Is Reversible." Cell, vol. 40, no. 3, 1985, pp. 527-35.
Tomizawa J. Control of ColE1 plasmid replication: initial interaction of RNA I and the primer transcript is reversible. Cell. 1985;40(3):527-35.
Tomizawa, J. (1985). Control of ColE1 plasmid replication: initial interaction of RNA I and the primer transcript is reversible. Cell, 40(3), 527-35.
Tomizawa J. Control of ColE1 Plasmid Replication: Initial Interaction of RNA I and the Primer Transcript Is Reversible. Cell. 1985;40(3):527-35. PubMed PMID: 2578886.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Control of ColE1 plasmid replication: initial interaction of RNA I and the primer transcript is reversible. A1 - Tomizawa,J, PY - 1985/3/1/pubmed PY - 1985/3/1/medline PY - 1985/3/1/entrez SP - 527 EP - 35 JF - Cell JO - Cell VL - 40 IS - 3 N2 - Replication of ColE1-type plasmids is known to be regulated by a plasmid-specific RNA (RNA I), whose binding to the transcript (RNA II) from the primer promoter results in inhibition of formation of the primer for DNA replication. In this paper, it is shown that binding of RNA I to the homologous RNA II is inhibited by an RNA I specified by a plasmid of different compatibility. The inhibition is caused by the reversible interaction of RNA II with the heterologous RNA I, which competes with reversible interaction of the two homologous RNAs at a step preceding their stable binding. As predicted from these results, the copy numbers of both ColE1 and RSF1030 are increased when both plasmids are present in the same cell. The Rom protein enhances the reversible interaction. SN - 0092-8674 UR - https://www.unboundmedicine.com/medline/citation/2578886/Control_of_ColE1_plasmid_replication:_initial_interaction_of_RNA_I_and_the_primer_transcript_is_reversible_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0092-8674(85)90201-6 DB - PRIME DP - Unbound Medicine ER -