Tags

Type your tag names separated by a space and hit enter

Structural features, IgE binding and preliminary clinical findings of the 7kDa Lipid Transfer Protein from tomato seeds.
Mol Immunol. 2015 Aug; 66(2):154-63.MI

Abstract

Allergic reactions caused by 9kDa Lipid Transfer Proteins (9k-LTP), such as Pru p 3, have been widely investigated, whereas a possible contribution of components of 7kDa LTP (7k-LTP) sub-family in triggering allergic symptoms has been overlooked so far. With the aim to investigate the contribution of 7k-LTPs to the food allergies, we have identified, isolated and characterised a tomato seed 7k-LTP (Sola l 7k-LTP). The protein was purified by chromatographic separations, identified by direct protein sequencing and mass spectrometry and a molecular model was built. Functional evaluation of the allergen has been performed by skin testing. Sola l 7k-LTP consists of 68 amino acids producing a molecular mass of 7045Da and displays 41% sequence identity with Pru p 3, the allergenic 9k-LTP from peach. IgE antibodies specifically recognising Sola l 7k-LTP were found within the population claiming tomato ingestion-related symptoms, but also in subjects tolerant on tomato exposure. A few subjects were mono-sensitised to Sola l 7k-LTP, which is biologically active as shown by the positive skin test. In line with the immunological results, the molecular model shows structural similarities between the IgE binding regions of the two sub-families. Therefore, Sola l 7k-LTP shares some structural and immunological features with Pru p 3, but it also displays individual features that could be responsible for mono-specific IgE binding. In conclusion, Sola l 7k-LTP is a new identified allergenic LTP, the description of which may contribute to the improvement of allergy diagnosis and to the formulation of a safe and personalised diet. In addition, to avoid current confusing classifications, a new nomenclature policy for LTP sub-families is proposed in this paper. We now suggest that 7-kDa LTP (so far named LTP2) be renamed 7k-LTP and 9-kDa LTP (so far named LTP1) be renamed 9k-LTP.

Authors+Show Affiliations

Institute of Biosciences and BioResources, CNR, Via Pietro Castellino 111, I-80131 Naples, Italy.Center for Molecular Allergology, IDI-IRCCS, Rome, Italy; Associated Centres for Molecular Allergology, Rome and Latium, Italy.Center for Molecular Allergology, IDI-IRCCS, Rome, Italy; Associated Centres for Molecular Allergology, Rome and Latium, Italy.Center for Molecular Allergology, IDI-IRCCS, Rome, Italy; Associated Centres for Molecular Allergology, Rome and Latium, Italy.Center for Molecular Allergology, IDI-IRCCS, Rome, Italy; Associated Centres for Molecular Allergology, Rome and Latium, Italy.Institute of Biosciences and BioResources, CNR, Via Pietro Castellino 111, I-80131 Naples, Italy.Institute of Biosciences and BioResources, CNR, Via Pietro Castellino 111, I-80131 Naples, Italy.Institute of Biosciences and BioResources, CNR, Via Pietro Castellino 111, I-80131 Naples, Italy.Institute of Biosciences and BioResources, CNR, Via Pietro Castellino 111, I-80131 Naples, Italy.Center for Molecular Allergology, IDI-IRCCS, Rome, Italy; Associated Centres for Molecular Allergology, Rome and Latium, Italy. Electronic address: adriano.mari@caam-allergy.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25791776

Citation

Giangrieco, Ivana, et al. "Structural Features, IgE Binding and Preliminary Clinical Findings of the 7kDa Lipid Transfer Protein From Tomato Seeds." Molecular Immunology, vol. 66, no. 2, 2015, pp. 154-63.
Giangrieco I, Alessandri C, Rafaiani C, et al. Structural features, IgE binding and preliminary clinical findings of the 7kDa Lipid Transfer Protein from tomato seeds. Mol Immunol. 2015;66(2):154-63.
Giangrieco, I., Alessandri, C., Rafaiani, C., Santoro, M., Zuzzi, S., Tuppo, L., Tamburrini, M., D'Avino, R., Ciardiello, M. A., & Mari, A. (2015). Structural features, IgE binding and preliminary clinical findings of the 7kDa Lipid Transfer Protein from tomato seeds. Molecular Immunology, 66(2), 154-63. https://doi.org/10.1016/j.molimm.2015.02.025
Giangrieco I, et al. Structural Features, IgE Binding and Preliminary Clinical Findings of the 7kDa Lipid Transfer Protein From Tomato Seeds. Mol Immunol. 2015;66(2):154-63. PubMed PMID: 25791776.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Structural features, IgE binding and preliminary clinical findings of the 7kDa Lipid Transfer Protein from tomato seeds. AU - Giangrieco,Ivana, AU - Alessandri,Claudia, AU - Rafaiani,Chiara, AU - Santoro,Mario, AU - Zuzzi,Sara, AU - Tuppo,Lisa, AU - Tamburrini,Maurizio, AU - D'Avino,Rossana, AU - Ciardiello,Maria Antonietta, AU - Mari,Adriano, Y1 - 2015/03/16/ PY - 2015/01/20/received PY - 2015/02/12/revised PY - 2015/02/19/accepted PY - 2015/3/21/entrez PY - 2015/3/21/pubmed PY - 2015/8/12/medline KW - 7kDa LTP KW - Food allergy KW - IgE reactivity KW - Molecular model KW - Solanum lycopersicum KW - Tomato seeds SP - 154 EP - 63 JF - Molecular immunology JO - Mol Immunol VL - 66 IS - 2 N2 - Allergic reactions caused by 9kDa Lipid Transfer Proteins (9k-LTP), such as Pru p 3, have been widely investigated, whereas a possible contribution of components of 7kDa LTP (7k-LTP) sub-family in triggering allergic symptoms has been overlooked so far. With the aim to investigate the contribution of 7k-LTPs to the food allergies, we have identified, isolated and characterised a tomato seed 7k-LTP (Sola l 7k-LTP). The protein was purified by chromatographic separations, identified by direct protein sequencing and mass spectrometry and a molecular model was built. Functional evaluation of the allergen has been performed by skin testing. Sola l 7k-LTP consists of 68 amino acids producing a molecular mass of 7045Da and displays 41% sequence identity with Pru p 3, the allergenic 9k-LTP from peach. IgE antibodies specifically recognising Sola l 7k-LTP were found within the population claiming tomato ingestion-related symptoms, but also in subjects tolerant on tomato exposure. A few subjects were mono-sensitised to Sola l 7k-LTP, which is biologically active as shown by the positive skin test. In line with the immunological results, the molecular model shows structural similarities between the IgE binding regions of the two sub-families. Therefore, Sola l 7k-LTP shares some structural and immunological features with Pru p 3, but it also displays individual features that could be responsible for mono-specific IgE binding. In conclusion, Sola l 7k-LTP is a new identified allergenic LTP, the description of which may contribute to the improvement of allergy diagnosis and to the formulation of a safe and personalised diet. In addition, to avoid current confusing classifications, a new nomenclature policy for LTP sub-families is proposed in this paper. We now suggest that 7-kDa LTP (so far named LTP2) be renamed 7k-LTP and 9-kDa LTP (so far named LTP1) be renamed 9k-LTP. SN - 1872-9142 UR - https://www.unboundmedicine.com/medline/citation/25791776/Structural_features_IgE_binding_and_preliminary_clinical_findings_of_the_7kDa_Lipid_Transfer_Protein_from_tomato_seeds_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0161-5890(15)00074-7 DB - PRIME DP - Unbound Medicine ER -