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Helicteres sacarolha A. St.- Hil. et al.: gastroprotective and possible mechanism of actions in experimental animals.
J Ethnopharmacol. 2015 May 26; 166:176-84.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Helicteres sacarolha A. St.- Hil. et al. popularly known in Brazil as 'semente-de-macaco', is widely employed in the popular medicine in many of parts of Brazil in the alleviation of symptoms of ailments such as peptic ulcer and inflammation. Up to the present, there is no study addressing the gastroprotective activity of the hydroethanolic extract of H. sacarolha and its possible mechanism of actions.

MATERIALS AND METHODS

The hydroethanolic (70%) extract of H. sacarolha (HEHs) was obtained by maceration. The gastroprotective activity was assessed using gastric ulcer models induced by acidified ethanol, piroxicam, and water restraint stress in mice and rats at doses of 20, 50 and 250mg/kg p.o. Mechanistic studies involved the antisecretory assay evaluated with pylorus ligation in rats and pre-treatments with appropriate antagonists/inhibitors such as yohimbine, glibenclamide, indomethacin and l-NAME, effect on catalase and myeloperoxidase activities and gastric mucus determination using acidified ethanol- induced ulcer in mice.

RESULTS

HEHs at all doses tested demonstrated potent gastroprotective activities in the acute ulcer models. The gastroprotective activity of HEHs was attenuated by pre-treatments with yohimbine, glibenclamide, indomethacin and l-NAME. HEHs effectively reduced basal gastric juice production without any effect on the free and total acidity. The gastroprotective action of HEHs involved increasing the antioxidant enzyme catalase and mucus secretion and inhibition of neutrophyl infiltration as reflected by the reduction in the myeloperoxidase activity.

CONCLUSION

The results of this study gave a scientific support for the popular use of the leaves of H. sacarolha in the treatment of gastric ulcers and that it has a multi-targeted action.

Authors+Show Affiliations

Postgraduate Studies in Health Sciences, Faculty of Medicine, Federal University of Mato Grosso (UFMT), 78060-900 Cuiabá, MT, Brazil.Postgraduate Studies in Health Sciences, Faculty of Medicine, Federal University of Mato Grosso (UFMT), 78060-900 Cuiabá, MT, Brazil; Department of Basic Health Sciences, Faculty of Medicine, Federal University of Mato Grosso (UFMT), 78060-900 Cuiabá, MT, Brazil.Postgraduate Studies in Health Sciences, Faculty of Medicine, Federal University of Mato Grosso (UFMT), 78060-900 Cuiabá, MT, Brazil; Department of Basic Health Sciences, Faculty of Medicine, Federal University of Mato Grosso (UFMT), 78060-900 Cuiabá, MT, Brazil. Electronic address: taba@terra.com.br.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25792014

Citation

Balogun, Sikiru Olaitan, et al. "Helicteres Sacarolha A. St.- Hil. Et Al.: Gastroprotective and Possible Mechanism of Actions in Experimental Animals." Journal of Ethnopharmacology, vol. 166, 2015, pp. 176-84.
Balogun SO, Damazo AS, de Oliveira Martins DT. Helicteres sacarolha A. St.- Hil. et al.: gastroprotective and possible mechanism of actions in experimental animals. J Ethnopharmacol. 2015;166:176-84.
Balogun, S. O., Damazo, A. S., & de Oliveira Martins, D. T. (2015). Helicteres sacarolha A. St.- Hil. et al.: gastroprotective and possible mechanism of actions in experimental animals. Journal of Ethnopharmacology, 166, 176-84. https://doi.org/10.1016/j.jep.2015.03.021
Balogun SO, Damazo AS, de Oliveira Martins DT. Helicteres Sacarolha A. St.- Hil. Et Al.: Gastroprotective and Possible Mechanism of Actions in Experimental Animals. J Ethnopharmacol. 2015 May 26;166:176-84. PubMed PMID: 25792014.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Helicteres sacarolha A. St.- Hil. et al.: gastroprotective and possible mechanism of actions in experimental animals. AU - Balogun,Sikiru Olaitan, AU - Damazo,Amilcar Sabino, AU - de Oliveira Martins,Domingos Tabajara, Y1 - 2015/03/16/ PY - 2014/12/14/received PY - 2015/02/10/revised PY - 2015/03/08/accepted PY - 2015/3/21/entrez PY - 2015/3/21/pubmed PY - 2016/1/8/medline KW - 3,3′,5,5′-tetramethylbenzidine (PubChem CID: 41206) KW - Antioxidant KW - Carbenoxolone (PubChem CID: 6419769) KW - Gastric ulcer KW - Gastroprotection KW - Glibenclamide (PubChem CID: 3488) KW - Helicteres sacarolha KW - Indomethacin (PubChem CID: 3715) KW - Malvaceae KW - Mechanism of action KW - N-ω-Nitro-l-arginine methyl ester hydrochloride (PubChem CID: 135193) KW - Omeprazole (PubChem CID: 4594) KW - Piroxicam (PubChem CID: 54676228) KW - Ranitidine (PubChem CID: 9896548) KW - Yohimbine (PubChem CID: 6169) SP - 176 EP - 84 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 166 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Helicteres sacarolha A. St.- Hil. et al. popularly known in Brazil as 'semente-de-macaco', is widely employed in the popular medicine in many of parts of Brazil in the alleviation of symptoms of ailments such as peptic ulcer and inflammation. Up to the present, there is no study addressing the gastroprotective activity of the hydroethanolic extract of H. sacarolha and its possible mechanism of actions. MATERIALS AND METHODS: The hydroethanolic (70%) extract of H. sacarolha (HEHs) was obtained by maceration. The gastroprotective activity was assessed using gastric ulcer models induced by acidified ethanol, piroxicam, and water restraint stress in mice and rats at doses of 20, 50 and 250mg/kg p.o. Mechanistic studies involved the antisecretory assay evaluated with pylorus ligation in rats and pre-treatments with appropriate antagonists/inhibitors such as yohimbine, glibenclamide, indomethacin and l-NAME, effect on catalase and myeloperoxidase activities and gastric mucus determination using acidified ethanol- induced ulcer in mice. RESULTS: HEHs at all doses tested demonstrated potent gastroprotective activities in the acute ulcer models. The gastroprotective activity of HEHs was attenuated by pre-treatments with yohimbine, glibenclamide, indomethacin and l-NAME. HEHs effectively reduced basal gastric juice production without any effect on the free and total acidity. The gastroprotective action of HEHs involved increasing the antioxidant enzyme catalase and mucus secretion and inhibition of neutrophyl infiltration as reflected by the reduction in the myeloperoxidase activity. CONCLUSION: The results of this study gave a scientific support for the popular use of the leaves of H. sacarolha in the treatment of gastric ulcers and that it has a multi-targeted action. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/25792014/Helicteres_sacarolha_A__St___Hil__et_al_:_gastroprotective_and_possible_mechanism_of_actions_in_experimental_animals_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(15)00165-8 DB - PRIME DP - Unbound Medicine ER -