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Identification of proanthocyanidins from litchi (Litchi chinensis Sonn.) pulp by LC-ESI-Q-TOF-MS and their antioxidant activity.
PLoS One. 2015; 10(3):e0120480.Plos

Abstract

Content of total proanthocyanidins as well as total phenolics, flavonoids, antioxidant activities were evaluated for litchi (Litchi chinensis Sonn.) pulp of 32 cultivars. One cultivar, Hemaoli, showed the highest total proanthocyanidins and total phenolics, and DPPH or ABTS radical scavenging activities. ESI-MS and NMR analysis of the Hemaoli pulp crude extracts (HPCE) showed that procyandins composed of (epi)catechin unites with degree of polymerization (DP) of 2-6 were dominant proanthocyanidins in HPCE. After the HPCE was fractionated by a Sephadex LH-20 column, 32 procyanidins were identified by LC-ESI-Q-TOF-MS in litchi pulp for the first time. Quantification of individual procyanidin in HPCE indicated that epicatechin, procyanidin B2, procyanidin C1 and A-type procyanidin trimer were the main procyanidins. The radical scavenging activities of different fractions of HPCE as well as six procyanidins standards were evaluated by both DPPH and ABTS assays. HPCE fractions showed similar antioxidant activities with those of Vc and six individual procyanidins, the IC50 of which ranged from 1.88 ± 0.01 to 2.82 ± 0.10 μg/ml for DPPH assay, and from 1.52 ± 0.17 to 2.71 ± 0.15 μg/ml for ABTS assay. Such results indicate that litchi cultivars rich in proanthocyanidins are good resources of dietary antioxidants and have the potential to contribute to human health.

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Authors+Show Affiliations

Lv Q
Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou, PR China.
Luo F
Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou, PR China.
Zhao X
Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou, PR China.
Liu Y
College of Life Sciences, Zhejiang University, Zijingang Campus, Hangzhou, PR China.
Hu G
College of Horticulture, South China Agricultural University, Guangzhou, Guangdong, China.
Sun C
Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou, PR China.
Li X
Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou, PR China.
Chen K
Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou, PR China.

MeSH

Free Radical ScavengersLitchiMass SpectrometryPlant ExtractsProanthocyanidins

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25793378

Citation

Lv, Qiang, et al. "Identification of Proanthocyanidins From Litchi (Litchi Chinensis Sonn.) Pulp By LC-ESI-Q-TOF-MS and Their Antioxidant Activity." PloS One, vol. 10, no. 3, 2015, pp. e0120480.
Lv Q, Luo F, Zhao X, et al. Identification of proanthocyanidins from litchi (Litchi chinensis Sonn.) pulp by LC-ESI-Q-TOF-MS and their antioxidant activity. PLoS One. 2015;10(3):e0120480.
Lv, Q., Luo, F., Zhao, X., Liu, Y., Hu, G., Sun, C., Li, X., & Chen, K. (2015). Identification of proanthocyanidins from litchi (Litchi chinensis Sonn.) pulp by LC-ESI-Q-TOF-MS and their antioxidant activity. PloS One, 10(3), e0120480. https://doi.org/10.1371/journal.pone.0120480
Lv Q, et al. Identification of Proanthocyanidins From Litchi (Litchi Chinensis Sonn.) Pulp By LC-ESI-Q-TOF-MS and Their Antioxidant Activity. PLoS One. 2015;10(3):e0120480. PubMed PMID: 25793378.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of proanthocyanidins from litchi (Litchi chinensis Sonn.) pulp by LC-ESI-Q-TOF-MS and their antioxidant activity. AU - Lv,Qiang, AU - Luo,Fenglei, AU - Zhao,Xiaoyong, AU - Liu,Yu, AU - Hu,Guibing, AU - Sun,Chongde, AU - Li,Xian, AU - Chen,Kunsong, Y1 - 2015/03/20/ PY - 2014/08/15/received PY - 2015/01/22/accepted PY - 2015/3/21/entrez PY - 2015/3/21/pubmed PY - 2016/2/10/medline SP - e0120480 EP - e0120480 JF - PloS one JO - PLoS One VL - 10 IS - 3 N2 - Content of total proanthocyanidins as well as total phenolics, flavonoids, antioxidant activities were evaluated for litchi (Litchi chinensis Sonn.) pulp of 32 cultivars. One cultivar, Hemaoli, showed the highest total proanthocyanidins and total phenolics, and DPPH or ABTS radical scavenging activities. ESI-MS and NMR analysis of the Hemaoli pulp crude extracts (HPCE) showed that procyandins composed of (epi)catechin unites with degree of polymerization (DP) of 2-6 were dominant proanthocyanidins in HPCE. After the HPCE was fractionated by a Sephadex LH-20 column, 32 procyanidins were identified by LC-ESI-Q-TOF-MS in litchi pulp for the first time. Quantification of individual procyanidin in HPCE indicated that epicatechin, procyanidin B2, procyanidin C1 and A-type procyanidin trimer were the main procyanidins. The radical scavenging activities of different fractions of HPCE as well as six procyanidins standards were evaluated by both DPPH and ABTS assays. HPCE fractions showed similar antioxidant activities with those of Vc and six individual procyanidins, the IC50 of which ranged from 1.88 ± 0.01 to 2.82 ± 0.10 μg/ml for DPPH assay, and from 1.52 ± 0.17 to 2.71 ± 0.15 μg/ml for ABTS assay. Such results indicate that litchi cultivars rich in proanthocyanidins are good resources of dietary antioxidants and have the potential to contribute to human health. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/25793378/Identification_of_proanthocyanidins_from_litchi__Litchi_chinensis_Sonn___pulp_by_LC_ESI_Q_TOF_MS_and_their_antioxidant_activity_ DB - PRIME DP - Unbound Medicine ER -