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Increased skeletal muscle glucose uptake by rosemary extract through AMPK activation.
Appl Physiol Nutr Metab. 2015 Apr; 40(4):407-13.AP

Abstract

Stimulation of the energy sensor AMP-activated kinase (AMPK) has been viewed as a targeted approach to increase glucose uptake by skeletal muscle and control blood glucose homeostasis. Rosemary extract (RE) has been reported to activate AMPK in hepatocytes and reduce blood glucose levels in vivo but its effects on skeletal muscle are not known. In the present study, we examined the effects of RE and the mechanism of regulation of glucose uptake in muscle cells. RE stimulated glucose uptake in L6 myotubes in a dose- and time-dependent manner. Maximum stimulation was seen with 5 μg/mL of RE for 4 h (184% ± 5.07% of control, p < 0.001), a response comparable to maximum insulin (207% ± 5.26%, p < 0.001) and metformin (216% ± 8.77%, p < 0.001) stimulation. RE did not affect insulin receptor substrate 1 and Akt phosphorylation but significantly increased AMPK and acetyl-CoA carboxylase phosphorylation. Furthermore, the RE-stimulated glucose uptake was significantly reduced by the AMPK inhibitor compound C, but remained unchanged by the PI3K inhibitor, wortmannin. RE did not affect GLUT4 or GLUT1 glucose transporter translocation in contrast with a significant translocation of both transporters seen with insulin or metformin treatment. Our study is the first to show a direct effect of RE on muscle cell glucose uptake by a mechanism that involves AMPK activation.

Authors+Show Affiliations

Department of Health Sciences, Brock University, St. Catharines, ON L2S 3A1, Canada.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25794239

Citation

Naimi, Madina, et al. "Increased Skeletal Muscle Glucose Uptake By Rosemary Extract Through AMPK Activation." Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquee, Nutrition Et Metabolisme, vol. 40, no. 4, 2015, pp. 407-13.
Naimi M, Tsakiridis T, Stamatatos TC, et al. Increased skeletal muscle glucose uptake by rosemary extract through AMPK activation. Appl Physiol Nutr Metab. 2015;40(4):407-13.
Naimi, M., Tsakiridis, T., Stamatatos, T. C., Alexandropoulos, D. I., & Tsiani, E. (2015). Increased skeletal muscle glucose uptake by rosemary extract through AMPK activation. Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquee, Nutrition Et Metabolisme, 40(4), 407-13. https://doi.org/10.1139/apnm-2014-0430
Naimi M, et al. Increased Skeletal Muscle Glucose Uptake By Rosemary Extract Through AMPK Activation. Appl Physiol Nutr Metab. 2015;40(4):407-13. PubMed PMID: 25794239.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Increased skeletal muscle glucose uptake by rosemary extract through AMPK activation. AU - Naimi,Madina, AU - Tsakiridis,Theodoros, AU - Stamatatos,Theocharis C, AU - Alexandropoulos,Dimitris I, AU - Tsiani,Evangelia, Y1 - 2014/12/22/ PY - 2015/3/21/entrez PY - 2015/3/21/pubmed PY - 2016/7/21/medline KW - cellules musculaires KW - energy regulation KW - insulin resistance KW - insulinorésistance KW - metabolism KW - muscle cells KW - métabolisme KW - nutrition KW - régulation énergétique SP - 407 EP - 13 JF - Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme JO - Appl Physiol Nutr Metab VL - 40 IS - 4 N2 - Stimulation of the energy sensor AMP-activated kinase (AMPK) has been viewed as a targeted approach to increase glucose uptake by skeletal muscle and control blood glucose homeostasis. Rosemary extract (RE) has been reported to activate AMPK in hepatocytes and reduce blood glucose levels in vivo but its effects on skeletal muscle are not known. In the present study, we examined the effects of RE and the mechanism of regulation of glucose uptake in muscle cells. RE stimulated glucose uptake in L6 myotubes in a dose- and time-dependent manner. Maximum stimulation was seen with 5 μg/mL of RE for 4 h (184% ± 5.07% of control, p < 0.001), a response comparable to maximum insulin (207% ± 5.26%, p < 0.001) and metformin (216% ± 8.77%, p < 0.001) stimulation. RE did not affect insulin receptor substrate 1 and Akt phosphorylation but significantly increased AMPK and acetyl-CoA carboxylase phosphorylation. Furthermore, the RE-stimulated glucose uptake was significantly reduced by the AMPK inhibitor compound C, but remained unchanged by the PI3K inhibitor, wortmannin. RE did not affect GLUT4 or GLUT1 glucose transporter translocation in contrast with a significant translocation of both transporters seen with insulin or metformin treatment. Our study is the first to show a direct effect of RE on muscle cell glucose uptake by a mechanism that involves AMPK activation. SN - 1715-5320 UR - https://www.unboundmedicine.com/medline/citation/25794239/Increased_skeletal_muscle_glucose_uptake_by_rosemary_extract_through_AMPK_activation_ L2 - http://www.nrcresearchpress.com/doi/full/10.1139/apnm-2014-0430?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -