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The hyperaccumulator Sedum plumbizincicola harbors metal-resistant endophytic bacteria that improve its phytoextraction capacity in multi-metal contaminated soil.
J Environ Manage. 2015 Jun 01; 156:62-9.JE

Abstract

Endophyte-assisted phytoremediation has recently been suggested as a successful approach for ecological restoration of metal contaminated soils, however little information is available on the influence of endophytic bacteria on the phytoextraction capacity of metal hyperaccumulating plants in multi-metal polluted soils. The aims of our study were to isolate and characterize metal-resistant and 1-aminocyclopropane-1-carboxylate (ACC) utilizing endophytic bacteria from tissues of the newly discovered Zn/Cd hyperaccumulator Sedum plumbizincicola and to examine if these endophytic bacterial strains could improve the efficiency of phytoextraction of multi-metal contaminated soils. Among a collection of 42 metal resistant bacterial strains isolated from the tissues of S. plumbizincicola grown on Pb/Zn mine tailings, five plant growth promoting endophytic bacterial strains (PGPE) were selected due to their ability to promote plant growth and to utilize ACC as the sole nitrogen source. The five isolates were identified as Bacillus pumilus E2S2, Bacillus sp. E1S2, Bacillus sp. E4S1, Achromobacter sp. E4L5 and Stenotrophomonas sp. E1L and subsequent testing revealed that they all exhibited traits associated with plant growth promotion, such as production of indole-3-acetic acid and siderophores and solubilization of phosphorus. These five strains showed high resistance to heavy metals (Cd, Zn and Pb) and various antibiotics. Further, inoculation of these ACC utilizing strains significantly increased the concentrations of water extractable Cd and Zn in soil. Moreover, a pot experiment was conducted to elucidate the effects of inoculating metal-resistant ACC utilizing strains on the growth of S. plumbizincicola and its uptake of Cd, Zn and Pb in multi-metal contaminated soils. Out of the five strains, B. pumilus E2S2 significantly increased root (146%) and shoot (17%) length, fresh (37%) and dry biomass (32%) of S. plumbizincicola as well as plant Cd uptake (43%), whereas Bacillus sp. E1S2 significantly enhanced the accumulation of Zn (18%) in plants compared with non-inoculated controls. The inoculated strains also showed high levels of colonization in rhizosphere and plant tissues. Results demonstrate the potential to improve phytoextraction of soils contaminated with multiple heavy metals by inoculating metal hyperaccumulating plants with their own selected functional endophytic bacterial strains.

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Authors+Show Affiliations

Ma Y
Centre for Functional Ecology, Department of Life Sciences, University of Coimbra, Calçada Martim de Freitas, 3000-456 Coimbra, Portugal; Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China. Electronic address: cathymaying@gmail.com.
Oliveira RS
Centre for Functional Ecology, Department of Life Sciences, University of Coimbra, Calçada Martim de Freitas, 3000-456 Coimbra, Portugal; CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Arquiteto Lobão Vital, Apartado 2511, 4202-401 Porto, Portugal; Research Centre on Health and Environment, School of Allied Health Sciences, Polytechnic Institute of Porto, Rua Valente Perfeito, 322, 4400-330 Vila Nova de Gaia, Portugal.
Nai F
Soil Science Agricultural College, Guizhou University, Guiyang 550025, China.
Rajkumar M
Department of Life Sciences, Central University of Tamil Nadu, Collectorate Annexe, Thanjavur Road, Thiruvarur 610 004, Tamil Nadu, India.
Luo Y
Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, Shandong 264003, China.
Rocha I
Centre for Functional Ecology, Department of Life Sciences, University of Coimbra, Calçada Martim de Freitas, 3000-456 Coimbra, Portugal.
Freitas H
Centre for Functional Ecology, Department of Life Sciences, University of Coimbra, Calçada Martim de Freitas, 3000-456 Coimbra, Portugal.

MeSH

BacillusBiodegradation, EnvironmentalBiomassCadmiumEndophytesIndoleacetic AcidsMetals, HeavyPlant RootsRhizosphereSedumSoilSoil PollutantsZinc

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25796039

Citation

Ma, Ying, et al. "The Hyperaccumulator Sedum Plumbizincicola Harbors Metal-resistant Endophytic Bacteria That Improve Its Phytoextraction Capacity in Multi-metal Contaminated Soil." Journal of Environmental Management, vol. 156, 2015, pp. 62-9.
Ma Y, Oliveira RS, Nai F, et al. The hyperaccumulator Sedum plumbizincicola harbors metal-resistant endophytic bacteria that improve its phytoextraction capacity in multi-metal contaminated soil. J Environ Manage. 2015;156:62-9.
Ma, Y., Oliveira, R. S., Nai, F., Rajkumar, M., Luo, Y., Rocha, I., & Freitas, H. (2015). The hyperaccumulator Sedum plumbizincicola harbors metal-resistant endophytic bacteria that improve its phytoextraction capacity in multi-metal contaminated soil. Journal of Environmental Management, 156, 62-9. https://doi.org/10.1016/j.jenvman.2015.03.024
Ma Y, et al. The Hyperaccumulator Sedum Plumbizincicola Harbors Metal-resistant Endophytic Bacteria That Improve Its Phytoextraction Capacity in Multi-metal Contaminated Soil. J Environ Manage. 2015 Jun 1;156:62-9. PubMed PMID: 25796039.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The hyperaccumulator Sedum plumbizincicola harbors metal-resistant endophytic bacteria that improve its phytoextraction capacity in multi-metal contaminated soil. AU - Ma,Ying, AU - Oliveira,Rui S, AU - Nai,Fengjiao, AU - Rajkumar,Mani, AU - Luo,Yongming, AU - Rocha,Inês, AU - Freitas,Helena, Y1 - 2015/03/19/ PY - 2014/11/04/received PY - 2015/03/11/revised PY - 2015/03/13/accepted PY - 2015/3/22/entrez PY - 2015/3/22/pubmed PY - 2016/4/26/medline KW - Endophytic bacteria KW - Heavy metals KW - Hyperaccumulator KW - Phytoextraction KW - Sedum plumbizincicola SP - 62 EP - 9 JF - Journal of environmental management JO - J Environ Manage VL - 156 N2 - Endophyte-assisted phytoremediation has recently been suggested as a successful approach for ecological restoration of metal contaminated soils, however little information is available on the influence of endophytic bacteria on the phytoextraction capacity of metal hyperaccumulating plants in multi-metal polluted soils. The aims of our study were to isolate and characterize metal-resistant and 1-aminocyclopropane-1-carboxylate (ACC) utilizing endophytic bacteria from tissues of the newly discovered Zn/Cd hyperaccumulator Sedum plumbizincicola and to examine if these endophytic bacterial strains could improve the efficiency of phytoextraction of multi-metal contaminated soils. Among a collection of 42 metal resistant bacterial strains isolated from the tissues of S. plumbizincicola grown on Pb/Zn mine tailings, five plant growth promoting endophytic bacterial strains (PGPE) were selected due to their ability to promote plant growth and to utilize ACC as the sole nitrogen source. The five isolates were identified as Bacillus pumilus E2S2, Bacillus sp. E1S2, Bacillus sp. E4S1, Achromobacter sp. E4L5 and Stenotrophomonas sp. E1L and subsequent testing revealed that they all exhibited traits associated with plant growth promotion, such as production of indole-3-acetic acid and siderophores and solubilization of phosphorus. These five strains showed high resistance to heavy metals (Cd, Zn and Pb) and various antibiotics. Further, inoculation of these ACC utilizing strains significantly increased the concentrations of water extractable Cd and Zn in soil. Moreover, a pot experiment was conducted to elucidate the effects of inoculating metal-resistant ACC utilizing strains on the growth of S. plumbizincicola and its uptake of Cd, Zn and Pb in multi-metal contaminated soils. Out of the five strains, B. pumilus E2S2 significantly increased root (146%) and shoot (17%) length, fresh (37%) and dry biomass (32%) of S. plumbizincicola as well as plant Cd uptake (43%), whereas Bacillus sp. E1S2 significantly enhanced the accumulation of Zn (18%) in plants compared with non-inoculated controls. The inoculated strains also showed high levels of colonization in rhizosphere and plant tissues. Results demonstrate the potential to improve phytoextraction of soils contaminated with multiple heavy metals by inoculating metal hyperaccumulating plants with their own selected functional endophytic bacterial strains. SN - 1095-8630 UR - https://www.unboundmedicine.com/medline/citation/25796039/The_hyperaccumulator_Sedum_plumbizincicola_harbors_metal_resistant_endophytic_bacteria_that_improve_its_phytoextraction_capacity_in_multi_metal_contaminated_soil_ DB - PRIME DP - Unbound Medicine ER -