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Calycosin orchestrates the functions of Danggui Buxue Tang, a Chinese herbal decoction composing of Astragali Radix and Angelica Sinensis Radix: An evaluation by using calycosin-knock out herbal extract.
J Ethnopharmacol. 2015 Jun 20; 168:150-7.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Danggui Buxue Tang (DBT) is a classical Chinese herbal decoction containing two herbs, Astragali Radix (AR) and Angelicae Sinensis Radix (ASR), which serves as dietary supplement for treating women menopausal syndromes. Pharmacological studies indicate that DBT has estrogenic, erythropoietic and osteogenic properties; however, the action mechanism for this complex herbal decoction is not known. Calycosin, a major flavonoid in AR, shares similar structure with β-estradiol, and thus which is hypothesized to be the critical compound of DBT. Here, we aim to investigate the role of calycosin in DBT in terms of its biological functions by using a calycosin-depleted DBT decoction (DBT(Δcal)). The biological functions of DBT(Δcal) and parental DBT were systematically compared.

MATERIALS AND METHODS

In order to standardize DBT decoction, four chemical markers were determined and quantified by HPLC. A semi-preparative HPLC method was utilized to prepare DBT(Δcal). The authenticity of DBT(Δcal) was evaluated by LC-QQQ-MS/MS. To reveal the effect of calycosin on DBT functions, several cell assays related to the known properties of DBT were revealed, including estrogenic, erythropoietic and osteogenic functions.

RESULTS

As compared to parental DBT, the estrogenic, erythropoietic and osteogenic abilities were markedly reduced in DBT(Δcal). However, calycosin alone did not show significant responses.

CONCLUSIONS

Our results suggest that calycosin is a bioactive chemical in DBT decoction, and which could play a key linker in orchestrating multi-components of DBT as to achieve maximal functions. These discoveries should be invaluable in drug development and in investigating the modernization of traditional Chinese medicine from a new perspective.

Authors+Show Affiliations

Division of Life Science, Center for Chinese Medicine R&D, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong Special Administrative Region, China.Division of Life Science, Center for Chinese Medicine R&D, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong Special Administrative Region, China.Division of Life Science, Center for Chinese Medicine R&D, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong Special Administrative Region, China.Division of Life Science, Center for Chinese Medicine R&D, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong Special Administrative Region, China.Division of Life Science, Center for Chinese Medicine R&D, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong Special Administrative Region, China.Division of Life Science, Center for Chinese Medicine R&D, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong Special Administrative Region, China.Division of Life Science, Center for Chinese Medicine R&D, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong Special Administrative Region, China.Division of Life Science, Center for Chinese Medicine R&D, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong Special Administrative Region, China.Division of Life Science, Center for Chinese Medicine R&D, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong Special Administrative Region, China.Division of Life Science, Center for Chinese Medicine R&D, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong Special Administrative Region, China.Division of Life Science, Center for Chinese Medicine R&D, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong Special Administrative Region, China. Electronic address: botsim@ust.hk.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

25796405

Citation

Gong, Amy Guo-wei, et al. "Calycosin Orchestrates the Functions of Danggui Buxue Tang, a Chinese Herbal Decoction Composing of Astragali Radix and Angelica Sinensis Radix: an Evaluation By Using Calycosin-knock Out Herbal Extract." Journal of Ethnopharmacology, vol. 168, 2015, pp. 150-7.
Gong AG, Li N, Lau KM, et al. Calycosin orchestrates the functions of Danggui Buxue Tang, a Chinese herbal decoction composing of Astragali Radix and Angelica Sinensis Radix: An evaluation by using calycosin-knock out herbal extract. J Ethnopharmacol. 2015;168:150-7.
Gong, A. G., Li, N., Lau, K. M., Lee, P. S., Yan, L., Xu, M. L., Lam, C. T., Kong, A. Y., Lin, H. Q., Dong, T. T., & Tsim, K. W. (2015). Calycosin orchestrates the functions of Danggui Buxue Tang, a Chinese herbal decoction composing of Astragali Radix and Angelica Sinensis Radix: An evaluation by using calycosin-knock out herbal extract. Journal of Ethnopharmacology, 168, 150-7. https://doi.org/10.1016/j.jep.2015.03.033
Gong AG, et al. Calycosin Orchestrates the Functions of Danggui Buxue Tang, a Chinese Herbal Decoction Composing of Astragali Radix and Angelica Sinensis Radix: an Evaluation By Using Calycosin-knock Out Herbal Extract. J Ethnopharmacol. 2015 Jun 20;168:150-7. PubMed PMID: 25796405.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Calycosin orchestrates the functions of Danggui Buxue Tang, a Chinese herbal decoction composing of Astragali Radix and Angelica Sinensis Radix: An evaluation by using calycosin-knock out herbal extract. AU - Gong,Amy Guo-wei, AU - Li,Ning, AU - Lau,Kei-man, AU - Lee,Pinky Sum-chi, AU - Yan,Lu, AU - Xu,Miranda Li, AU - Lam,Candy Ting-wai, AU - Kong,Ava Yeung-yeung, AU - Lin,Huang-quan, AU - Dong,Tina Ting-xia, AU - Tsim,Karl Wah-keung, Y1 - 2015/03/19/ PY - 2014/10/24/received PY - 2015/01/07/revised PY - 2015/03/10/accepted PY - 2015/3/23/entrez PY - 2015/3/23/pubmed PY - 2016/3/31/medline KW - Angelicae Sinensis Radix KW - Astragali Radix KW - Calycosin KW - Chemical knock-out KW - Danggui Buxue Tang SP - 150 EP - 7 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 168 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Danggui Buxue Tang (DBT) is a classical Chinese herbal decoction containing two herbs, Astragali Radix (AR) and Angelicae Sinensis Radix (ASR), which serves as dietary supplement for treating women menopausal syndromes. Pharmacological studies indicate that DBT has estrogenic, erythropoietic and osteogenic properties; however, the action mechanism for this complex herbal decoction is not known. Calycosin, a major flavonoid in AR, shares similar structure with β-estradiol, and thus which is hypothesized to be the critical compound of DBT. Here, we aim to investigate the role of calycosin in DBT in terms of its biological functions by using a calycosin-depleted DBT decoction (DBT(Δcal)). The biological functions of DBT(Δcal) and parental DBT were systematically compared. MATERIALS AND METHODS: In order to standardize DBT decoction, four chemical markers were determined and quantified by HPLC. A semi-preparative HPLC method was utilized to prepare DBT(Δcal). The authenticity of DBT(Δcal) was evaluated by LC-QQQ-MS/MS. To reveal the effect of calycosin on DBT functions, several cell assays related to the known properties of DBT were revealed, including estrogenic, erythropoietic and osteogenic functions. RESULTS: As compared to parental DBT, the estrogenic, erythropoietic and osteogenic abilities were markedly reduced in DBT(Δcal). However, calycosin alone did not show significant responses. CONCLUSIONS: Our results suggest that calycosin is a bioactive chemical in DBT decoction, and which could play a key linker in orchestrating multi-components of DBT as to achieve maximal functions. These discoveries should be invaluable in drug development and in investigating the modernization of traditional Chinese medicine from a new perspective. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/25796405/Calycosin_orchestrates_the_functions_of_Danggui_Buxue_Tang_a_Chinese_herbal_decoction_composing_of_Astragali_Radix_and_Angelica_Sinensis_Radix:_An_evaluation_by_using_calycosin_knock_out_herbal_extract_ DB - PRIME DP - Unbound Medicine ER -