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Panels of cytokines and other secretory proteins as potential biomarkers of ovarian endometriosis.
J Mol Diagn. 2015 May; 17(3):325-34.JM

Abstract

Endometriosis is a gynecologic disease that is characterized by nonspecific symptoms and invasive diagnostics. To date, there is no adequate noninvasive method for the diagnosis of endometriosis. Although more than 100 potential biomarkers have been investigated in blood and/or peritoneal fluid, none of these has proven useful in clinical practice. The aim to find a suitable panel of biomarkers that would allow noninvasive diagnosis thus remains of interest. We evaluated the concentrations of 16 cytokines and other secretory proteins in serum and peritoneal fluid of 58 women with ovarian endometriosis (cases) and 40 healthy women undergoing sterilization or patients with benign ovarian cysts (controls) using multiplexed double fluorescence-based immunometric assay platform and enzyme-linked immunosorbent assay. Significantly higher concentrations of glycodelin-A were shown in serum, and significantly higher levels of glycodelin-A, IL-6, and IL-8, and lower levels of leptin were measured in the peritoneal fluid of cases versus controls. In serum, the best performance was shown by models that included the ratio of leptin/glycodelin-A and the ratio of ficolin 2/glycodelin-A, whereas in the peritoneal fluid the best models included the ratio of biglycan/leptin, regulated on activation normal T-cell expressed and secreted/IL-6 and ficolin-2/glycodelin-A, and IL-8 per milligram of total protein, all in combination with age. The models using serum and peritoneal fluid distinguished between ovarian endometriosis patients and controls regardless of the menstrual cycle phase with relatively high sensitivity (72.5% to 84.2%), specificity (78.4% to 91.2%), and area under the curve (0.85 to 0.90).

Authors+Show Affiliations

Institute of Biochemistry, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.Institute of Biochemistry, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.Endometriosis Centre, Department of Obstetrics and Gynaecology, Inselspital, University of Berne, Berne, Switzerland.Endometriosis Centre, Department of Obstetrics and Gynaecology, Inselspital, University of Berne, Berne, Switzerland.Institute of Biochemistry, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia. Electronic address: tea.lanisnik-rizner@mf.uni-lj.si.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25797583

Citation

Kocbek, Vida, et al. "Panels of Cytokines and Other Secretory Proteins as Potential Biomarkers of Ovarian Endometriosis." The Journal of Molecular Diagnostics : JMD, vol. 17, no. 3, 2015, pp. 325-34.
Kocbek V, Vouk K, Bersinger NA, et al. Panels of cytokines and other secretory proteins as potential biomarkers of ovarian endometriosis. J Mol Diagn. 2015;17(3):325-34.
Kocbek, V., Vouk, K., Bersinger, N. A., Mueller, M. D., & Lanišnik Rižner, T. (2015). Panels of cytokines and other secretory proteins as potential biomarkers of ovarian endometriosis. The Journal of Molecular Diagnostics : JMD, 17(3), 325-34. https://doi.org/10.1016/j.jmoldx.2015.01.006
Kocbek V, et al. Panels of Cytokines and Other Secretory Proteins as Potential Biomarkers of Ovarian Endometriosis. J Mol Diagn. 2015;17(3):325-34. PubMed PMID: 25797583.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Panels of cytokines and other secretory proteins as potential biomarkers of ovarian endometriosis. AU - Kocbek,Vida, AU - Vouk,Katja, AU - Bersinger,Nick A, AU - Mueller,Michael D, AU - Lanišnik Rižner,Tea, Y1 - 2015/03/20/ PY - 2014/02/07/received PY - 2015/01/01/revised PY - 2015/01/28/accepted PY - 2015/3/24/entrez PY - 2015/3/24/pubmed PY - 2016/1/15/medline SP - 325 EP - 34 JF - The Journal of molecular diagnostics : JMD JO - J Mol Diagn VL - 17 IS - 3 N2 - Endometriosis is a gynecologic disease that is characterized by nonspecific symptoms and invasive diagnostics. To date, there is no adequate noninvasive method for the diagnosis of endometriosis. Although more than 100 potential biomarkers have been investigated in blood and/or peritoneal fluid, none of these has proven useful in clinical practice. The aim to find a suitable panel of biomarkers that would allow noninvasive diagnosis thus remains of interest. We evaluated the concentrations of 16 cytokines and other secretory proteins in serum and peritoneal fluid of 58 women with ovarian endometriosis (cases) and 40 healthy women undergoing sterilization or patients with benign ovarian cysts (controls) using multiplexed double fluorescence-based immunometric assay platform and enzyme-linked immunosorbent assay. Significantly higher concentrations of glycodelin-A were shown in serum, and significantly higher levels of glycodelin-A, IL-6, and IL-8, and lower levels of leptin were measured in the peritoneal fluid of cases versus controls. In serum, the best performance was shown by models that included the ratio of leptin/glycodelin-A and the ratio of ficolin 2/glycodelin-A, whereas in the peritoneal fluid the best models included the ratio of biglycan/leptin, regulated on activation normal T-cell expressed and secreted/IL-6 and ficolin-2/glycodelin-A, and IL-8 per milligram of total protein, all in combination with age. The models using serum and peritoneal fluid distinguished between ovarian endometriosis patients and controls regardless of the menstrual cycle phase with relatively high sensitivity (72.5% to 84.2%), specificity (78.4% to 91.2%), and area under the curve (0.85 to 0.90). SN - 1943-7811 UR - https://www.unboundmedicine.com/medline/citation/25797583/Panels_of_cytokines_and_other_secretory_proteins_as_potential_biomarkers_of_ovarian_endometriosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1525-1578(15)00044-6 DB - PRIME DP - Unbound Medicine ER -