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First-time isolation of Candida dubliniensis from plaque and carious dentine of primary teeth.
Eur Arch Paediatr Dent. 2015 Aug; 16(4):365-70.EA

Abstract

AIM

To determine those organisms of the genus Candida associated with dental caries by investigating samples from active carious lesions. Within the genus Candida, the species Candida albicans and Candida dubliniensis are capable of forming chlamydospores and germ tubes. Until it became possible in 1995 to differentiate between the two species taxonomically, C. dubliniensis was falsely identified as C. albicans. Whilst the importance of C. albicans for rapidly progressing early childhood caries (ECC) has been recognised, so far there have been only reports about C. dubliniensis in connection with children/mothers who have been infected with HIV or already developed AIDS. In the present study, C. dubliniensis was for the first time isolated from plaque and carious dentine of a healthy five-year-old boy.

METHODS

As part of the investigation, a number of samples were collected from individual children affected by active dental caries. Amongst the samples, one in particular indicated that Candida species might be involved. The patient was a five-year-old boy with ECC of the primary dentition, scheduled for restorative treatment under general anaesthesia. Before treatment, a salivary, plaque (region of 54/55) and soft carious dentine sample from the tooth 51 was taken before extraction. The counts of yeasts, lactobacilli (LB) and mutans streptococci were determined in the samples.

RESULTS

The boy's dmft was 11, which was dominated by the d component. In the saliva of the boy, LB and mutans streptococci (MS) were detected. In plaque and carious dentine, MS and most interestingly C. dubliniensis were present. The yeasts were visualised in carious dentine by means of scanning electron micrographs.

CONCLUSIONS

Plaque and carious dentine may be a further habitat of C. dubliniensis.

Authors+Show Affiliations

Biological Laboratory, Clinic for Prosthetic Dentistry and Dental Materials, Centre of Dentistry, University Hospital, Bachstraβe 18, D-07740, Jena, Germany, susanne.kneist@med.uni-jena.de.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Case Reports
Journal Article

Language

eng

PubMed ID

25800500

Citation

Kneist, S, et al. "First-time Isolation of Candida Dubliniensis From Plaque and Carious Dentine of Primary Teeth." European Archives of Paediatric Dentistry : Official Journal of the European Academy of Paediatric Dentistry, vol. 16, no. 4, 2015, pp. 365-70.
Kneist S, Borutta A, Sigusch BW, et al. First-time isolation of Candida dubliniensis from plaque and carious dentine of primary teeth. Eur Arch Paediatr Dent. 2015;16(4):365-70.
Kneist, S., Borutta, A., Sigusch, B. W., Nietzsche, S., Küpper, H., Kostrzewa, M., & Callaway, A. (2015). First-time isolation of Candida dubliniensis from plaque and carious dentine of primary teeth. European Archives of Paediatric Dentistry : Official Journal of the European Academy of Paediatric Dentistry, 16(4), 365-70. https://doi.org/10.1007/s40368-015-0180-1
Kneist S, et al. First-time Isolation of Candida Dubliniensis From Plaque and Carious Dentine of Primary Teeth. Eur Arch Paediatr Dent. 2015;16(4):365-70. PubMed PMID: 25800500.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - First-time isolation of Candida dubliniensis from plaque and carious dentine of primary teeth. AU - Kneist,S, AU - Borutta,A, AU - Sigusch,B W, AU - Nietzsche,S, AU - Küpper,H, AU - Kostrzewa,M, AU - Callaway,A, Y1 - 2015/03/24/ PY - 2014/11/03/received PY - 2015/03/04/accepted PY - 2015/3/25/entrez PY - 2015/3/25/pubmed PY - 2016/1/15/medline SP - 365 EP - 70 JF - European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry JO - Eur Arch Paediatr Dent VL - 16 IS - 4 N2 - AIM: To determine those organisms of the genus Candida associated with dental caries by investigating samples from active carious lesions. Within the genus Candida, the species Candida albicans and Candida dubliniensis are capable of forming chlamydospores and germ tubes. Until it became possible in 1995 to differentiate between the two species taxonomically, C. dubliniensis was falsely identified as C. albicans. Whilst the importance of C. albicans for rapidly progressing early childhood caries (ECC) has been recognised, so far there have been only reports about C. dubliniensis in connection with children/mothers who have been infected with HIV or already developed AIDS. In the present study, C. dubliniensis was for the first time isolated from plaque and carious dentine of a healthy five-year-old boy. METHODS: As part of the investigation, a number of samples were collected from individual children affected by active dental caries. Amongst the samples, one in particular indicated that Candida species might be involved. The patient was a five-year-old boy with ECC of the primary dentition, scheduled for restorative treatment under general anaesthesia. Before treatment, a salivary, plaque (region of 54/55) and soft carious dentine sample from the tooth 51 was taken before extraction. The counts of yeasts, lactobacilli (LB) and mutans streptococci were determined in the samples. RESULTS: The boy's dmft was 11, which was dominated by the d component. In the saliva of the boy, LB and mutans streptococci (MS) were detected. In plaque and carious dentine, MS and most interestingly C. dubliniensis were present. The yeasts were visualised in carious dentine by means of scanning electron micrographs. CONCLUSIONS: Plaque and carious dentine may be a further habitat of C. dubliniensis. SN - 1818-6300 UR - https://www.unboundmedicine.com/medline/citation/25800500/First_time_isolation_of_Candida_dubliniensis_from_plaque_and_carious_dentine_of_primary_teeth_ DB - PRIME DP - Unbound Medicine ER -