Tags

Type your tag names separated by a space and hit enter

Comparison of 2 real-time PCR assays for diagnosis of Pneumocystis jirovecii pneumonia in human immunodeficiency virus (HIV) and non-HIV immunocompromised patients.
Diagn Microbiol Infect Dis. 2015 Jun; 82(2):143-7.DM

Abstract

A total of 120 bronchoalveolar lavage specimens from HIV and non-HIV immunocompromised patients, positive for Pneumocystis jirovecii by an "in house" real-time polymerase chain reaction (PCR), were evaluated by the Bio-Evolution Pneumocystis real-time PCR, a commercial quantitative assay. Patients were classified in 2 categories based on clinical and radiological findings: definite and unlikely Pneumocystis pneumonia (PCP). For the "in house" PCR, cycle threshold 34 was established as cut-off value to discriminate definite PCP from unlikely PCP with 65% and 85% of sensitivity and specificity, respectively. For the Bio-Evolution quantitative PCR, a cut-off value of 2.8×10(5)copies/mL was defined with 72% and 82% of sensitivity and specificity, respectively. Overlapped zones of results for definite and unlikely PCP were observed. Quantitative PCR is probably a useful tool for PCP diagnosis. However, for optimal management of PCP in non-HIV immunocompromised patients, operational thresholds should be assessed according to underlying diseases and other clinical and radiological parameters.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Montesinos I
Microbiology Department, Hopital Erasme, Université Libre de Bruxelles, Route de Lennik 808, 1070 Brussels, Belgium. Electronic address: Carlota.Montesinos@erasme.ulb.ac.be.
Brancart F
Microbiology Department, Hopital Erasme, Université Libre de Bruxelles, Route de Lennik 808, 1070 Brussels, Belgium. Electronic address: Francoise.Brancart@erasme.ulb.ac.be.
Schepers K
Infectious Diseases, Hopital Erasme, Université Libre de Bruxelles, Route de Lennik 808, 1070 Brussels, Belgium. Electronic address: kindaschepers@gmail.com.
Jacobs F
Infectious Diseases, Hopital Erasme, Université Libre de Bruxelles, Route de Lennik 808, 1070 Brussels, Belgium. Electronic address: Frederique.Jacobs@erasme.ulb.ac.be.
Denis O
Microbiology Department, Hopital Erasme, Université Libre de Bruxelles, Route de Lennik 808, 1070 Brussels, Belgium. Electronic address: Olivier.Denis@erasme.ulb.ac.be.
Delforge ML
Microbiology Department, Hopital Erasme, Université Libre de Bruxelles, Route de Lennik 808, 1070 Brussels, Belgium. Electronic address: Marie-Luce.Delforge@erasme.ulb.ac.be.

MeSH

Bronchoalveolar Lavage FluidFemaleHIV InfectionsHumansImmunocompromised HostMaleMiddle AgedMolecular Diagnostic TechniquesPneumocystis cariniiPneumonia, PneumocystisReal-Time Polymerase Chain ReactionSensitivity and Specificity

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25801778

Citation

Montesinos, Isabel, et al. "Comparison of 2 Real-time PCR Assays for Diagnosis of Pneumocystis Jirovecii Pneumonia in Human Immunodeficiency Virus (HIV) and non-HIV Immunocompromised Patients." Diagnostic Microbiology and Infectious Disease, vol. 82, no. 2, 2015, pp. 143-7.
Montesinos I, Brancart F, Schepers K, et al. Comparison of 2 real-time PCR assays for diagnosis of Pneumocystis jirovecii pneumonia in human immunodeficiency virus (HIV) and non-HIV immunocompromised patients. Diagn Microbiol Infect Dis. 2015;82(2):143-7.
Montesinos, I., Brancart, F., Schepers, K., Jacobs, F., Denis, O., & Delforge, M. L. (2015). Comparison of 2 real-time PCR assays for diagnosis of Pneumocystis jirovecii pneumonia in human immunodeficiency virus (HIV) and non-HIV immunocompromised patients. Diagnostic Microbiology and Infectious Disease, 82(2), 143-7. https://doi.org/10.1016/j.diagmicrobio.2015.03.006
Montesinos I, et al. Comparison of 2 Real-time PCR Assays for Diagnosis of Pneumocystis Jirovecii Pneumonia in Human Immunodeficiency Virus (HIV) and non-HIV Immunocompromised Patients. Diagn Microbiol Infect Dis. 2015;82(2):143-7. PubMed PMID: 25801778.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of 2 real-time PCR assays for diagnosis of Pneumocystis jirovecii pneumonia in human immunodeficiency virus (HIV) and non-HIV immunocompromised patients. AU - Montesinos,Isabel, AU - Brancart,Françoise, AU - Schepers,Kinda, AU - Jacobs,Frederique, AU - Denis,Olivier, AU - Delforge,Marie-Luce, Y1 - 2015/03/12/ PY - 2014/08/26/received PY - 2015/03/04/revised PY - 2015/03/05/accepted PY - 2015/3/25/entrez PY - 2015/3/25/pubmed PY - 2016/1/27/medline KW - Pneumocystis jirovecii pneumonia KW - Quantitative real-time PCR SP - 143 EP - 7 JF - Diagnostic microbiology and infectious disease JO - Diagn Microbiol Infect Dis VL - 82 IS - 2 N2 - A total of 120 bronchoalveolar lavage specimens from HIV and non-HIV immunocompromised patients, positive for Pneumocystis jirovecii by an "in house" real-time polymerase chain reaction (PCR), were evaluated by the Bio-Evolution Pneumocystis real-time PCR, a commercial quantitative assay. Patients were classified in 2 categories based on clinical and radiological findings: definite and unlikely Pneumocystis pneumonia (PCP). For the "in house" PCR, cycle threshold 34 was established as cut-off value to discriminate definite PCP from unlikely PCP with 65% and 85% of sensitivity and specificity, respectively. For the Bio-Evolution quantitative PCR, a cut-off value of 2.8×10(5)copies/mL was defined with 72% and 82% of sensitivity and specificity, respectively. Overlapped zones of results for definite and unlikely PCP were observed. Quantitative PCR is probably a useful tool for PCP diagnosis. However, for optimal management of PCP in non-HIV immunocompromised patients, operational thresholds should be assessed according to underlying diseases and other clinical and radiological parameters. SN - 1879-0070 UR - https://www.unboundmedicine.com/medline/citation/25801778/Comparison_of_2_real_time_PCR_assays_for_diagnosis_of_Pneumocystis_jirovecii_pneumonia_in_human_immunodeficiency_virus__HIV__and_non_HIV_immunocompromised_patients_ DB - PRIME DP - Unbound Medicine ER -