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Spatial and temporal regulation of the metabolism of reactive oxygen and nitrogen species during the early development of pepper (Capsicum annuum) seedlings.
Ann Bot. 2015 Sep; 116(4):679-93.AB

Abstract

BACKGROUND AND AIMS

The development of seedlings involves many morphological, physiological and biochemical processes, which are controlled by many factors. Some reactive oxygen and nitrogen species (ROS and RNS, respectively) are implicated as signal molecules in physiological and phytopathological processes. Pepper (Capsicum annuum) is a very important crop and the goal of this work was to provide a framework of the behaviour of the key elements in the metabolism of ROS and RNS in the main organs of pepper during its development.

METHODS

The main seedling organs (roots, hypocotyls and green cotyledons) of pepper seedlings were analysed 7, 10 and 14 d after germination. Activity and gene expression of the main enzymatic antioxidants (catalase, ascorbate-glutathione cycle enzymes), NADP-generating dehydrogenases and S-nitrosoglutathione reductase were determined. Cellular distribution of nitric oxide ((·)NO), superoxide radical (O2 (·-)) and peroxynitrite (ONOO(-)) was investigated using confocal laser scanning microscopy.

KEY RESULTS

The metabolism of ROS and RNS during pepper seedling development was highly regulated and showed significant plasticity, which was co-ordinated among the main seedling organs, resulting in correct development. Catalase showed higher activity in the aerial parts of the seedling (hypocotyls and green cotyledons) whereas roots of 7-d-old seedlings contained higher activity of the enzymatic components of the ascorbate glutathione cycle, NADP-isocitrate dehydrogenase and NADP-malic enzyme.

CONCLUSIONS

There is differential regulation of the metabolism of ROS, nitric oxide and NADP dehydrogenases in the different plant organs during seedling development in pepper in the absence of stress. The metabolism of ROS and RNS seems to contribute significantly to plant development since their components are involved directly or indirectly in many metabolic pathways. Thus, specific molecules such as H2O2 and NO have implications for signalling, and their temporal and spatial regulation contributes to the success of seedling establishment.

Links

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Authors+Show Affiliations

Airaki M
Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, Apartado 419, E-18080 Granada, Spain and.
Leterrier M
Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, Apartado 419, E-18080 Granada, Spain and.
Valderrama R
Group of Biochemistry and Cell Signaling in Nitric Oxide, Department of Biochemistry and Molecular Biology, Campus 'Las Lagunillas', University of Jaén, E-23071 Jaén, Spain.
Chaki M
Group of Biochemistry and Cell Signaling in Nitric Oxide, Department of Biochemistry and Molecular Biology, Campus 'Las Lagunillas', University of Jaén, E-23071 Jaén, Spain.
Begara-Morales JC
Group of Biochemistry and Cell Signaling in Nitric Oxide, Department of Biochemistry and Molecular Biology, Campus 'Las Lagunillas', University of Jaén, E-23071 Jaén, Spain.
Barroso JB
Group of Biochemistry and Cell Signaling in Nitric Oxide, Department of Biochemistry and Molecular Biology, Campus 'Las Lagunillas', University of Jaén, E-23071 Jaén, Spain.
del Río LA
Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, Apartado 419, E-18080 Granada, Spain and.
Palma JM
Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, Apartado 419, E-18080 Granada, Spain and.
Corpas FJ
Group of Antioxidants, Free Radicals and Nitric Oxide in Biotechnology, Food and Agriculture, Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, Apartado 419, E-18080 Granada, Spain and javier.corpas@eez.csic.es.

MeSH

CapsicumNitric OxidePeroxynitrous AcidReactive Nitrogen SpeciesReactive Oxygen SpeciesSeedlingsSuperoxides

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

25808658

Citation

Airaki, Morad, et al. "Spatial and Temporal Regulation of the Metabolism of Reactive Oxygen and Nitrogen Species During the Early Development of Pepper (Capsicum Annuum) Seedlings." Annals of Botany, vol. 116, no. 4, 2015, pp. 679-93.
Airaki M, Leterrier M, Valderrama R, et al. Spatial and temporal regulation of the metabolism of reactive oxygen and nitrogen species during the early development of pepper (Capsicum annuum) seedlings. Ann Bot. 2015;116(4):679-93.
Airaki, M., Leterrier, M., Valderrama, R., Chaki, M., Begara-Morales, J. C., Barroso, J. B., del Río, L. A., Palma, J. M., & Corpas, F. J. (2015). Spatial and temporal regulation of the metabolism of reactive oxygen and nitrogen species during the early development of pepper (Capsicum annuum) seedlings. Annals of Botany, 116(4), 679-93. https://doi.org/10.1093/aob/mcv023
Airaki M, et al. Spatial and Temporal Regulation of the Metabolism of Reactive Oxygen and Nitrogen Species During the Early Development of Pepper (Capsicum Annuum) Seedlings. Ann Bot. 2015;116(4):679-93. PubMed PMID: 25808658.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Spatial and temporal regulation of the metabolism of reactive oxygen and nitrogen species during the early development of pepper (Capsicum annuum) seedlings. AU - Airaki,Morad, AU - Leterrier,Marina, AU - Valderrama,Raquel, AU - Chaki,Mounira, AU - Begara-Morales,Juan C, AU - Barroso,Juan B, AU - del Río,Luis A, AU - Palma,José M, AU - Corpas,Francisco J, Y1 - 2015/03/25/ PY - 2014/10/30/received PY - 2015/02/02/accepted PY - 2015/3/27/entrez PY - 2015/3/27/pubmed PY - 2016/6/18/medline KW - Antioxidant KW - Capsicum annuum KW - NADP dehydrogenases KW - RNS KW - ROS KW - development KW - nitric oxide KW - pepper KW - reactive oxygen species SP - 679 EP - 93 JF - Annals of botany JO - Ann Bot VL - 116 IS - 4 N2 - BACKGROUND AND AIMS: The development of seedlings involves many morphological, physiological and biochemical processes, which are controlled by many factors. Some reactive oxygen and nitrogen species (ROS and RNS, respectively) are implicated as signal molecules in physiological and phytopathological processes. Pepper (Capsicum annuum) is a very important crop and the goal of this work was to provide a framework of the behaviour of the key elements in the metabolism of ROS and RNS in the main organs of pepper during its development. METHODS: The main seedling organs (roots, hypocotyls and green cotyledons) of pepper seedlings were analysed 7, 10 and 14 d after germination. Activity and gene expression of the main enzymatic antioxidants (catalase, ascorbate-glutathione cycle enzymes), NADP-generating dehydrogenases and S-nitrosoglutathione reductase were determined. Cellular distribution of nitric oxide ((·)NO), superoxide radical (O2 (·-)) and peroxynitrite (ONOO(-)) was investigated using confocal laser scanning microscopy. KEY RESULTS: The metabolism of ROS and RNS during pepper seedling development was highly regulated and showed significant plasticity, which was co-ordinated among the main seedling organs, resulting in correct development. Catalase showed higher activity in the aerial parts of the seedling (hypocotyls and green cotyledons) whereas roots of 7-d-old seedlings contained higher activity of the enzymatic components of the ascorbate glutathione cycle, NADP-isocitrate dehydrogenase and NADP-malic enzyme. CONCLUSIONS: There is differential regulation of the metabolism of ROS, nitric oxide and NADP dehydrogenases in the different plant organs during seedling development in pepper in the absence of stress. The metabolism of ROS and RNS seems to contribute significantly to plant development since their components are involved directly or indirectly in many metabolic pathways. Thus, specific molecules such as H2O2 and NO have implications for signalling, and their temporal and spatial regulation contributes to the success of seedling establishment. SN - 1095-8290 UR - https://www.unboundmedicine.com/medline/citation/25808658/Spatial_and_temporal_regulation_of_the_metabolism_of_reactive_oxygen_and_nitrogen_species_during_the_early_development_of_pepper__Capsicum_annuum__seedlings_ DB - PRIME DP - Unbound Medicine ER -