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Effects of TBEP on the induction of oxidative stress and endocrine disruption in Tm3 Leydig cells.
Environ Toxicol. 2016 Oct; 31(10):1276-86.ET

Abstract

The flame retardant tris (2-butoxyethyl) phosphate (TBEP) is a frequently detected contaminant in the environment. In the cultured TM3 cells (originated from ATCC), effects of TBEP on the induction of oxidative stress and endocrine disruption were evaluated. It was observed that exposure to 100 μg/mL TBEP for 24 h significantly reduced the viability of TM3 cells. The mRNA levels of genes related to oxidative stress including Sod, Gpx1, Cat, and Gsta1 were changed in a dose-dependent and/or time-dependent manner after exposure to 30 and 100 μg/mL TBEP for 6, 12, or 24 h. Moreover, notable decrease in glutathione (GSH) contents and increases in oxidized glutathione (GSSG) contents as well as the antioxidant enzyme activities like superoxide dismutase, catalase, glutathione peroxidase, and glutathione S-transferase were found in the group treated with 100 μg/mL TBEP for 24 h, indicating that TBEP induced oxidative stress in TM3 Leydig cells. In addition, the expression of genes related to testosterone (T) synthesis including cytochrome P450 cholesterol side-chain cleavage enzyme (P450scc), cytochrome P450 17α-hydroxysteroid dehydrogenase (P450-17α), and 17β-hydroxysteroid dehydrogenase (17β-HSD) and T levels in medium were remarkably declined by the treatment of 100 μg/mL TBEP for 24 h. And TBEP could inhibit the expression of P450-17α and 17β-HSD and T levels up-regulated by hCG in TM3 cells. Taken together, these findings indicated that TBEP can induce oxidative stress and alter steroidogenesis in TM3 cells. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1276-1286, 2016.

Authors+Show Affiliations

Department of Biotechnology, College of Biological and Environmental Engineering, Zhejiang University of Technology, Zhejiang, Hangzhou, 310032, China.Department of Biotechnology, College of Biological and Environmental Engineering, Zhejiang University of Technology, Zhejiang, Hangzhou, 310032, China.Department of Biotechnology, College of Biological and Environmental Engineering, Zhejiang University of Technology, Zhejiang, Hangzhou, 310032, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

25808963

Citation

Jin, Yuanxiang, et al. "Effects of TBEP On the Induction of Oxidative Stress and Endocrine Disruption in Tm3 Leydig Cells." Environmental Toxicology, vol. 31, no. 10, 2016, pp. 1276-86.
Jin Y, Chen G, Fu Z. Effects of TBEP on the induction of oxidative stress and endocrine disruption in Tm3 Leydig cells. Environ Toxicol. 2016;31(10):1276-86.
Jin, Y., Chen, G., & Fu, Z. (2016). Effects of TBEP on the induction of oxidative stress and endocrine disruption in Tm3 Leydig cells. Environmental Toxicology, 31(10), 1276-86. https://doi.org/10.1002/tox.22137
Jin Y, Chen G, Fu Z. Effects of TBEP On the Induction of Oxidative Stress and Endocrine Disruption in Tm3 Leydig Cells. Environ Toxicol. 2016;31(10):1276-86. PubMed PMID: 25808963.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of TBEP on the induction of oxidative stress and endocrine disruption in Tm3 Leydig cells. AU - Jin,Yuanxiang, AU - Chen,Guanliang, AU - Fu,Zhengwei, Y1 - 2015/03/24/ PY - 2014/12/23/received PY - 2015/02/27/revised PY - 2015/03/07/accepted PY - 2015/3/27/entrez PY - 2015/3/27/pubmed PY - 2017/2/23/medline KW - TM3 cell KW - endocrine disruption KW - flame retardant KW - oxidative stress KW - tris (2-butoxyethyl) phosphate SP - 1276 EP - 86 JF - Environmental toxicology JO - Environ Toxicol VL - 31 IS - 10 N2 - The flame retardant tris (2-butoxyethyl) phosphate (TBEP) is a frequently detected contaminant in the environment. In the cultured TM3 cells (originated from ATCC), effects of TBEP on the induction of oxidative stress and endocrine disruption were evaluated. It was observed that exposure to 100 μg/mL TBEP for 24 h significantly reduced the viability of TM3 cells. The mRNA levels of genes related to oxidative stress including Sod, Gpx1, Cat, and Gsta1 were changed in a dose-dependent and/or time-dependent manner after exposure to 30 and 100 μg/mL TBEP for 6, 12, or 24 h. Moreover, notable decrease in glutathione (GSH) contents and increases in oxidized glutathione (GSSG) contents as well as the antioxidant enzyme activities like superoxide dismutase, catalase, glutathione peroxidase, and glutathione S-transferase were found in the group treated with 100 μg/mL TBEP for 24 h, indicating that TBEP induced oxidative stress in TM3 Leydig cells. In addition, the expression of genes related to testosterone (T) synthesis including cytochrome P450 cholesterol side-chain cleavage enzyme (P450scc), cytochrome P450 17α-hydroxysteroid dehydrogenase (P450-17α), and 17β-hydroxysteroid dehydrogenase (17β-HSD) and T levels in medium were remarkably declined by the treatment of 100 μg/mL TBEP for 24 h. And TBEP could inhibit the expression of P450-17α and 17β-HSD and T levels up-regulated by hCG in TM3 cells. Taken together, these findings indicated that TBEP can induce oxidative stress and alter steroidogenesis in TM3 cells. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1276-1286, 2016. SN - 1522-7278 UR - https://www.unboundmedicine.com/medline/citation/25808963/Effects_of_TBEP_on_the_induction_of_oxidative_stress_and_endocrine_disruption_in_Tm3_Leydig_cells_ L2 - https://doi.org/10.1002/tox.22137 DB - PRIME DP - Unbound Medicine ER -